Southeast University

About: Southeast University is a education organization based out in Nanjing, China. It is known for research contribution in the topics: MIMO & Control theory. The organization has 66363 authors who have published 79434 publications receiving 1170576 citations. The organization is also known as: SEU.

LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes.

Abstract: Pancreatic cancer is one of the most lethal malignancies and has an extremely poor diagnosis and prognosis. The development of resistance to gemcitabine is still a major challenge. The long noncoding RNA PVT1 was reported to be involved in carcinogenesis and chemoresistance; however, the mechanism by which PVT1 regulates the sensitivity of pancreatic cancer to gemcitabine remains poorly understood. The viability of pancreatic cancer cells was assessed by MTT assay in vitro and xenograft tumor formation assay in vivo. The expression levels of PVT1 and miR-619-5p were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Western blotting analysis and qRT-PCR were performed to assess the protein and mRNA levels of Pygo2 and ATG14, respectively. Autophagy was explored via autophagic flux detection under confocal microscopy and autophagic vacuole investigation under transmission electron microscopy (TEM). The functional role and mechanism of PVT1 were further investigated by gain- and loss-of-function assays in vitro. In the present study, we demonstrated that PVT1 was up-regulated in gemcitabine-resistant pancreatic cancer cell lines. Gain- and loss-of-function assays revealed that PVT1 impaired sensitivity to gemcitabine in vitro and in vivo. We further found that PVT1 up-regulated the expression of both Pygo2 and ATG14 and thus regulated Wnt/β-catenin signaling and autophagic activity to overcome gemcitabine resistance through sponging miR-619-5p. Moreover, we discovered three TCF/LEF binding elements (TBEs) in the promoter region of PVT1, and activation of Wnt/β-catenin signaling mediated by the up-regulation of Pygo2 increased PVT1 expression by direct binding to the TBE region. Furthermore, PVT1 was discovered to interact with ATG14, thus promoting assembly of the autophagy specific complex I (PtdIns3K-C1) and ATG14-dependent class III PtdIns3K activity. These data indicate that PVT1 plays a critical role in the sensitivity of pancreatic cancer to gemcitabine and highlight its potential as a valuable target for pancreatic cancer therapy.

Optimal Planning for Electricity-Hydrogen Integrated Energy System Considering Power to Hydrogen and Heat and Seasonal Storage

Abstract: For the future development of an integrated energy system (IES) with ultra-high penetration of renewable energy, a planning model for an electricity-hydrogen integrated energy system (EH-IES) is proposed with the considerations of hydrogen production and storage technologies. In this EH-IES, a reasonable power to heat and hydrogen (P2HH) model with startup/shutdown constraints and a novel model of seasonal hydrogen storage (SHS) are proposed for the first time. To cope with the challenges faced by EH-IES, we use a combination of stochastic and robust optimization approaches to address the generation-load uncertainties, making the planning results robust. The N-1 contingency of crucial devices is further considered to make the system reliable. Additionally, the effects of the annual ratios of different seasons on the optimal allocation and operation of SHS are investigated. The min-max-min model with binary variables is solved by a nested column-and-constraint generation algorithm. Finally, case studies verified the advantages and effectiveness of the proposed model and planning method.

Soft and hard templating of graphitic carbon nitride

Abstract: Graphitic carbon nitride (g-CN) is an exciting material – a semiconductor comprised only of carbon and nitrogen. It is very easy to synthesize and there are many papers on the development of this material in key energy applications such as photoelectrochemical (PEC) conversion of solar energy into chemical fuels. As a promising candidate for sustainable photocathodes, g-CN has advantages such as low cost, visible light sensitivity and high chemical stability. However, to date, the performance of g-CN has been limited, partly because standard synthesis methods produce relatively dense materials with low surface area. To combat this, there are now many examples of hard and soft templating to change the structure and morphology of g-CN and introduce porosity. This review will discuss the key advances and challenges in this interesting new field.