Institution
Romanian Academy
Archive•Bucharest, Romania•
About: Romanian Academy is a archive organization based out in Bucharest, Romania. It is known for research contribution in the topics: Population & Nonlinear system. The organization has 3662 authors who have published 10491 publications receiving 146447 citations. The organization is also known as: Academia Română & Societatea Literară Română.
Papers published on a yearly basis
Papers
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TL;DR: In this article, the properties of a quaternary Ti-20Nb-10Zr-5Ta alloy were investigated in relation to its use in biomedical applications and its microstructure was revealed by X-ray diffraction, and composition by energy dispersive Xray measurements.
92 citations
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TL;DR: The molecular mechanism of oestrogen‐agonistic/antagonistic action of structurally similar ligands, bisphenol (BP) and bispenol A (BPA) on cell proliferation and apoptosis of ERα + ve breast cancer cells is determined.
Abstract: Background and Purpose
Oestrogen receptor alpha (ERα) binds to different ligand which can function as complete/partial oestrogen-agonist or antagonist. This depends on the chemical structure of the ligands which modulates the transcriptional activity of the oestrogen-responsive genes by altering the conformation of the liganded-ERα complex. This study determined the molecular mechanism of oestrogen-agonistic/antagonistic action of structurally similar ligands, bisphenol (BP) and bisphenol A (BPA) on cell proliferation and apoptosis of ERα + ve breast cancer cells.
Experimental Approach
DNA was measured to assess the proliferation and apoptosis of breast cancer cells. RT-PCR and ChIP assays were performed to quantify the transcripts of TFF1 gene and recruitment of ERα and SRC3 at the promoter of TFF1 gene respectively. Molecular docking was used to delineate the binding modes of BP and BPA with the ERα. PCR-based arrays were used to study the regulation of the apoptotic genes.
Key Results
BP and BPA induced the proliferation of breast cancer cells; however, unlike BPA, BP failed to induce apoptosis. BPA consistently acted as an agonist in our studies but BP exhibited mixed agonistic/antagonistic properties. Molecular docking revealed agonistic and antagonistic mode of binding for BPA and BP respectively. BPA treatment resembled E2 treatment in terms of PCR-based regulation of apoptotic genes whereas BP was similar to 4OHT treatment.
Conclusions and Implications
The chemical structure of ERα ligand determines the agonistic or antagonistic biological responses by the virtue of their binding mode, conformation of the liganded-ERα complex and the context of the cellular function.
92 citations
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TL;DR: The universality of P systems with active membranes which are allowed to change the labels of membranes, but do not use polarizations is obtained, and it is proved that SAT can be solved in linear time by systems without polarizations and with label changing possibilities.
Abstract: This paper addresses the problem of removing the polarization of membranes from P systems with active membranes - and this is achieved by allowing the change of membrane labels by means of communication rules or by membrane dividing rules. As consequences of these results, we obtain the universality of P systems with active membranes which are allowed to change the labels of membranes, but do not use polarizations. Universality results are easily obtained also by direct proofs. By direct constructions, we also prove that SAT can be solved in linear time by systems without polarizations and with label changing possibilities. If non-elementary membranes can be divided, then SAT can be solved in linear time without using polarizations and label changing. Several open problems are also formulated.
92 citations
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TL;DR: It is demonstrated that the sialic acids of membrane glycoconjugates control the life span of erythrocytes and that the desialylation of glycans is responsible for the clearance of the aged ery Throcytes.
Abstract: Comparing the properties of 'young' and senescent ('aged') O+ erythrocytes isolated by applying ultracentrifugation in a self-forming Percoll gradient, we demonstrate that the sialic acids of membrane glycoconjugates control the life span of erythrocytes and that the desialylation of glycans is responsible for the clearance of the aged erythrocytes. This capture is mediated by a beta-galactolectin present in the membrane of macrophages. The evidence supporting these conclusions is as follows: (1) Analysis by flow cytofluorimetry of the binding of fluorescein isothiocyanate labelled lectins specific for sialic acids shows that the aged erythrocytes bind less WGA, LPA, SNA and MAA than young erythrocytes. The binding of DSA and LCA is not modified. On the contrary, the number of binding sites of UEA-I specific for O antigen and of AAA decreases significantly. PNA and GNA do not bind to erythrocytes. (2) RCA120 as well as Erythrina cristagalli and Erythrina corallodendron lectins specific for terminal beta-galactose residues lead to unexpected and unexplained results with a decrease in the number of lectin binding sites associated with increasing desialylation. (3) The glycoconjugates from the old erythrocytes incorporate more sialic acid than the young cells. This observation results from the determination of the rate of transfer by alpha-2,6-sialyltransferase of fluorescent or radioactive N-acetylneuraminic acid, using as donors CMP-9-fluoresceinyl-NeuAc and CMP-[14C]-NeuAc, respectively. (4) Microscopy shows that the old erythrocytes are captured preferentially by the macrophages relative to the young ones. Fixation of erythrocytes by the macrophage membrane is inhibited by lactose, thus demonstrating the involvement of a terminal beta-galactose specific macrophage lectin. (5) Comparative study of the binding of WGA, LPA, SNA and MAA to the aged erythrocytes and to the in vitro enzymatically desialylated erythrocytes shows that the desialylation rate of aged cells is low but sufficient to lead to their capture by the macrophages.
92 citations
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TL;DR: This milestone report describes the main problems, such as management of complexity, scalability, increasing costs, coordination, market-based resource allocation, and more in the next generation manufacturing system.
91 citations
Authors
Showing all 3740 results
Name | H-index | Papers | Citations |
---|---|---|---|
Cristina Popescu | 74 | 285 | 18434 |
Adrian Covic | 73 | 570 | 17379 |
Gheorghe Paun | 65 | 399 | 18513 |
Floriana Tuna | 60 | 271 | 11968 |
Arto Salomaa | 56 | 374 | 17706 |
Jan A. Bergstra | 55 | 616 | 13436 |
Alexandru T. Balaban | 53 | 605 | 14225 |
Cristian Sminchisescu | 53 | 173 | 12268 |
Maya Simionescu | 47 | 192 | 10608 |
Marius Andruh | 46 | 239 | 8431 |
Werner Scheid | 46 | 518 | 9186 |
Vicenţiu D. Rădulescu | 46 | 360 | 7771 |
Cornelia Vasile | 44 | 297 | 7108 |
Irinel Popescu | 44 | 401 | 8448 |
Mihail Barboiu | 44 | 239 | 5789 |