Institution
Osaka University
Education•Osaka, Japan•
About: Osaka University is a education organization based out in Osaka, Japan. It is known for research contribution in the topics: Laser & Catalysis. The organization has 83778 authors who have published 185669 publications receiving 5158122 citations. The organization is also known as: Ōsaka daigaku.
Topics: Laser, Catalysis, Population, Gene, Thin film
Papers published on a yearly basis
Papers
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National Institutes of Natural Sciences, Japan1, National Institute of Information and Communications Technology2, Raman Research Institute3, Waseda University4, Osaka Institute of Technology5, Kyoto University6, Osaka City University7, Japan Aerospace Exploration Agency8, University of Electro-Communications9, Kindai University10, National Institute of Advanced Industrial Science and Technology11, Tokyo Institute of Technology12, Goddard Space Flight Center13, University of Tokyo14, Hiroshima University15, Ochanomizu University16, Liverpool John Moores University17, Nagoya University18, Nihon University19, Rikkyo University20, Tokyo Keizai University21, Yamanashi Eiwa College22, Rochester Institute of Technology23, Stanford University24, California Institute of Technology25, Hirosaki University26, Niigata University27, Tokai University28, Tohoku University29, Osaka University30, National Defense Academy of Japan31, University of Tübingen32, Hosei University33, University of Wisconsin–Milwaukee34, Tokyo University of Science35, University of Birmingham36
614 citations
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TL;DR: The MALP-2-induced activation of intracellular signaling molecules was fully dependent on both TLR2 and MyD88, and there was a strong preference for the R-MALP in the recognition by its functional receptor,TLR2.
Abstract: Mycoplasmas and their membranes are potent activators of macrophages, the active principle being lipoproteins and lipopeptides. Two stereoisomers of the mycoplasmal lipopeptide macrophage-activating lipopeptide-2 (MALP-2) differing in the configuration of the lipid moiety were synthesized and compared in their macrophage-activating potential, the R-MALP being >100 times more active than the S-MALP in stimulating the release of cytokines, chemokines, and NO. To assess the role of the Toll-like receptor (TLR) family in mycoplasmal lipopeptide signaling, the MALP-2-mediated responses were analyzed using macrophages from wild-type, TLR2-, TLR4-, and MyD88-deficient mice. TLR2- and MyD88-deficient cells showed severely impaired cytokine productions in response to R- and S-MALP. The MALP-induced activation of intracellular signaling molecules was fully dependent on both TLR2 and MyD88. There was a strong preference for the R-MALP in the recognition by its functional receptor, TLR2.
614 citations
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TL;DR: P piggyBac transposon–based reprogramming may be used to generate therapeutically applicable iPSCs and could be identified by negative selection.
Abstract: Induced pluripotent stem cells (iPSCs) have been generated from somatic cells by transgenic expression of Oct4 (Pou5f1), Sox2, Klf4 and Myc. A major difficulty in the application of this technology for regenerative medicine, however, is the delivery of reprogramming factors. Whereas retroviral transduction increases the risk of tumorigenicity, transient expression methods have considerably lower reprogramming efficiencies. Here we describe an efficient piggyBac transposon-based approach to generate integration-free iPSCs. Transposons carrying 2A peptide-linked reprogramming factors induced reprogramming of mouse embryonic fibroblasts with equivalent efficiencies to retroviral transduction. We removed transposons from these primary iPSCs by re-expressing transposase. Transgene-free iPSCs could be identified by negative selection. piggyBac excised without a footprint, leaving the iPSC genome without any genetic alteration. iPSCs fulfilled all criteria of pluripotency, such as pluripotency gene expression, teratoma formation and contribution to chimeras. piggyBac transposon-based reprogramming may be used to generate therapeutically applicable iPSCs.
613 citations
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TL;DR: Hypoadiponectinemia is a marker for predisposition to hypertension in men and blood pressure was inversely associated with adiponectin concentration in normotensives regardless of insulin resistance.
Abstract: Adiponectin is one of the key molecules in the metabolic syndrome, and its concentration is decreased in obesity, type-2 diabetes, and coronary artery disease. Genetic investigation has revealed th...
613 citations
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TL;DR: In this paper, the presence of structure-dependent edge states of graphite is revealed by both ambient and ultra-high-vacuum (UHV) scanning tunneling microscopy and scan tunneling spectroscopy observations.
Abstract: The presence of structure-dependent edge states of graphite is revealed by both ambient and ultrahigh-vacuum (UHV) scanning tunneling microscopy and scanning tunneling spectroscopy observations. On a hydrogenated zigzag (armchair) edge, bright spots are (are not) observed together with a $(\sqrt{3}\ifmmode\times\else\texttimes\fi{}\sqrt{3})R30\ifmmode^\circ\else\textdegree\fi{}$ superlattice near the Fermi level (${V}_{S}\ensuremath{\sim}\ensuremath{-}30\phantom{\rule{0.3em}{0ex}}\mathrm{mV}$ for a peak of the local density of states) under UHV, demonstrating that a zigzag edge is responsible for the edge states, although there is no appreciable difference between as-prepared zigzag and armchair edges in air. Even in the hydrogenated armchair edge, however, bright spots are observed at defect points, at which partial zigzag edges are created in the armchair edge.
613 citations
Authors
Showing all 84130 results
Name | H-index | Papers | Citations |
---|---|---|---|
Shizuo Akira | 261 | 1308 | 320561 |
Thomas C. Südhof | 191 | 653 | 118007 |
Tadamitsu Kishimoto | 181 | 1067 | 130860 |
Yusuke Nakamura | 179 | 2076 | 160313 |
H. S. Chen | 179 | 2401 | 178529 |
Hyun-Chul Kim | 176 | 4076 | 183227 |
Masayuki Yamamoto | 171 | 1576 | 123028 |
Kenji Kangawa | 153 | 1117 | 110059 |
Jongmin Lee | 150 | 2257 | 134772 |
Yoshio Bando | 147 | 1234 | 80883 |
Takeo Kanade | 147 | 799 | 103237 |
Olaf Reimer | 144 | 716 | 74359 |
Yuji Matsuzawa | 143 | 836 | 116711 |
Kim Nasmyth | 142 | 294 | 59231 |
Tasuku Honjo | 141 | 712 | 88428 |