Romanian Academy

About: Romanian Academy is a archive organization based out in Bucharest, Romania. It is known for research contribution in the topics: Population & Nonlinear system. The organization has 3662 authors who have published 10491 publications receiving 146447 citations. The organization is also known as: Academia Română & Societatea Literară Română.

High-performance separation techniques hyphenated to mass spectrometry for ganglioside analysis†

Abstract: Gangliosides, sialic-acid-containing glycosphingolipids are involved in numerous biological processes and play essential roles in severe pathologies, with predilection in those of the central nervous system. Formerly, ganglioside composition and quantity were assessed exclusively by thin-layer chromatographic (TLC), immunochemical, and immunohistochemical methods, which have limited effectiveness being unable to detect minor components in mixtures of high heterogeneity. Increased awareness of the biological importance of gangliosides stimulated the development of analytical methods that are better amenable to complex ganglioside mixtures. More recently, MS in online conjunction with high-performance separation techniques brought a significant progress to the field. This review highlights the state-of-the-art development and application of separation methods online coupled to MS for ganglioside analysis. Most original and successful protocols based on GC-MS, LC-MS, and CE-MS are presented here together with the special instrumental and sample preparation requirements to be met for effective ganglioside separation, detection, and structural identification. Finally, the advantages and downsides of each methodology as well as the perspectives for simplification, standardization, and upgrading are assessed.

Foam cell‐derived 4‐hydroxynonenal induces endothelial cell senescence in a TXNIP‐dependent manner

Abstract: Vascular endothelial cell (VEC) senescence is considered an early event in the development of atherosclerotic lesions. Stressful stimuli, in particular oxidative stress, have been linked to premature senescence in the vasculature. Foam cells are a major source of reactive oxygen species and may play a role in the induction of VEC senescence; hence, we investigated their involvement in the induction of VEC senescence in a co-culture transwell system. Primary bovine aortic endothelial cells, exposed to the secretome of THP-1 monocyte-derived foam cells, were analysed for the induction of senescence. Senescence associated β-galactosidase activity and the expression of p16 and p21 were increased, whereas phosphorylated retinoblastoma protein was reduced. This senescent phenotype was mediated by 4-hydroxnonenal (4-HNE), a lipid peroxidation product secreted from foam cells; scavenging of 4-HNE in the co-culture medium blunted this effect. Furthermore, both foam cells and 4-HNE increased the expression of the pro-oxidant thioredoxin-interacting protein (TXNIP). Molecular manipulation of TXNIP expression confirmed its involvement in foam cell-induced senescence. Previous studies showed that peroxisome proliferator-activated receptor (PPAR)δ was activated by 4-hydroalkenals, such as 4-HNE. Pharmacological interventions supported the involvement of the 4-HNE-PPARδ axis in the induction of TXNIP and VEC senescence. The association of TXNIP with VEC senescence was further supported by immunofluorescent staining of human carotid plaques in which the expression of both TXNIP and p21 was augmented in endothelial cells. Collectively, these findings suggest that foam cell-released 4-HNE activates PPARδ in VEC, leading to increased TXNIP expression and consequently to senescence.

Prototyping a Ferrofluid-Cooled Transformer

Abstract: This paper presents the work conducted on prototyping a step-up/step-down, single-phased, low-power, and medium-voltage electrical transformer cooled by a fluid with colloidal magnetic nanoparticles. The magnetic and fluid dynamic properties and the heat capacities of the ferrofluid (magnetic nanofluid) and that of the regular coolant (UTR-40 transformer oil) were experimentally determined and comparatively evaluated. Mathematical models for the electromagnetic field and the heat transfer were defined and numerically solved to assess the capacity of the transformer to sustain the working conditions. The simulation results were utilized to improve the design of a prototype, where the UTR-40 regular coolant is replaced by ferrofluid. The numerical simulation results and the experiments evidence the superior performance of the prototype.

Resonant (p, 2)-equations with asymmetric reaction

Abstract: We consider a nonlinear, nonhomogeneous Dirichlet problem driven by the sum of a p-Laplacian and a Laplacian, 2 < p < ∞ ((p, 2)-equation) and with a reaction which exhibits asymmetric behavior at +∞ and at -∞ and is resonant. Using variational methods together with Morse theoretic arguments, we prove the existence of two and three nontrivial solutions.