Showing papers by "Saint Francis University published in 1981"

Effect of platelet-derived growth factor on DNA and protein synthesis in cultured rat calvaria

Abstract: Platelet-derived growth factor (PDGF), a peptide isolated from human platelets, was studied for its effects on bone formation by examining the synthesis of DNA, collagen and noncollagen protein in cultures of 21 day fetal rat calvaria. PDGF, at concentrations of 0.6-200 ng/ml, increased the incorporation of 3H-thymidine into acid insoluble residues in calvaria cultured for 24-96 hr; PDGF also increased the bone DNA content. After 24 hr of culture, PDGF caused a dose-dependent stimulation of 3H-proline incorporation into noncollagen protein (NCP) and a small stimulation of its incorporation into collagenase-digestible protein (CDP). After 96 hr of culture, PDGF did not affect NCP but inhibited the labeling of CDP. The PDGF stimulatory effect on the incorporation of 3H-thymidine was seen in both the periosteum and periosteum-free calvarium while that on the labeling of NCP was seen in the central nonperiosteal bone. Histological sections showed a threefold increase in the mitotic index after colcemid arrest in PDGF-treated bones. The stimulatory effect of PDGF on the incorporation of 3H-thymidine was enhanced by cortisol. These studies indicate that PDGF stimulates bone DNA synthesis and has a no specific stimulatory effect on protein synthesis in short-term cultures.

The teaching of nursing research: a survey report.

Abstract: How healthy is the research teaching at your school? This article presents survey results that offer detailed information regarding the status of research teaching at schools of nursing throughout the United States. The data provide a basis for individual program evaluations as well as for assessment of the overall development of nursing research. This is the first in a series of three articles that focus on the teaching of nursing research.

Glycosylated hemoglobin in patients treated by chronic dialysis.

Abstract: A glycosylated hemoglobin (HbA1) test was used to evaluate the role of dialysate glucose in the development of carbohydrate intolerance and hyperlipidemia in chronic hemodialysis patients and chronic peritoneal dialysis patients. HbA1 levels were significantly elevated in all groups of patients. HbA1 levels were not ameliorated with 8 weeks of glucose-free hemodialysis. There was no correlation between HbA1 and serum glucose, triglycerides, or cholesterol. Thus, HbA1 elevation cannot be explained solely by glucose reabsorption from dialysate. This test is helpful in the detection of carbohydrate intolerance, but its usefulness in evaluation of hyperlipidemia of dialysis patients is uncertain.