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Institution

Rio de Janeiro State University

EducationRio de Janeiro, Brazil
About: Rio de Janeiro State University is a education organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Population & Large Hadron Collider. The organization has 16631 authors who have published 30919 publications receiving 465753 citations. The organization is also known as: UERJ & Rio de Janeiro State University.


Papers
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Journal ArticleDOI
TL;DR: Results demonstrate that Pseudomonas aeruginosa adherence is related to the dedifferentiation of airway epithelium during the repair process which unmasks and upregulates the alpha5beta1 integrin expression and induces active synthesis of cellular fibronectin.
Abstract: Initial infection of the airway by Pseudomonas aeruginosa may occur through a variety of bacterial strategies including binding to epithelial receptors present at the surface of the respiratory epithelium. In order to characterize the adherence sites for P. aeruginosa in damaged and repairing bronchial tissue, an ex vivo model of airway epithelial injury and repair was developed using primary cell cultures of nasal cells from 14 subjects with polyposis. P. aeruginosa strongly adhered to flattened dedifferentiated (FD) bronchial and nasal cytokeratin 13-positive epithelial cells in the process of migration for repair. In in vitro experiments, competitive binding inhibition assays demonstrated that alpha5beta1 integrins and cellular fibronectin, in particular the RGD sequence, are receptors involved in P. aeruginosa adherence to FD nasal epithelial cells. Fluorescent cell sorting analysis and immunofluorescence techniques revealed that the alpha5beta1 integrins are overexpressed and apically exposed in FD nasal epithelial cells. One 50 kDa outer membrane protein was identified in piliated and nonpiliated strains of P. aeruginosa that was involved in binding to cellular fibronectin and alpha5beta1 epithelial integrins. These results demonstrate that Pseudomonas aeruginosa adherence is related to the dedifferentiation of airway epithelium during the repair process which unmasks and upregulates the alpha5beta1 integrin expression and induces active synthesis of cellular fibronectin. These epithelial receptors are then used by a Pseudomonas aeruginosa 50 kDa outer membrane protein as sites of bacterial adherence.

95 citations

Journal ArticleDOI
TL;DR: The data highlight the fact that a pronounced correlation between genetic and geographic/cultural structure can only be expected under very specific conditions, most of which are likely not to have been met by the ancestors of native South Americans.
Abstract: Numerous studies of human populations in Europe and Asia have revealed a concordance between their extant genetic structure and the prevailing regional pattern of geography and language. For native South Americans, however, such evidence has been lacking so far. Therefore, we examined the relationship between Y-chromosomal genotype on the one hand, and male geographic origin and linguistic affiliation on the other, in the largest study of South American natives to date in terms of sampled individuals and populations. A total of 1,011 individuals, representing 50 tribal populations from 81 settlements, were genotyped for up to 17 short tandem repeat (STR) markers and 16 single nucleotide polymorphisms (Y-SNPs), the latter resolving phylogenetic lineages Q and C. Virtually no structure became apparent for the extant Y-chromosomal genetic variation of South American males that could sensibly be related to their inter-tribal geographic and linguistic relationships. This continent-wide decoupling is consistent with a rapid peopling of the continent followed by long periods of isolation in small groups. Furthermore, for the first time, we identified a distinct geographical cluster of Y-SNP lineages C-M217 (C3*) in South America. Such haplotypes are virtually absent from North and Central America, but occur at high frequency in Asia. Together with the locally confined Y-STR autocorrelation observed in our study as a whole, the available data therefore suggest a late introduction of C3* into South America no more than 6,000 years ago, perhaps via coastal or trans-Pacific routes. Extensive simulations revealed that the observed lack of haplogroup C3* among extant North and Central American natives is only compatible with low levels of migration between the ancestor populations of C3* carriers and non-carriers. In summary, our data highlight the fact that a pronounced correlation between genetic and geographic/cultural structure can only be expected under very specific conditions, most of which are likely not to have been met by the ancestors of native South Americans.

95 citations

Journal ArticleDOI
TL;DR: It is concluded that the pig kidney is a good animal model for endourologic research and training because of the anatomic findings on collecting system anatomy and renal morphometry in the pig.
Abstract: We present detailed anatomic findings on collecting system anatomy and renal morphometry in the pig and compare these findings with previous findings in humans. We studied three-dimensional polyester resin corrosion endocasts of the pelviocaliceal system obtained from 100 kidneys (50 pigs). Eighty kidneys were evaluated morphometrically, considering length, cranial pole width, caudal pole width, thickness, and weight. The pig collecting system was classified into two major groups (A and B). Group A (40%) was composed of kidneys in which the mid-zone is drained by calices dependent on the cranial or the caudal caliceal group or both. Group B (60%) kidneys have the mid-zone drained by calices independent of the polar groups. Group B includes two subtypes (B-I and B-II). The pig collecting system showed only angles smaller than 90 degrees between the caudal (lower) infundibulum and the renal pelvis. Renal morphometric measurements revealed the following means: length 11.8 cm, cranial pole width 5.64 cm, caudal pole width 5.35 cm, thickness 2.76 cm, and weight 98 g. As in human kidneys, one may group the pig collecting system into two groups. Nevertheless, in pigs, we did not find a subdivision of Group A. The incidence of collecting systems in Groups A and B and the subtypes of Group B in pigs are different from those in humans. Also different from humans, in pigs, we found only angles smaller than 90 degrees between the caudal (lower) infundibulum and the renal pelvis. Except for the length, the means of the other morphometric measurements of the pig kidney are smaller than those of humans. From an anatomic standpoint, despite the differences pointed out, we conclude that the pig kidney is a good animal model for endourologic research and training.

95 citations

Proceedings ArticleDOI
14 May 2016
TL;DR: The results of a qualitative study exploring requirements engineering practitioners’ perceptions and attitudes towards sustainability suggest the need for focused efforts in sustainability education, but also a need to rethink professional norms and practices.
Abstract: Sustainability is now a major concern in society, but there is little understanding of how it is perceived by software engineering professionals and how sustainability design can become an embedded part of software engineering process. This paper presents the results of a qualitative study exploring requirements engineering practitioners' perceptions and attitudes towards sustainability. It identifies obstacles and mitigation strategies regarding the application of sustainability design principles in daily work life. The results of this study reveal several factors that can prevent sustainability design from becoming a first class citizen in software engineering: software practitioners tend to have a narrow understanding of the concept of sustainability; organizations show limited awareness of its potential opportunities and benefits; and the norms in the discipline are not conducive to sustainable outcomes. These findings suggest the need for focused efforts in sustainability education, but also a need to rethink professional norms and practices.

95 citations

Journal ArticleDOI
TL;DR: It is argued that cross-platform analysis addresses a gap in research methods in that it acknowledges the interplay between a social phenomenon under investigation and the medium within which it is being researched, thus illuminating the different affordances and cultures of web platforms.
Abstract: Analysis of social media using digital methods is a flourishing approach. However, the relatively easy availability of data collected via platform application programming interfaces has arguably le...

95 citations


Authors

Showing all 16818 results

NameH-indexPapersCitations
Hyun-Chul Kim1764076183227
Maria Elena Pol139141499240
Wagner Carvalho135139594184
Alberto Santoro1351576100629
Andre Sznajder134146498242
Luiz Mundim133141389792
Helio Nogima132127484368
D. De Jesus Damiao128116282707
Magdalena Malek12859867486
Sudha Ahuja127101675739
Helena Malbouisson125115182692
Jose Chinellato123111664267
Flavia De Almeida Dias12059059083
Gilvan Alves11982969382
C. De Oliveira Martins11988066744
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20241
202362
2022281
20212,251
20202,453
20192,072