North Carolina State University

About: North Carolina State University is a education organization based out in Raleigh, North Carolina, United States. It is known for research contribution in the topics: Population & Thin film. The organization has 44161 authors who have published 101744 publications receiving 3456774 citations. The organization is also known as: NCSU & North Carolina State University at Raleigh.

High-throughput gene and SNP discovery in Eucalyptus grandis, an uncharacterized genome

Abstract: Benefits from high-throughput sequencing using 454 pyrosequencing technology may be most apparent for species with high societal or economic value but few genomic resources. Rapid means of gene sequence and SNP discovery using this novel sequencing technology provide a set of baseline tools for genome-level research. However, it is questionable how effective the sequencing of large numbers of short reads for species with essentially no prior gene sequence information will support contig assemblies and sequence annotation. With the purpose of generating the first broad survey of gene sequences in Eucalyptus grandis, the most widely planted hardwood tree species, we used 454 technology to sequence and assemble 148 Mbp of expressed sequences (EST). EST sequences were generated from a normalized cDNA pool comprised of multiple tissues and genotypes, promoting discovery of homologues to almost half of Arabidopsis genes, and a comprehensive survey of allelic variation in the transcriptome. By aligning the sequencing reads from multiple genotypes we detected 23,742 SNPs, 83% of which were validated in a sample. Genome-wide nucleotide diversity was estimated for 2,392 contigs using a modified theta (θ) parameter, adapted for measuring genetic diversity from polymorphisms detected by randomly sequencing a multi-genotype cDNA pool. Diversity estimates in non-synonymous nucleotides were on average 4x smaller than in synonymous, suggesting purifying selection. Non-synonymous to synonymous substitutions (Ka/Ks) among 2,001 contigs averaged 0.30 and was skewed to the right, further supporting that most genes are under purifying selection. Comparison of these estimates among contigs identified major functional classes of genes under purifying and diversifying selection in agreement with previous researches. In providing an abundance of foundational transcript sequences where limited prior genomic information existed, this work created part of the foundation for the annotation of the E. grandis genome that is being sequenced by the US Department of Energy. In addition we demonstrated that SNPs sampled in large-scale with 454 pyrosequencing can be used to detect evolutionary signatures among genes, providing one of the first genome-wide assessments of nucleotide diversity and Ka/Ks for a non-model plant species.

Agent communication languages: rethinking the principles

Abstract: Agent communication languages have been used for years in proprietary multiagent systems. Yet agents from different vendors-or even different research projects-cannot communicate with each other. The author looks at the underlying reasons and proposes a conceptual shift from individual agent representations to social interaction.

Wavelet-Based Approach for Structural Damage Detection

Abstract: A wavelet-based approach is proposed for structural damage detection and health monitoring. Characteristics of representative vibration signals under the wavelet transformation are examined. The methodology is then applied to simulation data generated from a simple structural model subjected to a harmonic excitation. The model consists of multiple breakable springs, some of which may suffer irreversible damage when the response exceeds a threshold value or the number of cycles of motion is accumulated beyond their fatigue life. In cases of either abrupt or accumulative damages, occurrence of damage and the moment when it occurs can be clearly determined in the details of the wavelet decomposition of these data. Similar results are observed for the real acceleration data of the seismic response recorded on the roof of a building during the 1971 San Fernando earthquake. Effects of noise intensity and damage severity are investigated and presented by a detectability map. Results show the great promise of the wavelet approach for damage detection and structural health monitoring.