scispace - formally typeset
Search or ask a question
Institution

Ochsner Medical Center

HealthcareNew Orleans, Louisiana, United States
About: Ochsner Medical Center is a healthcare organization based out in New Orleans, Louisiana, United States. It is known for research contribution in the topics: Population & Heart failure. The organization has 980 authors who have published 1159 publications receiving 49961 citations. The organization is also known as: Ochsner Hospital & Ochsner Foundation Hospital.


Papers
More filters
Journal ArticleDOI
TL;DR: On the basis of the preliminary evidence of efficacy and the consistent safety profile in this patient group, it is proposed that experienced multidisciplinary NET teams may consider PRRT alongside everolimus as an option for patients with advanced somatostatin receptor-positive lung typical/atypical carcinoids whose disease is progressing during first-line treatment with som atostatin analogues.

27 citations

Journal ArticleDOI
TL;DR: Novel methods to purify and detect MVs shed from endothelial cells (ECs) and endothelial progenitor cells (EPCs) by combining microbeads with fluorescence quantum dots (Q-dots) coupled nanoparticle tracking analysis (NTA).
Abstract: Accurate analysis of specific microvesicles (MVs) from biofluids is critical and challenging. Here we described novel methods to purify and detect MVs shed from endothelial cells (ECs) and endothelial progenitor cells (EPCs) by combining microbeads with fluorescence quantum dots (Q-dots) coupled nanoparticle tracking analysis (NTA). In the in vitro screening systems, we demonstrated that 1) anti-CD105 (EC marker) and anti-CD34 (EPC marker) conjugated-microbeads had the highest sensitivity and specificity for isolating respective MVs, which were confirmed with negative controls, CD41 and CD235a; 2) anti-CD144 (EC marker) and anti-KDR (EPC marker) conjugated-Q-dots exhibited the best sensitivity and specificity for their respective MV NTA detection, which were confirmed with positive control, anti-Annexin V (MV universal marker). The methods were further validated by their ability to efficiently recover the known amount of EC-MVs and EPC-MVs from particle-depleted plasma, and to detect the dynamical changes of plasma MVs in ischemic stroke patients, as compared with traditional flow cytometry. These novel methods provide ideal approaches for functional analysis and biomarker discovery of ECs- and EPCs- derived MVs.

27 citations

Journal ArticleDOI
TL;DR: The results not only show that HO-1-generated hydrogen peroxide leads to a decrease inHO-1 activity but also provide for a chemically defined system to be used to examine the function of full-length HO- 1 in a membrane environment.
Abstract: Heme oxygenase (HO) catalyzes heme degradation in a reaction requiring NADPH-cytochrome P450 reductase (CPR). Although most studies with HO used a soluble 30-kDa form, lacking the C-terminal membrane-binding region, recent reports show that the catalytic behavior of this enzyme is very different if this domain is retained; the overall activity was elevated 5-fold, and the Km for CPR decreased approximately 50-fold. The goal of these studies was to accurately measure HO activity using a coupled assay containing purified biliverdin reductase (BVR). This allows measurement of bilirubin formation after incorporation of full-length CPR and heme oxygenase-1 (HO-1) into a membrane environment. When rat liver cytosol was used as the source of partially purified BVR, the reaction remained linear for 2 to 3 min; however, the reaction was only linear for 10 to 30 s when an equivalent amount of purified, human BVR (hBVR) was used. This lack of linearity was not observed with soluble HO-1. Optimal formation of bilirubin was achieved with concentrations of bovine serum albumin (0.25 mg/ml) and hBVR (0.025–0.05 μM), but neither supplement increased the time that the reaction remained linear. Various concentrations of superoxide dismutase had no effect on the reaction; however, when catalase was included, the reactions were linear for at least 4 to 5 min, even at high CPR levels. These results not only show that HO-1-generated hydrogen peroxide leads to a decrease in HO-1 activity but also provide for a chemically defined system to be used to examine the function of full-length HO-1 in a membrane environment.

27 citations

Journal ArticleDOI
TL;DR: In this article, the authors compared the clinical profiles of patients referred for carotid artery stenting (CAS) versus CEA in a large national database, and investigated the comparability of CAS and CEA patients, stratified the cohort into quintiles of the propensity score for r...
Abstract: Background—Carotid artery stenting (CAS) and carotid endarterectomy (CEA) are alternative strategies for stroke prevention in patients with atherosclerotic carotid disease. Although randomized clinical trials are the gold standard for assessing the relative benefits of different treatments, observational research is necessary for determining “real-world” effectiveness. Current recommendations limit the application of CAS to high-risk patients, undermining the ability to “balance” the characteristics of patients treated with either approach. We compared the clinical profiles of patients referred for CAS versus CEA in a large national database. Methods and Results—Clinical characteristics of 12 701 patients referred for CAS or CEA in the National Cardiovascular Data Registry–Carotid Artery Revascularization and Endarterectomy were compared for 44 clinical and demographic variables. To investigate the comparability of CAS and CEA patients, we stratified the cohort into quintiles of the propensity score for r...

27 citations


Authors

Showing all 993 results

NameH-indexPapersCitations
Carl J. Lavie106113549318
Michael R. Jaff8244228891
Michael F. O'Rourke8145135355
Mandeep R. Mehra8064431939
Richard V. Milani8045423410
Christopher J. White7762125767
Bruce A. Reitz7433318457
Robert C. Bourge6927324397
Sana M. Al-Khatib6937717370
Hector O. Ventura6647816379
Andrew Mason6336015198
Aaron S. Dumont6038613020
Philip J. Kadowitz5537911951
David W. Dunn541958999
Lydia A. Bazzano5126713581
Network Information
Related Institutions (5)
Henry Ford Hospital
12.4K papers, 465.3K citations

80% related

Cleveland Clinic
79.3K papers, 3.4M citations

77% related

Cedars-Sinai Medical Center
26.4K papers, 1.2M citations

77% related

Vanderbilt University Medical Center
34.6K papers, 1.1M citations

76% related

Hospital of the University of Pennsylvania
14K papers, 459.9K citations

76% related

Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20231
202223
2021120
2020117
2019102
201886