Showing papers by "Saskatchewan Health published in 1998"
TL;DR: Evidence is provided that Luman and VP16 share two important structural features: an acidic activation domain and a common mechanism for binding HCF that suggest that the HCF-Luman interaction may represent a conserved mechanism for transcriptional regulation in metazoans.
Abstract: In human cells infected with herpes simplex virus (HSV), viral gene expression is initiated by the virion protein VP16. VP16 does not bind DNA directly but forms a multiprotein complex on the viral immediate-early gene promoters with two cellular proteins: the POU domain protein Oct-1 and host cell factor (HCF; also called C1, VCAF, and CFF). Despite its apparent role in stabilizing the VP16-induced transcription complex, the natural biological role of HCF is unclear. Only recently HCF has been implicated in control of the cell cycle. To determine the role of HCF in cells and answer why HSV has evolved an HCF-dependent mechanism for the initiation of the lytic cycle, we identified the first human ligand for HCF (R. Lu et al., Mol. Cell. Biol. 17:5117–5126, 1997). This protein, Luman, is a member of the CREB/ATF family of transcription factors that can activate transcription from promoters containing cyclic AMP response elements (CRE). Here we provide evidence that Luman and VP16 share two important structural features: an acidic activation domain and a common mechanism for binding HCF. We found that Luman, its homolog in Drosophila, dCREB-A (also known as BBF-2), and VP16 bind to HCF by a motif, (D/E)HXY(S/A), present in all three proteins. In addition, a mutation (P134S) in HCF that prevents VP16 binding also abolishes its binding to Luman and dCREB-A. We also show that while interaction with HCF is not required for the ability of Luman to activate transcription when tethered to the GAL4 promoter, it appears to be essential for Luman to activate transcription through CRE sites. These data suggest that the HCF-Luman interaction may represent a conserved mechanism for transcriptional regulation in metazoans, and HSV mimics this interaction with HCF to monitor the physiological state of the host cell.
103 citations
TL;DR: A population-based study to estimate the incidence of hospitalizations for aplastic anemia and agranulocytosis and to evaluate associations between these disorders and antecedent prescription drug use was performed using the Saskatchewan administrative health care databases.
45 citations
TL;DR: The intact insecticide was found in the post-mortem blood and gastric contents at concentrations of 1.8 and 978 micrograms/ml, respectively.
28 citations
TL;DR: There were no apparent trends for the levels of any metals versus age nor were there any differences in average hepatic metal concentrations for males and females.
Abstract: Autopsied liver tissue samples collected from 42 males and 31 females were analyzed for copper, manganese and zinc using atomic absorption spectrometry (AAS). With the exception of two liver samples for which the copper levels were determined to be 74.8 and 104.0 μg/g (dry weight), hepatic copper concentrations were found to range from 1.7 to 32.4 μg/g with a mean concentration of 14.2 μg/g and standard deviation of 7.0 μg/g. Manganese concentrations (with the exception of one sample having 12.9 μg/g) ranged from 0.22 to 4.6 μg/g with a mean of 2.26 ± 1.00 μg/g. Hepatic zinc levels averaged 118.3 ± 44.4 μg/g and ranged from 38.5 to 231.3 μg/g. There were no apparent trends for the levels of any metals versus age nor were there any differences in average hepatic metal concentrations for males and females. © Rapid Science 1998.
25 citations
TL;DR: An analytical procedure was developed in which thirteen herbicides may be extracted from drinking water samples using solid phase extraction disks and subsequently analyzed by gas chromatography-mass spectrometry, finding the method suitable for the determination of individual herbicides in drinking water at concentrations of approximately 10 ng/L.
13 citations
TL;DR: The exposure of the two children was possibly due to a combination of pathways including exposure at the workshop itself during visits and also the transportation of lead-containing dust to the home environment.
3 citations
2 citations