Showing papers by "Saint Francis University published in 1980"

Stimulation of DNA and collagen synthesis by autologous growth factor in cultured fetal rat calvaria.

Abstract: Conditioned medium derived from organ or cell cultures prepared from 19- to 21-day fetal rat calvaria stimulated the incorporation of [3H]proline collagen and of [3H]thymidine into DNA in organ cultures of the same tissue. Addition of cortisol enhanced the effect on collagen but not on DNA synthesis. These effects appeared to be due to a nondialyzable and heat-stable growth factor.

Effect of fibroblast growth factor on cultured fetal rat calvaria.

Abstract: Normal rat or human serum causes a greater incorporation of 3H-proline into bone collagenase digestible protein (CDP) and noncollagen protein (NCP) than does serum from hypophysectomized animals or hypopituitary humans. In the present study, we have tested fibroblast growth factor (FGF), a peptide isolated from bovine pituitary glands that has been shown to stimulate RNA and DNA synthesis in various mesodermal cells, for its effects on cultured fetal rat calvaria. The major effect of FGF appeared to be a stimulation of periosteal fibroblastic cell proliferation. Incorporation of 3H-thymidine into DNA was increased at concentrations of 10–1000 ng/ml; the effect appeared after 12 hr, was sustained for 96 hr, and could not be ascribed to an effect on 3H-thymidine uptake. Total DNA content was increased and histologic sections showed an increase in the number of mitoses in periosteal fibroblasts after colcemid arrest. These effects were accompanied by an increase in the uptake and incorporation of 3H-uridine, a decrease in the incorporation of labeled proline into CDP, and a small and variable increase in the incorporation of proline into NCP. Cortisol opposed the effects of FGF on 3H-thymidine and 3H-uridine incorporation. Insulin did not alter the effect of FGF on 3H-thymidine incorporation, but FGF decreased the stimulatory effect of insulin on the labeling of CDP. The effect of FGF on thymidine incorporation and collagen synthesis was not altered by indomethacin. The major effect of FGF in calvaria is to increase DNA synthesis and stimulate the proliferation of periosteal fibroblasts. It does not appear to be the pituitary-dependent factor in serum that stimulates 3H-proline incorporation into CDP and NCP in calvaria.

Hyperparathyroidism in the elderly.

Abstract: Primary hyperparathyroidism seems to be increasing in incidence in elderly patients. Their symptoms are somewhat different from those encountered in younger patients, the most striking difference being the increase in the number of patients with mental changes. Twenty-nine patients, 23 women and 6 men, were treated at two community hospitals. Surgery was successful in all instances with no postoperative mortality and a low rate of complications. When properly evaluated, elderly patients tolerate parathyroid exploration very satisfactorily.

Relationship between Patients' In-Process Evaluations of Therapy and Psychotherapy Outcome.

Abstract: Investigated relationship between patients' session-by-session evaluations of brief psychotherapy and outcome. Twenty-eight patients were seen by six therapists for a mean of 10 sessions in a university health service setting. In addition to the session evaluations, which were administered at the conclusion of the second session and each session thereafter, a variety of dependent measures were administered pre and post. Factor analysis of these dependent measures yielded three factors, which were correlated with session evaluation scores. Results indicated that patients' in-process evaluations were correlated significantly and positively with some positive treatment outcome measures, especially achievement of patients' specified treatment goals, but only during initial and final phases of therapy. Results were interpreted as supportive of Mann's model of brief psychotherapy.

Listeria monocytogenes empyema and bacteremia complicating chronic lymphocytic leukemia.

Abstract: A 60-year-old man receiving corticosteroid and cyclophosphamide therapy for chronic lymphocytic leukemia developed an empyema and bacteremia due to Listeria monocytogenes. The rapidly fulminant course of Listeria infections in immunosuppressed hosts and the necessity of prompt treatment of Listeria empyemas are emphasized. Cancer 46:619–620, 1980.