Institution
National University of Cuyo
Education•Mendoza, Argentina•
About: National University of Cuyo is a education organization based out in Mendoza, Argentina. It is known for research contribution in the topics: Population & Exocytosis. The organization has 3175 authors who have published 4872 publications receiving 83221 citations. The organization is also known as: National University of Cuyo.
Topics: Population, Exocytosis, Autophagy, Magnetization, Endosome
Papers published on a yearly basis
Papers
More filters
••
TL;DR: This review focuses on studies with bacteria for which biosynthesis/production of the plant hormones gibberellins have been demonstrated and the potential involvement of symbiotic and soil-endophytic microorganisms in plant growth promotion and yield increase.
Abstract: This review focuses on studies with bacteria for which biosynthesis/production of the plant hormones gibberellins have been demonstrated. Actual data on gibberellin metabolism by bacteria are analyzed in comparison with the biosynthetic pathways known for vascular plants and fungi. The potential involvement of gibberellins produced by symbiotic and soil-endophytic microorganisms in plant growth promotion and yield increase is also discussed.
466 citations
••
TL;DR: The results demonstrate that in K562 cells Rab11 modulates the exosome pathway although the exact step involved is still not known.
Abstract: During maturation, reticulocytes lose some membrane proteins that are not required on the mature red cell surface. The proteins are released into the extracellular medium associated with vesicles that are formed by budding of the endosomal membrane into the lumen of the compartment; this process results in the formation of multivesicular bodies (MVBs). Fusion of MVBs with the plasma membrane results in secretion of the small internal vesicles, termed exosomes. K562 cells release exosomes with similar characteristics to reticulocyte exosomes, in particular the transferrin receptor (TfR) is found associated with the vesicles. Interestingly, this cell line has been shown to possess high amounts of Rab11 compared with other Rab proteins. To assess the regulation of transferrin receptor release via exosome secretion by Rab11 in this cell type, K562 cells were stably transfected with GFP-Rab11wt or the GTP- and GDP-locked mutants. The distribution of the proteins was assessed by fluorescence microscopy. Transferrin recycling and the number of TfRs present on the surface of the transfected cells were reduced by overexpression of either Rab11wt or the mutants. The amount of released exosomes was analyzed by measuring different molecular markers present on these vesicles either biochemically or by western blot. Overexpression of the dominant-negative mutant Rab11S25N inhibited exosome release, whereas the secretion of exosomes was slightly stimulated in cells transfected with Rab11wt. Taken together, the results demonstrate that in K562 cells Rab11 modulates the exosome pathway although the exact step involved is still not known.
451 citations
••
TL;DR: A rise in intracellular calcium concentration enhanced exosome secretion in Rab11 wt overexpressing cells and reversed the inhibition of the mutants, suggesting that both Rab11 and calcium are involved in the homotypic fusion of MVBs.
Abstract: Multivesicular bodies (MVBs) are membranous structures within 60-100 nm diameter vesicles accumulate. MVBs are generated after invagination and pinching off of the endosomal membrane in the lumen of the vacuole. In certain cell types, fusion of MVBs with the plasma membrane results in the release of the internal vesicles called exosomes. In this report we have examined how an increase in cytosolic calcium affects the development of MVBs and exosome release in K562 cells overexpressing GFP-Rab11 wt or its mutants. In cells overexpressing the Rab11Q70 L mutant or Rab11 wt, an increase in the cytosolic calcium concentration induced by monensin caused a marked enlargement of the MVBs. This effect was abrogated by the membrane permeant calcium chelator BAPTA-AM. We also examined the behavior of MVBs in living cells by time lapse confocal microscopy. Many MVBs, decorated by wt or Q70L mutant GFP-Rab11, were docked and ready to fuse in the presence of a calcium chelator. This observation suggests that Rab11 is acting in the tethering/docking of MVBs to promote homotypic fusion, but that the final fusion reaction requires the presence of calcium. Additionally, a rise in intracellular calcium concentration enhanced exosome secretion in Rab11 wt overexpressing cells and reversed the inhibition of the mutants. The results suggest that both Rab11 and calcium are involved in the homotypic fusion of MVBs.
445 citations
••
TL;DR: Evidence is presented indicating that VAMP3 is required for the fusion between MVBs with autophagosomes to generate the amphisome, allowing the maturation of the autophagic pathway, but it does not seem to be involved in the next step, i.
443 citations
••
TL;DR: This review highlights the most recent advances in the understanding of the molecular machinery that participates in MVB biogenesis and regulates the interplay between autophagy and this organelle.
Abstract: In the majority of cell types, multivesicular bodies (MVBs) are a special kind of late endosomes, crucial intermediates in the internalization of nutrients, ligands and receptors through the endolysosomal system. ESCRT-0, I, II and III (endosomal sorting complex required for transport) are involved in the sorting of proteins into MVBs, generating the intraluminal vesicles. Autophagy is a lysosomal degradation pathway for cytoplasmic components such as proteins and organelles. The autophagosome, a well-characterized structure of the autophagy pathway, can fuse with endocytic structures such as MVBs to generate the amphisome. Finally, the amphisome fuses with the lysosome to degrade the material wrapped inside. Currently, clear evidence suggests that efficient autophagic degradation requires functional MVBs. This review highlights the most recent advances in our understanding of the molecular machinery that participates in MVB biogenesis and regulates the interplay between autophagy and this organelle.
423 citations
Authors
Showing all 3213 results
Name | H-index | Papers | Citations |
---|---|---|---|
David G. Bostwick | 99 | 403 | 31638 |
Elbio Dagotto | 67 | 533 | 27172 |
Facundo Manes | 66 | 245 | 18946 |
Marcela Carena | 63 | 192 | 40884 |
Daniel Batlle | 58 | 243 | 11557 |
M. Gómez Berisso | 58 | 221 | 13924 |
Agustín Ibáñez | 54 | 337 | 9032 |
Leonid V. Zhigilei | 52 | 194 | 9965 |
David M. Spooner | 51 | 187 | 8974 |
Hernán Asorey | 51 | 171 | 11047 |
Raúl A. Baragiola | 48 | 231 | 7932 |
Gerardo F. Goya | 48 | 201 | 8972 |
María Isabel Colombo | 48 | 231 | 18322 |
Vittorio Erspamer | 48 | 152 | 9666 |
Ramon Codina | 47 | 210 | 8199 |