Showing papers by "Polytechnic University of Valencia published in 2003"

Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers.

Abstract: Cucurbita pepo is a highly polymorphic species. The cultivars can be grouped into eight morphotypes in two subspecies, ssp. pepo and ssp. ovifera. A collection of 69 accessions representative of the morphotypes and some unclassified types was used for analysing the morphological and molecular diversity of this species. This collection includes commercial cultivars and Spanish landraces, which represent the great diversification of types that have arisen in Europe after this species arrived from America. For the molecular variability studies, two PCR-based systems were employed, AFLP and SRAP, which preferentially amplify ORFs. Principal coordinates analysis and cluster analysis using the UPGMA method clearly separate the accessions into the two subspecies through the use of both markers. However, the gene diversity and the genetic identity values among morphotypes and subspecies varied between the two marker systems. The information given by SRAP markers was more concordant to the morphological variability and to the evolutionary history of the morphotypes than that of AFLP markers. In ssp. ovifera, the accessions of the different morphotypes were basically grouped according to the fruit colour. This may indicate different times of development and also the extent of breeding in the accessions used. This study has allowed identification of new types that can be employed for the development of new cultivars. The landraces of the spp. ovifera, used as ornamental in Europe, have proved to be of great interest for preserving the diversity of C. pepo.

Cytosolic HSP90 associates with and modulates the Arabidopsis RPM1 disease resistance protein

Abstract: The Arabidopsis protein RPM1 activates disease resistance in response to Pseudomonas syringae proteins targeted to the inside of the host cell via the bacterial type III delivery system. We demonstrate that specific mutations in the ATP-binding domain of a single Arabidopsis cytosolic HSP90 isoform compromise RPM1 function. These mutations do not affect the function of related disease resistance proteins. RPM1 associates with HSP90 in plant cells. The Arabidopsis proteins RAR1 and SGT1 are required for the action of many R proteins, and display some structural similarity to HSP90 co-chaperones. Each associates with HSP90 in plant cells. Our data suggest that (i) RPM1 is an HSP90 client protein; and (ii) RAR1 and SGT1 may function independently as HSP90 cofactors. Dynamic interactions among these proteins can regulate RPM1 stability and function, perhaps similarly to the formation and regulation of animal steroid receptor complexes.

A new chromo-chemodosimeter selective for sulfide anion

Abstract: A new chromo-chemodosimeter for the anion sulfide in aqueous environments has been developed. The recognition concept is based on a pyrylium-thiopyrylium transformation that is conveniently drawn toward "color chemistry" concepts by means of the anchoring of an aniline group. This causes the color of the aniline-pyrylium backbone in L1 to change selectively from magenta to blue when transformed to the corresponding aniline-thiopyrylium in the presence of the sulfide anion. The method is simple and easy, suggesting potential applications in a variety of different environments where easy and rapid determination of sulfide might be required.

An integrated optical interferometric nanodevice based on silicon technology for biosensor applications

Abstract: Integrated optical sensors have become important in recent years since they are the only technology which allows the direct detection of biomolecular interactions. Moreover, silicon microelectronics technology allows mass production as well as the fabrication of nano-/macrosystems on the same platform by hybrid integration of sources, sensors, photodetectors and complementary metal-oxide semiconductor electronics.For the fabrication of an optical sensor nanodevice with an integrated Mach–Zehnder interferometric (MZI) configuration, the optical waveguides must have two main features: monomode behaviour and a high surface sensitivity. In this paper we present the development of a MZI sensor based on total internal reflection waveguides with nanometre dimensions. The aim is to use these sensors in environmental control to detect water pollutants by immunoassay techniques.

Solving the Multi-Mode Resource-Constrained Project Scheduling Problem with genetic algorithms

Abstract: In this paper we consider the Multi-Mode Resource-Constrained Project Scheduling Problem with makespan minimisation as the objective. We have developed new genetic algorithms, extending the representation and operators previously designed for the single-mode version of the problem. Moreover, we have defined a new fitness function for the individuals who are infeasible. We have tested different variants of the algorithm and chosen the best to be compared to different heuristics previously published, using standard sets of instances included in PSPLIB. Results illustrate the good performance of our algorithm.

Wideband double-fed planar monopole antennas

Abstract: Planar monopole antennas with multiple feed points are proposed to improve pattern and impedance bandwidth. A square planar monopole antenna including two feed points and a bevelled variant are designed. These antennas exhibit an excellent performance compared to existing planar monopoles.

Peripheral regions of natural hammerhead ribozymes greatly increase their self-cleavage activity

Abstract: Natural hammerhead ribozymes are mostly found in some viroid and viroid-like RNAs and catalyze their cis cleavage during replication. Hammerheads have been manipulated to act in trans and assumed to have a similar catalytic behavior in this artificial context. However, we show here that two natural cis-acting hammerheads self-cleave much faster than trans-acting derivatives and other reported artificial hammerheads. Moreover, modifications of the peripheral loops 1 and 2 of one of these natural hammerheads induced a >100-fold reduction of the self-cleavage constant, whereas engineering a trans-acting artificial hammerhead into a cis derivative by introducing a loop 1 had no effect. These data show that regions external to the central conserved core of natural hammerheads play a role in catalysis, and suggest the existence of tertiary interactions between these peripheral regions. The interactions, determined by the sequence and size of loops 1 and 2 and most likely of helices I and II, must result from natural selection and should be studied in order to better understand the hammerhead requirements in vivo.

A zeolite with interconnected 8-, 10- and 12-ring pores and its unique catalytic selectivity.

Abstract: One of the major goals in catalysis is to improve the capabilities of microporous materials. This can be accomplished by synthesizing new zeolites with controlled pore architectures. In particular, zeolites containing channels of different sizes in the same structure are desirable. Here, we report a zeolite containing fully interconnected 8-, 10- and 12-membered-ring pores, synthesized by combining the structure-directing effect of the organic 1,5-bis-(methylpyrrolidinium)-pentane and the framework isomorphic substitution of germanium for silicon. Analysis of the crystal structure determined by direct methods from synchrotron powder diffraction data reveals 16 independent tetrahedrally coordinated atoms. This thermally and hydrothermally stable zeolite structure, when synthesized in its acidic form by incorporating aluminium in the framework, presents unique catalytic shape-selectivity effects derived from its particular pore topology.

Demand-side reserve offers in joint energy/reserve electricity markets

Abstract: We propose a market model that includes demand-side reserve offers and where energy and reserve are jointly dispatched. Generators and consumers can submit offers and bids on five distinct products-energy, upspinning reserve, downspinning reserve, and two kinds of standby reserve. The resources are scheduled and dispatched in a joint auction through a mixed-integer optimization program. The extra scheduling flexibility introduced by demand-side reserve offers can lead to significant gains in economic efficiency. Thus, the results suggest that not only do the consumers increase their profits but, in addition, the market power of the generators is reduced.

Synthesis of a new zeolite structure ITQ-24, with intersecting 10- and 12-membered ring pores.

Abstract: A new 10- and 12-membered ring zeolite, named ITQ-24, has been synthesized, and its structure has been solved. It has been found that this zeolite structure is topologically identical to that proposed for the hypothetical polymorph C of the SSZ-33/SSZ-26/CIT-1 family. This new zeolite has been achieved by using a rational approach of introducing Ge in the framework that has a directing effect toward zeolite structures with double-four-membered rings as secondary building units. Notoriously, active catalytic centers, such as Ti and Al, have been incorporated into this new zeolite, demonstrating that it is catalytically active for alkylation of aromatics.

Fractal zone plates.

Abstract: Fractal zone plates (FZPs), i.e., zone plates with a fractal structure, are described. The focusing properties of this new type of zone plate are compared with those of conventional Fresnel zone plates. It is shown that the axial irradiance exhibited by the FZP has self-similarity properties that can be correlated to those of the diffracting aperture.

New Food Drying Technologies - Use of Ultrasound

Abstract: Reducing water availability is one way to preserve food. Water in solid foods is transferred to a fluid, either gas or liquid; during this process both internal and external resistance affect water transfer from the food. As a consequence, any means to reduce those resistances constitute an improvement of the process, and ultrasound appears to be a way to reduce those resistances. Ultrasound are mechanical waves that produce different effects when travelling through a medium. Among others, those related to mass transfer include micro-stirring at the interface, the so called 'sponge effect' and cavitations. Ultrasound has so far been applied to dehydration in solid-gas systems like onion drying. Nevertheless, the difficulties for the propagation in the air have led to the development of specially adapted transducers that have been applied in the drying of carrots. In solid-liquid systems, ultrasound has been used in the treatment of products immersed in hypertonic solutions, either in sugar solutions for fruits like apples or in salt brine in the case of cheese or meat. An increase in mass transfer is achieved if the threshold power value for the product is attained. (A)

Dispersive phonon linewidths: the E2 phonons of ZnO.

Abstract: Phonon linewidths can exhibit a large variation when either pressure or isotopic masses are changed. These effects yield detailed information about the mechanisms responsible for linewidths and lifetimes, e.g., anharmonicity or isotopic disorder. We report Raman measurements of the linewidth of the upper ${\mathrm{E}}_{2}$ phonons of ZnO crystals with several isotopic compositions and their dependence on pressure. Changes by a factor of 12 are observed at a given temperature. Comparison with calculated densities of one-phonon states, responsible for isotope scattering, and of two-phonon states, responsible for anharmonic decay, yields a consistent picture of these phenomena. Isotopic disorder broadening by $7\text{ }\text{ }{\mathrm{c}\mathrm{m}}^{\ensuremath{-}1}$ is found in samples with mixed $^{16}\mathrm{O}--^{18}\mathrm{O}$ content, whereas the anharmonic processes involve decay into sums and differences of two phonons.

Food dehydration and product structure

Abstract: Dehydration of biological material is a controlled effort to preserve the structure or create a new one that serves for functional purposes. In this context, food dehydration is revisited from the perspective of recent advances in food materials science, knowledge from desiccation in Nature, microstructural probing, novel processing technologies and deeper insights into drying mechanisms, among others. The role of structure in dehydrated products appears evident to understand transport mechanisms and to design functional properties. Some approaches and research topics in structure of dehydrated products are presented and discussed.

Influence of particle size on physical and chemical properties of coconut coir dust as container medium

Abstract: The influence of particle size (diameter between 2 mm) on the physical, physico-chemical and chemical properties of a coconut coir dust from Mexico was studied. Particle size significantly affected the physical properties, especially the air–water relationships. Air content increased and water-holding capacity (total and readily available) decreased with increasing particle diameter. The easily-available water content did not reveal a clear pattern of variation, showing the largest value in the fraction 0.125–0.25 mm. Particle size equivalent to 0.5 mm brought about an important and highly significant change in the physical properties studied. Coconut coir dust particle size also affected the physico-chemical and chemical characteristics, but to a lesser degree. Both the electrical conductivity and the available macro- and micro-element concentrations were significantly higher in the smaller size ranges, the largest values being obtained in the finest particle size fraction (<0.125 mm).