scispace - formally typeset
Search or ask a question
Institution

Southern Illinois University School of Medicine

EducationSpringfield, Illinois, United States
About: Southern Illinois University School of Medicine is a education organization based out in Springfield, Illinois, United States. It is known for research contribution in the topics: Population & Cancer. The organization has 3747 authors who have published 5977 publications receiving 209115 citations. The organization is also known as: SIU School of Medicine.
Topics: Population, Cancer, Ototoxicity, Epilepsy, Receptor


Papers
More filters
Journal ArticleDOI
TL;DR: Obese subjects had significantly lower basal 25-hydroxyvitamin D concentrations and higher parathyroid hormone concentrations than did age-matched control subjects and the incremental increase in vitamin D(3) was 57% lower in obese than in nonobese subjects.

2,980 citations

Journal ArticleDOI
TL;DR: High survival was achieved with osmolarity lower than found in Dulbecco's Modified Eagle's Medium (DMEM), and by reducing cysteine and glutamine concentrations and by the elimination of toxic ferrnous sulphate found in DME/F12, and Neurobasal is a new medium that incorporates modifications to DMEM.
Abstract: We have systematically optimized the concentrations of 20 components of a previously published serum-free medium (Brewer and Cotman, Brain Res 494: 65-74, 1989) for survival of rat embryonic hippocampal neurons after 4 days in culture. This serum-free medium supplement, B27, produced neuron survival above 60%, independent of plating density above 160 plated cells/mm2. For isolated cells (< 100 cells/mm2), survival at 4 days was still above 45%, but could be rescued to the 60% level at 40 cells/mm2 by simply applying a coverslip on top of the cells. This suggests a need for additional trophic factors. High survival was achieved with osmolarity lower than found in Dulbecco's Modified Eagle's Medium (DMEM), and by reducing cysteine and glutamine concentrations and by the elimination of toxic ferrous sulphate found in DME/F12. Neurobasal is a new medium that incorporates these modifications to DMEM. In B27/Neurobasal, glial growth is reduced to less than 0.5% of the nearly pure neuronal population, as judged by immunocytochemistry for glial fibrillary acidic protein and neuron-specific enolase. Excellent long-term viability is achieved after 4 weeks in culture with greater than 90% viability for cells plated at 640/mm2 and greater than 50% viability for cells plated at 160/mm2. Since the medium also supports the growth of neurons from embryonic rat striatum, substantia nigra, septum, and cortex, and neonatal dentate gyrus and cerebellum (Brewer, in preparation), support for other neuron types is likely. B27/Neurobasal should be useful for in vitro studies of neuronal toxicology, pharmacology, electrophysiology, gene expression, development, and effects of growth factors and hormones.

2,222 citations

Journal ArticleDOI
TL;DR: A taxonomy is proposed to facilitate an awareness of differences in quality and in the educational objectives that can be achieved and to help teachers choose a problem‐based learning method most appropriate for their students.
Abstract: The increasingly popular term 'problem-based learning' does not refer to a specific educational method It can have many different meanings depending on the design of the educational method employed and the skills of the teacher The many variables possible can produce wide variations in quality and in the educational objectives that can be achieved A taxonomy is proposed to facilitate an awareness of these differences and to help teachers choose a problem-based learning method most appropriate for their students

1,933 citations

Journal ArticleDOI
26 Apr 2007-Oncogene
TL;DR: The results suggest that miR-21 functions as an oncogene and modulates tumorigenesis through regulation of genes such as bcl-2 and thus, it may serve as a novel therapeutic target.
Abstract: MicroRNAs (miRNAs) are ∼22 nucleotide non-coding RNA molecules that regulate gene expression post-transcriptionally. Although aberrant expression of miRNAs in various human cancers suggests a role for miRNAs in tumorigenesis, it remains largely unclear as to whether knockdown of a specific miRNA affects tumor growth. In this study, we profiled miRNA expression in matched normal breast tissue and breast tumor tissues by TaqMan real-time polymerase chain reaction miRNA array methods. Consistent with previous findings, we found that miR-21 was highly overexpressed in breast tumors compared to the matched normal breast tissues among 157 human miRNAs analysed. To better evaluate the role of miR-21 in tumorigenesis, we transfected breast cancer MCF-7 cells with anti-miR-21 oligonucleotides and found that anti-miR-21 suppressed both cell growth in vitro and tumor growth in the xenograft mouse model. Furthermore, this anti-miR-21-mediated cell growth inhibition was associated with increased apoptosis and decreased cell proliferation, which could be in part owing to downregulation of the antiapoptotic Bcl-2 in anti-miR-21-treated tumor cells. Together, these results suggest that miR-21 functions as an oncogene and modulates tumorigenesis through regulation of genes such as bcl-2 and thus, it may serve as a novel therapeutic target.

1,541 citations

Journal ArticleDOI
TL;DR: The authors reviewed the motivation for the change to problem-based learning, its definition, and the educational objectives it can serve, and discussed changing an established curriculum to Problem-Based Learning and asked whether problembased learning is worth the trouble.
Abstract: This chapter reviews the motivation for the change to problem-based learning, its definition, and the educational objectives it can serve. It discusses changing an established curriculum to problem-based learning and asks whether problem-based learning is worth the trouble.

1,504 citations


Authors

Showing all 3778 results

NameH-indexPapersCitations
Jatin P. Shah11972545680
Harold G. Koenig9967846742
Chawnshang Chang9753435629
Richard J. K. Taylor91154343893
Martin R. Farlow8238126820
David A. D'Alessio8027222955
Dirk R. Larson7927124067
Andrzej Bartke7851622865
Michael Brenner7656422010
Arnulf Stenzl7379123285
Wolfgang H. Dillmann7220017595
Michael Bonkowski6627913851
Jacob E. Friedman6519112485
Richard Salvi6544716289
Russell Noyes6322912790
Network Information
Related Institutions (5)
Icahn School of Medicine at Mount Sinai
76K papers, 3.7M citations

95% related

Johns Hopkins University School of Medicine
79.2K papers, 4.7M citations

94% related

Baylor College of Medicine
94.8K papers, 5M citations

94% related

University of Texas Southwestern Medical Center
75.2K papers, 4.4M citations

94% related

Oregon Health & Science University
65.1K papers, 3.3M citations

94% related

Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20237
202233
2021281
2020276
2019221
2018177