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Showing papers by "Russian State Agricultural University published in 2009"


Journal ArticleDOI
TL;DR: It was shown that the presence of just a single gene of serine proteinase inhibitor provides sufficient protection at least against two bacterial phytopathogens, Pseudomonas syringae pv.
Abstract: The possibility to use agrobacterial transformation of leaf discs to produce resistance to bacterial infections in tobacco and potato plants by introduction of a single gene encoding the serine proteinase inhibitor BWI-1a (ISP) from buckwheat seeds is shown. All studied PCR-positive transgenic plants exhibited antibacterial activity in biotests. It was shown that the presence of just a single gene of serine proteinase inhibitor provides sufficient protection at least against two bacterial phytopathogens, Pseudomonas syringae pv. tomato and Clavibacter michiganensis sbsp. michiganensis. The biotest including tobacco plant infection by the white wings butterfly in the green house has also demonstrated the existence of protective effect in transgenic tobacco plants. Significant genotypic variations in the protection efficiency were found between members of different genera of the same family (potato and tobacco) as well as between different lines of the same species. Northern blot analysis of four transgenic potato lines and three tobacco lines transformed by a vector plasmid containing the ISP gene of serine proteinases BWI-1a from buckwheat seeds has shown the presence of the expected size mRNA transcript.

33 citations


Journal ArticleDOI
TL;DR: The GISH technology was adapted for woody ornamentals, commonly characterized by small genomes and/or high amounts of small chromosomes, and successful to label entire chromosomes by using an optimal probe/block ratio and the best probe labelling and detection system.
Abstract: With 2 figures and 1 table Abstract Genomic in situ hybridization (GISH) is a powerful tool in distinguishing parental genomes in plant hybrids. However, in plants with small genome GISH often failed to decorate entire chromosomes. In this study, the GISH technology was adapted for woody ornamentals, commonly characterized by small genomes (between 842.8 and 2430.4 Mbp/1C or 0.86 and 2.48 pg/1C) and/or high amounts of small chromosomes (up to 82 chromosomes with size 1.4–4.8 μm). The used GISH method was successful to label entire chromosomes by using an optimal probe/block ratio (>1/80) and the best probe labelling and detection system (biotin vs. digoxigenin). GISH was performed on Buddleja and Hibiscus hybrids resulting from an interspecific breeding programme. For the first time, GISH on Buddleja × weyeriana‘Sungold’ (F2 of B. globosa × B. davidii) showed a chromosomal origin of the hybrid: 36 chromosomes inherited from B. davidii (2n = 2x = 76), 28 chromosomes from B. globosa (2n = 2x = 38) and 12 chromosomes were recombinant chromosomes between B. globosa and B. davidii. The detected chromosome constitution in B. x weyeriana points on 2n-gametes formation during meiosis of B. globosa. GISH analysis of F1 and F2 hybrids of B. davidii×B. × weyeriana crosses revealed that 16 (F1) and 10 (F2) chromosomes completely belong to B. davidii and all the other chromosomes were recombinant. This proved that all chromatin material of B. globosa was introgressed into the B. davidii chromosomes. GISH analysis of an F1 hybrid between Hibiscus syriacus × H. paramutabilis revealed 40 chromosomes of H. syriacus (2n = 4x = 80) and 41 chromosomes of H. paramutabilis (2n = 4x = 82). In an F2 hybrid of H. syriacus ×H. paramutabilis 25 recombinant chromosomes were detected, indicating introgression of H. syriacus DNA in H. paramutabilis.

16 citations


Journal ArticleDOI
TL;DR: The novel lipid-transporting protein Ns-LTP1, isolated from seeds of the garden fennel flower Nigella sativa, is capable of suppressing the development of some phytopathogenic fungi and oomycetes.
Abstract: A novel lipid-transporting protein (Ns-LTP1) has been isolated from seeds of the garden fennel flower Nigella sativa. The molecular mass, N-terminal amino acid sequence, and amino acid composition of the protein have been determined. Ns-LTP1 has a molecular mass of 9602 Da and contains eight cysteine residues which form four disulfide bridges. The protein is capable of suppressing the development of some phytopathogenic fungi and oomycetes.

8 citations