Showing papers by "Rovira i Virgili University published in 1993"
TL;DR: Manganese (II) chloride tetrahydrate was investigated in Swiss mice for maternal and developmental toxicity after subcutaneous exposure to doses of 0, 2, 4, 8 and 16 mg/kg per day from gestation day 6 through 15, with no differences between control and manganese-treated groups in the incidence of individual or total malformations.
65 citations
TL;DR: Therefore, vanadium would not cause any adverse effect on fertility or testicular function in male mice at the concentrations usually ingested by humans through the diet and drinking water.
48 citations
TL;DR: The ability of Tiron to protect the developing mouse fetus against uranium-induced developmental toxicity offers only modest encouragement with regard to its possible therapeutic potential for pregnant women exposed to this metal.
28 citations
TL;DR: 2 patients with minimal-change glomerulopathy associated with an undifferentiated carcinoma of unknown origin and urothelial carcinoma are described and MCG should be included in the nephropathies which cause nephrotic syndrome in adult patients with carcinoma.
Abstract: We describe 2 patients with minimal-change glomerulopathy (MCG) associated with an undifferentiated carcinoma of unknown origin and urothelial carcinoma. Oliguric acute renal failure and histopathological changes consistent with acute tubular necrosis were also observed. Fourteen other cases of MCG complicating solid tumors reported in the literature are reviewed. MCG should be included in the nephropathies which cause nephrotic syndrome in adult patients with carcinoma.
17 citations
Journal Article•
TL;DR: Gestational day 12 is the most sensitive time for metavanadate-induced developmental toxicity in mice, with a significant decrease in the fetal body weight/litter.
Abstract: The aim of this study was to assess the variability in the developmental toxicity of vanadate with the day of administration during gestation. Single ip injections of 25 mg sodium metavanadata/kg were given to albino Swiss mice on one of the days 9-12 of gestation. Dams were killed on day 18 of pregnancy, and fetuses were examined for external, internal and skeletal malformations and variations. The number of dead or resorbed fetuses/litter, as well as the percentage of postimplantation loss, were significantly increased with injections on days 9-12 of gestation. However, the most sensitive time for the induction of metavanadate embryotoxicity was gestational day 12. Metavanadate treatment on day 12, but not days 9-11, resulted in a significant decrease in the fetal body weight/litter. There were no external, internal or skeletal malformations, whereas the most common skeletal variations were a reduced ossification in the parietal bone, metatarsals and metacarpals, bipartite sternebrae and fused ribs. The highest percentage of total skeletal defects was found on day 12 (82.3%). Gestational day 12 is the most sensitive time for metavanadate-induced developmental toxicity in mice.
14 citations
TL;DR: Tiron offers encouragement with regard to its therapeutic potential for pregnant women exposed to vanadate, however, further investigations, including the effect of increasing the time interval between acuteVanadate exposure and initiation of Tiron therapy, are required.
Abstract: Vanadate is embryotoxic and fetotoxic in golden hamsters, mice and rats. Tiron (sodium 4,5-dihydroxybenzene-1,3-disulfonate), a chelating agent widely used in analytical chemistry, is an effective antidote in the treatment of oral or parenteral vanadate poisoning. The present study evaluated the effect of administration of Tiron on sodium metavanadate (NaVO3)-induced developmental toxicity in mice. NaVO3 (25 mg/kg, i.p.) was injected on day 12 of gestation, whereas Tiron was injected subcutaneously at 0, 24, 48, and 72 hr after NaVO3 administration. Tiron effectiveness was assessed at dosage levels of 0, 250, 500, and 1,000 mg/kg. Cesarean sections were performed on gestation day 18. All live fetuses were examined for external, internal, and skeletal malformations and variations. Amelioration by Tiron of NaVO3 developmental toxicity was evidenced by a significant decrease in the number of resorbed fetuses, an increase in the mean fetal weight, and a reduction in the incidence of the skeletal variations caused by NaVO3. According to these results, Tiron offers encouragement with regard to its therapeutic potential for pregnant women exposed to vanadate. However, further investigations, including the effect of increasing the time interval between acute vanadate exposure and initiation of Tiron therapy, are required.
14 citations
TL;DR: Fertility and reproductive performance in the gallium nitrate-treated groups did not differ significantly from controls, and no significant changes were observed in the relative testes and epididymis weights.
8 citations
TL;DR: It is concluded that the octanol-water system may be useful screening compounds with strontium chelation potential, since in previous in vivo studies both compounds were also effective in the removal ofstrontium following internal contamination.
Abstract: 90Sr has a physical half-life of sufficient duration to make it a potentially dangerous contaminant from nuclear accidents and radioactive wastes. In the present study, the efficacy of 16 compounds as potential chelators of strontium was tested in vitro. Strontium solubilization from strontium carbonate and its distribution in an octanol-water system (Do/w) was determined in the absence and presence of alpha-ketoglutaric acid, Kryptofix 222, ethylenglycol-bis-(beta-amino-ethylether)-N,N-tetraacetic acid, diethylentriamine pentaacetic acid, Kryptofix 5, disodium chlodronate, disodium ethidronate, oxaloacetic acid, fumaric acid, D-gluconic acid, succinic acid, citric acid, D,L-2,3-diaminopropionic acid, 1,1-cyclohexanediacetic acid, tartaric acid, and trans-1,2-cy-clohexanediol. Kryptofix 222 and Kryptofix 5 significantly increased solubilized strontium, suggesting strontium chelation potential. Since in previous in vivo studies both compounds were also effective in the removal of strontium following internal contamination, it is concluded that the octanol-water system may be useful screening compounds with strontium chelation potential.
5 citations
Journal Article•
TL;DR: Both enzymatic forms showed essentially the same temperature- and pH-activity patterns, however, the pH stability of the CAS-enzyme complex at pH 6 was significantly higher than the beta-glucosidase in free solution.
Abstract: Lyophylized almond beta-glucosidase (EC 3.2.1.21) has been adsorbed onto Concanavalin A-Sepharose (CAS). The yield of the enzymatic units of the CAS-enzyme complex preparation was 131%. The values of the kinetic parameters of the free beta-glucosidase were: kM = 1.7 mM and Vmax = 330.1 U/mg protein. The immobilised form showed the following values: kM = 1.7 mM and Vmax = 402.6 U/mg protein. Both enzymatic forms showed essentially the same temperature- and pH-activity patterns (temperature optimum: 50 degrees C and pH optimum approx. 6.0), however, the pH stability of the CAS-enzyme complex at pH 6 was significantly higher than the beta-glucosidase in free solution.
5 citations
TL;DR: In this article, a Spanish version of the Reducer-augmenter Scale (RAS) is presented, which is based on an initial pool of items taken from Vando's RAS and from Clapper's RRAS revision.
4 citations