National University of Malaysia

About: National University of Malaysia is a education organization based out in Kuala Lumpur, Malaysia. It is known for research contribution in the topics: Population & Heat transfer. The organization has 26593 authors who have published 41270 publications receiving 552683 citations. The organization is also known as: NUM & Universiti Kebangsaan Malaysia.

Cross-Comparison of Exome Analysis, Next-Generation Sequencing of Amplicons, and the iPLEX® ADME PGx Panel for Pharmacogenomic Profiling

Abstract: Whole-exome sequencing (WES) has been widely used for analysis of human genetic diseases, but its value for the pharmacogenomic profiling of individuals is not well studied. Initially, we performed an in-depth evaluation of the accuracy of WES variant calling in the pharmacogenes CYP2D6 and CYP2C19 by comparison with MiSeq(®) amplicon sequencing data (n = 36). This analysis revealed that the concordance rate between WES and MiSeq(®) was high, achieving 99.60% for variants that were called without exceeding the truth-sensitivity threshold (99%), defined during variant quality score recalibration (VQSR). Beyond this threshold, the proportion of discordant calls increased markedly. Subsequently, we expanded our findings beyond CYP2D6 and CYP2C19 to include more genes genotyped by the iPLEX(®) ADME PGx Panel in the subset of twelve samples. WES performed well, agreeing with the genotyping panel in approximately 99% of the selected pass-filter variant calls. Overall, our results have demonstrated WES to be a promising approach for pharmacogenomic profiling, with an estimated error rate of lower than 1%. Quality filters, particularly VQSR, are important for reducing the number of false variants. Future studies may benefit from examining the role of WES in the clinical setting for guiding drug therapy.

The role of window glazing on daylighting and energy saving in buildings

Abstract: Energy conservation in building arena is essential issue for achieving sustainable environment. However, buildings experienced significant amount of heat gain or loss through window and this will affect the thermal comfort of buildings׳ occupants. Building without window is able to save energy, but it is not recommended due to the benefits of natural light on visual comfort and the biological effect of natural light on humans. Hence, window design plays important role in building architect. One of the essential parts of window is the glazing. Selecting a window glazing is complicated when energy saving and daylighting aspects of a building are considered concurrently. Optimization techniques offer a balance solution for the contradictions in selecting a window glazing of energy-efficient building. This paper intended to reveal the impacts of window glazing on the energy and daylighting performances of building through the previous researches. Then, the optimization techniques used by various researchers in choosing a glazing are highlighted. The emerging glazing technologies were discussed as well.