Showing papers by "Sichuan University published in 2010"
Chinese Academy of Sciences1, Shanghai Jiao Tong University2, Fudan University3, Kunming Institute of Zoology4, Shenzhen University5, Chengdu Research Base of Giant Panda Breeding6, Wellcome Trust7, University of Toronto8, University of California, Berkeley9, Southeast University10, University of Hong Kong11, Sun Yat-sen University12, University of Vienna13, Cardiff University14, Comenius University in Bratislava15, Sichuan University16, South China University of Technology17, University of Copenhagen18, University of Alberta19, University of Washington20
TL;DR: Using next-generation sequencing technology alone, a draft sequence of the giant panda genome is generated and assembled, indicating that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition.
Abstract: Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes.
1,109 citations
TL;DR: The STandards for Reporting Interventions in Clinical Trials of Acupuncture (STRICTA) as mentioned in this paper were published in five journals in 2001 and 2002 and were designed to improve reporting of acupuncture trials, particularly the interventions, thereby facilitating their interpretation and replication.
Abstract: The STandards for Reporting Interventions in Clinical Trials of Acupuncture (STRICTA) were published in five journals in 2001 and 2002. These guidelines, in the form of a checklist and explanations for use by authors and journal editors, were designed to improve reporting of acupuncture trials, particularly the interventions, thereby facilitating their interpretation and replication. Subsequent reviews of the application and impact of STRICTA have highlighted the value of STRICTA as well as scope for improvements and revision. To manage the revision process a collaboration between the STRICTA Group, the CONSORT Group, and the Chinese Cochrane Centre was developed in 2008. An expert panel with 47 participants was convened that provided electronic feedback on a revised draft of the checklist. At a subsequent face-to-face meeting in Freiburg, a group of 21 participants further revised the STRICTA checklist and planned dissemination. The new STRICTA checklist, which is an official extension of CONSORT, includes six items and 17 sub-items. These set out reporting guidelines for the acupuncture rationale, the details of needling, the treatment regimen, other components of treatment, the practitioner background, and the control or comparator interventions. In addition, and as part of this revision process, the explanations for each item have been elaborated, and examples of good reporting for each item are provided. In addition, the word “controlled” in STRICTA is replaced by “clinical,” to indicate that STRICTA is applicable to a broad range of clinical evaluation designs, including uncontrolled outcome studies and case reports. It is intended that the revised STRICTA, in conjunction with both the main CONSORT Statement and extension for nonpharmacologic treatment, will raise the quality of reporting of clinical trials of acupuncture.
897 citations
TL;DR: An understanding of the complementary oxidation interaction provides a basis for explaining quality deterioration in meat and also for developing strategies to maintain optimal sensory qualities.
787 citations
TL;DR: P predictive models for classifying a sample with respect to one of 13 endpoints indicative of lung or liver toxicity in rodents, or of breast cancer, multiple myeloma or neuroblastoma in humans are generated.
Abstract: Gene expression data from microarrays are being applied to predict preclinical and clinical endpoints, but the reliability of these predictions has not been established. In the MAQC-II project, 36 independent teams analyzed six microarray data sets to generate predictive models for classifying a sample with respect to one of 13 endpoints indicative of lung or liver toxicity in rodents, or of breast cancer, multiple myeloma or neuroblastoma in humans. In total, >30,000 models were built using many combinations of analytical methods. The teams generated predictive models without knowing the biological meaning of some of the endpoints and, to mimic clinical reality, tested the models on data that had not been used for training. We found that model performance depended largely on the endpoint and team proficiency and that different approaches generated models of similar performance. The conclusions and recommendations from MAQC-II should be useful for regulatory agencies, study committees and independent investigators that evaluate methods for global gene expression analysis.
753 citations
11 Mar 2010-Nuclear Instruments & Methods in Physics Research Section A-accelerators Spectrometers Detectors and Associated Equipment
TL;DR: In this article, the design and construction of BESIII, which is designed to study physics in the τ-charm energy region utilizing the new high luminosity BEPCII double ring e + e − collider, is discussed.
Abstract: This paper will discuss the design and construction of BESIII, which is designed to study physics in the τ -charm energy region utilizing the new high luminosity BEPCII double ring e + e − collider. The expected performance will be given based on Monte Carlo simulations and results of cosmic ray and beam tests. In BESIII, tracking and momentum measurements for charged particles are made by a cylindrical multilayer drift chamber in a 1 T superconducting solenoid. Charged particles are identified with a time-of-flight system based on plastic scintillators in conjunction with dE/dx (energy loss per unit pathlength) measurements in the drift chamber. Energies of electromagnetic showers are measured by a CsI(Tl) crystal calorimeter located inside the solenoid magnet. Muons are identified by arrays of resistive plate chambers in a steel magnetic yoke for the flux return. The level 1 trigger system, data acquisition system and the detector control system based on networked computers will also be described.
733 citations
TL;DR: A Statistical Dispersal-Vicariance Analysis (S-DIVA) is used to statistically evaluate the alternative ancestral ranges at each node in a tree accounting for phylogenetic uncertainty and uncertainty in DIVA optimization.
661 citations
TL;DR: Polydopamine-coating can be prepared in acidic, neutral and alkaline aqueous media by oxidant-induced polymerization, which is material-independent and multifunctional for surface modification as mentioned in this paper.
648 citations
TL;DR: The challenges of pharmacophore modeling and applications in drug discovery are discussed and recent advances and latest developments are described, which provide useful clues to the further development and application of pharmacophile approaches.
619 citations
Johns Hopkins University1, University of Iowa2, University of Pittsburgh3, Utah State University4, Peking University5, Johns Hopkins University School of Medicine6, National Institutes of Health7, University of Bergen8, Icahn School of Medicine at Mount Sinai9, Chang Gung University10, Wuhan University11, Peking Union Medical College12, Boston Children's Hospital13, National University of Singapore14, Yonsei University15, Sichuan University16, University of Southern Denmark17, Statens Serum Institut18, Oswaldo Cruz Foundation19, University of British Columbia20, Trinity College, Dublin21, University of Miami22
TL;DR: In this paper, case-parent trios were used in a genome-wide association study of cleft lip with and without cleft palate, and two previously identified regions (at chromosome 8q24 and IRF6) attained genomewide significance.
Abstract: Case-parent trios were used in a genome-wide association study of cleft lip with and without cleft palate. SNPs near two genes not previously associated with cleft lip with and without cleft palate (MAFB, most significant SNP rs13041247, with odds ratio (OR) per minor allele = 0.704, 95% CI 0.635-0.778, P = 1.44 x 10(-11); and ABCA4, most significant SNP rs560426, with OR = 1.432, 95% CI 1.292-1.587, P = 5.01 x 10(-12)) and two previously identified regions (at chromosome 8q24 and IRF6) attained genome-wide significance. Stratifying trios into European and Asian ancestry groups revealed differences in statistical significance, although estimated effect sizes remained similar. Replication studies from several populations showed confirming evidence, with families of European ancestry giving stronger evidence for markers in 8q24, whereas Asian families showed stronger evidence for association with MAFB and ABCA4. Expression studies support a role for MAFB in palatal development.
512 citations
TL;DR: The chemistry, biology, and procedures for safe and efficient irrigation are summarized and cutting-edge information on the most recent developments are provided.
495 citations
TL;DR: The basic principles and typical applications of this technique based on grating projected and fringe analysis, which attracts the attention and research effort in the past ten years, has been targeted as main objective to review.
TL;DR: It is illustrated that miRNAs are important regulators of oligodendrocyte differentiation, providing new targets for myelin repair by selectively deleting a miRNA-processing enzyme in oligodendedrocytes of spinal cord mice lacking Dicer1.
TL;DR: In this paper, a discrepancy in the characteristic FTIR spectrum of the secondary layer in bilayer oleic acid-coated Fe3O4 nanoparticles was resolved through using FTIR and TGA together with dispersibility.
TL;DR: The M1macrophage density in the tumor islets is an independent predictor of survival time in NSCLC patients and the M2 macrophage densities were not associated with patient's survival time.
Abstract: Tumor-associated macrophages (TAMs) play an important role in growth, progression and metastasis of tumors. In non-small cell lung cancer (NSCLC), TAMs' anti-tumor or pro-tumor role is not determined. Macrophages are polarized into M1 (with anti-tumor function) and M2 (with pro-tumor function) forms. This study was conducted to determine whether the M1 and M2 macrophage densities in NSCLC are associated with patient's survival time. Fifty patients with an average of 1-year survival (short survival group) and 50 patients with an average of 5-year survival (long survival group) were included in this retrospective study. Paraffin-embedded NSCLC specimens and their clinicopathological data including up to 8-year follow-up information were used. Immunohistochemical double-staining of CD68/HLA-DR (markers for M1 macrophages) and CD68/CD163 (markers for M2 macrophages) was performed and evaluated in a blinded fashion. The M1 and M2 macrophage densities in the tumor islets, stroma, or islets and stroma were determined using computer-aided microscopy. Correlation of the macrophage densities and patient's survival time was analyzed using the Statistical Package for the Social Sciences. Approximately 70% of TAMs were M2 macrophages and the remaining 30% were M1 macrophages in NSCLC. The M2 macrophage densities (approximately 78 to 113 per mm2) in the tumor islets, stroma, or islets and stroma were not significantly different between the long survival and short survival groups. The M1 macrophage densities in the tumor islets (approximately 70/mm2) and stroma (approximately 34/mm2) of the long survival group were significantly higher than the M1 macrophage densities in the tumor islets (approximately 7/mm2) and stroma (13/mm2) of the short survival group (P < 0.001 and P < 0.05, respectively). The M2 macrophage densities were not associated with patient's survival time. The M1 macrophage densities in the tumor islets, stroma, or islets and stroma were positively associated with patient's survival time in a univariate analysis (P < 0.01 or 0.001). In a multivariate Cox proportional hazards analysis, the M1 macrophage density in the tumor islets was an independent predictor of patient's survival time. The M1 macrophage density in the tumor islets is an independent predictor of survival time in NSCLC patients.
TL;DR: Experiments show that, for rich-grained digital image, the capability of nonlinearly enhancing complex texture details in smooth area by fractional differential-based approach appears obvious better than by traditional integral-based algorithms.
Abstract: In this paper, we intend to implement a class of fractional differential masks with high-precision. Thanks to two commonly used definitions of fractional differential for what are known as Grumwald-Letnikov and Riemann-Liouville, we propose six fractional differential masks and present the structures and parameters of each mask respectively on the direction of negative x-coordinate, positive x-coordinate, negative y-coordinate, positive y-coordinate, left downward diagonal, left upward diagonal, right downward diagonal, and right upward diagonal. Moreover, by theoretical and experimental analyzing, we demonstrate the second is the best performance fractional differential mask of the proposed six ones. Finally, we discuss further the capability of multiscale fractional differential masks for texture enhancement. Experiments show that, for rich-grained digital image, the capability of nonlinearly enhancing complex texture details in smooth area by fractional differential-based approach appears obvious better than by traditional integral-based algorithms.
Memorial Sloan Kettering Cancer Center1, Cleveland Clinic2, Sichuan University3, Oregon Health & Science University4, Indiana University – Purdue University Indianapolis5, University of Hong Kong6, Katholieke Universiteit Leuven7, Medical University of South Carolina8, Fox Chase Cancer Center9, University of Texas MD Anderson Cancer Center10, University of Rochester11
TL;DR: Greater extent of lymphadenectomy was associated with increased survival for all patients with esophageal cancer except at the extremes (TisN0M0 and ≥7 regional lymph nodes positive for cancer) and well-differentiated pN 0M0 cancer.
Abstract: Objective:Using Worldwide Esophageal Cancer Collaboration data, we sought to (1) characterize the relationship between survival and extent of lymphadenectomy, and (2) from this, define optimum lymphadenectomy.Summary Background Data:What constitutes optimum lymphadenectomy to maximize survival is co
TL;DR: An efficient methodology for the synthesis of unsymmetrical biheteroaryl molecules has been developed via Pd(II)-catalyzed oxidative C-H/C-H cross-coupling of heteroarenes via an inversion in reactivity and selectivity.
Abstract: An efficient methodology for the synthesis of unsymmetrical biheteroaryl molecules has been developed via Pd(II)-catalyzed oxidative C−H/C−H cross-coupling of heteroarenes. An inversion in reactivity and selectivity has been achieved successfully to perform the desired heterocoupling. This process allows the heterocoupling of not only electron-rich N-containing heteroarenes (e.g., xanthines, azoles, and indolizines) but also electron-poor pyridine N-oxides with various thiophenes or furans.
Zhengzhou University1, Anhui Medical University2, Xinjiang Medical University3, Shihezi University4, Henan University5, Capital Medical University6, Henan University of Science and Technology7, Shandong University8, Inner Mongolia Medical University9, Shantou University10, Sichuan University11, Nanjing Medical University12, Fujian Medical University13, Kunming Institute of Zoology14, Beijing Jishuitan Hospital15, Zhejiang University16, University of Illinois at Chicago17, University of Medicine and Dentistry of New Jersey18, Peking University19
TL;DR: A previously unknown susceptibility locus for ESCC is identified: PLCE1 at 10q23, which might regulate cell growth, differentiation, apoptosis and angiogenesis and has important biological implications for both ESCC and GCA.
Abstract: Li Dong Wang and colleagues report a genome wide association study for esophageal squamous cell carcinoma in the Chinese population. They identify two risk loci at PLCE1 and C20orf54.
deCODE genetics1, Flinders Medical Centre2, University of Melbourne3, University of Iceland4, Yale University5, University of Akureyri6, The Chinese University of Hong Kong7, Guy's and St Thomas' NHS Foundation Trust8, Flinders University9, Cleveland Clinic10, University of Sydney11, Royal Cornhill Hospital12, Sichuan University13, King's College London14, QIMR Berghofer Medical Research Institute15, University of Southampton16, Duke University17, University Hospitals of Leicester NHS Trust18, Shantou University19, Uppsala University20, University of Western Australia21, University of Tasmania22
TL;DR: The risk variant identified here is located close to CAV1 and CAV2, both of which are expressed in the trabecular meshwork and retinal ganglion cells that are involved in the pathogenesis of POAG.
Abstract: We conducted a genome-wide association study for primary open-angle glaucoma (POAG) in 1,263 affected individuals (cases) and 34,877 controls from Iceland. We identified a common sequence variant at 7q31 (rs4236601[ A], odds ratio (OR) = 1.36, P = 5.0 x 10(-10)). We then replicated the association in sample sets of 2,175 POAG cases and 2,064 controls from Sweden, the UK and Australia (combined OR = 1.18, P = 0.0015) and in 299 POAG cases and 580 unaffected controls from Hong Kong and Shantou, China (combined OR = 5.42, P = 0.0021). The risk variant identified here is located close to CAV1 and CAV2, both of which are expressed in the trabecular meshwork and retinal ganglion cells that are involved in the pathogenesis of POAG.
TL;DR: Mucosal inflammation in nasal polyps orchestrated by T(H)2 cytokines and amplified by S aureus enterotoxins is characterized by an increased eosinophilic inflammation and formation of IgE antibodies.
Abstract: Background Nasal polyps often are associated with asthma. The phenotype of these patients is unknown. Objective To identify the mucosal factors associated with asthma comorbidity, we analyzed the inflammatory patterns of nasal polyps. Methods Nasal polyps from 70 Belgian patients, 34% with asthma, were analyzed for type of inflammation, T-cell cytokines, and IgE antibodies to Staphylococcus aureus enterotoxins. The same investigations were repeated in 93 Chinese patients with polyps, a group with a low asthma comorbidity rate (8%). Results In Belgian patients with polyps, 54% of samples showed eosinophilic inflammation. A classification tree evaluation identified IL-5 as the main positive determinant. Enterotoxin IgE in tissue (37%) was associated with significantly increased total IgE and eosinophil cationic protein concentrations. Expression of enterotoxin IgE, total IgE at greater than 1,442 kU/L, and eosinophil cationic protein at greater than 17,109 μg/L in samples with a total IgE concentration of greater than 246 kU/L significantly predicted asthma (odds ratio, 5.8-13). Only 7.5% of the samples from Chinese patients with polyps showed eosinophilic inflammation. IL-5 was confirmed as a positive determinant of eosinophilic inflammation, and enterotoxin IgE in tissue (17% of patients) was associated with significantly increased total IgE and eosinophil cationic protein concentrations. The expression of IL-5 or total IgE at greater than 790 kU/L in samples with an IL-5 concentration of greater than 194 pg/mL significantly predicted comorbid asthma (odds ratio, 17.2-96). Conclusion Mucosal inflammation in nasal polyps orchestrated by T H 2 cytokines and amplified by S aureus enterotoxins is characterized by an increased eosinophilic inflammation and formation of IgE antibodies. This phenotype is associated with comorbid asthma in white and Asian patients with nasal polyps.
TL;DR: In this paper, the effect of internal concentration polarization (ICP) on the flux of a spiral wound forward osmosis module (SWFO) was investigated under both submerged and cross-flow conditions.
TL;DR: In old age, the associations of depression with some chronic diseases were definite, but it should be further investigated whether arthritis, hypertension and diabetes were risk factors for increased depression or not.
TL;DR: It is demonstrated for the first time, to the knowledge, that widespread increased regional synchronous neural activity occurs after antipsychotic therapy, accompanied by decreased integration of function across widely distributed neural networks.
Abstract: Context: Most of what we know about antipsychotic drug effects is at the receptor level, distal from the neural system effects that mediate their clinical efficacy. Studying cerebral function in antipsychotic-naive patients with schizophrenia before and after pharmacotherapy can enhance understanding of the therapeutic mechanisms of these clinically effective treatments.
TL;DR: The results indicated that the bio-oils from catalytic pyrolysis of Nannochloropsis sp. residue (BOCP) had lower oxygen content and higher heating-value than those obtained from direct pyrolynsis (BODP) which had an oxygen content of 30.1 wt.% and heating- value of 24.6 MJ kg(-1).
TL;DR: In this paper, the effects of differently dimensional nanoparticles on the crystallization behavior of poly(l-lactide) matrices were investigated using time-resolved Fourier transform infrared spectroscopy (FTIR) and wide-angle X-ray diffraction (WAXD).
Abstract: Low-dimensional nanoparticles have a strong ability to induce the crystallization of polymer matrices. One-dimensional carbon nanotubes (CNTs) and two-dimensional graphene nanosheets (GNSs), both of which are both carbon-based nanoparticles, provide a good opportunity to investigate the effects of differently dimensional nanoparticles on the crystallization behavior of a polymer. For this purpose, respective nanocomposites of CNTs and GNSs with poly(l-lactide) (PLLA) as matrix were prepared by solution coagulation. Time-resolved Fourier-transform infrared spectroscopy (FTIR) and synchrotron wide-angle X-ray diffraction (WAXD) were performed to probe chain conformational changes and to determine the crystallization kinetics during the isothermal crystallization of the PLLA nanocomposites and neat PLLA, especially in the early stages. Both CNTs and GNSs could serve as nucleating agents in accelerating the crystallization kinetics of PLLA; however, the ability of CNTs to induce crystallization was stronger t...
TL;DR: A marked underrecruitment of the amygdala, accompanied by a substantial limitation in activation throughout a ventral temporal-basal ganglia-prefrontal cortex "social brain" system may be central to the difficulties patients experience when processing facial emotion.
Abstract: Background: People with schizophrenia have difficulty with emotion perception. Functional imaging studies indicate regional brain activation abnormalities in patients with schizophrenia when processing facial emotion. However, findings have not been entirely consistent across different studies. Methods: Activation likelihood estimation (ALE) meta-analyses were conducted to examine brain activation during facial emotion processing in patients with schizophrenia, controls, and patients compared with controls. Secondary meta-analyses were performed to assess the contribution of task design and illness chronicity to the results reported. Results: When processing facial expressions of emotions, both patients with schizophrenia and healthy controls activated the bilateral amygdala and right fusiform gyri. However, the extent of activation in these regions was generally much more limited in the schizophrenia samples. When directly compared with controls, the extent of activation in bilateral amygdala, parahippocampal gyrus and fusiform gyrus, right superior frontal gyrus, and lentiform nucleus was significantly less in patients. Patients with schizophrenia, but not controls, activated the left insula. A relative failure to recruit the amygdala in patients occurred regardless of whether the task design was explicit or implicit, while differences in fusiform activation were evident in explicit, not implicit, tasks. Restricting the analysis to patients with chronic illness did not substantially change the results. Conclusions: A marked underrecruitment of the amygdala, accompanied by a substantial limitation in activation throughout a ventral temporal-basal ganglia-prefrontal cortex "social brain" system may be central to the difficulties patients experience when processing facial emotion.
TL;DR: In this article, a graphene nanosheet/ultra-high molecular weight polyethylene composite with a segregated structure has been fabricated using water/ethanol solvent-assisted dispersion and hot compression at 200 °C.
TL;DR: A relationship between functional connectivity and disease severity was found in specific regions of RSNs, including medial and lateral prefrontal cortex, as well as parietal and occipital regions.
TL;DR: A self-nanoemulsifying drug delivery system (SNEDDS) for the oral delivery of Zedoary turmeric oil (ZTO), an essential oil extracted from the dry rhizome of Curcuma zedoaria, stored at 25 degrees C for at least 12 months is developed.
TL;DR: It is demonstrated that 10% SrHA coatings could enhance implant osseointegration in OVX rats, and the feasibility of using SrHA Coatings to improve implant fixation in osteoporotic bone is suggested.