Southwest University

About: Southwest University is a education organization based out in Chongqing, China. It is known for research contribution in the topics: Gene & Population. The organization has 29772 authors who have published 27755 publications receiving 409441 citations. The organization is also known as: Southwest University in Chongqing & SWU.

GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.).

Abstract: The bZIP transcription factor (TF) act as an important regulator for the abscisic acid (ABA) mediated abiotic stresses signaling pathways in plants. Here, we reported the cloning and characterization of GhABF2, encoding for typical cotton bZIP TF. Overexpression of GhABF2 significantly improved drought and salt stress tolerance both in Arabidopsis and cotton. However, silencing of GhABF2 made transgenic cotton sensitive to PEG osmotic and salt stress. Expression of GhABF2 was induced by drought and ABA treatments but repressed by high salinity. Transcriptome analysis indicated that GhABF2 increases drought and salt tolerance by regulating genes related to ABA, drought and salt response. The proline contents, activity of superoxide dismutase (SOD) and catalase (CAT) were also significantly increased in GhABF2-overexpression cottons in comparison to wild type after drought and salt treatment. Further, an increase in fiber yield under drought and saline-alkali wetland exhibited the important role of GhABF2 in enhancing the drought and salt tolerance in transgenic lines. In conclusion, manipulation of GhABF2 by biotechnological tools could be a sustainable strategy to deploy drought and salt tolerance in cotton.

An ECL sensor for dopamine using reduced graphene oxide/multiwall carbon nanotubes/gold nanoparticles

Abstract: An eletrochemiluminescence (ECL) sensor based on ECL of peroxydisulfate solution for detecting the dopamine (DA) was proposed using reduced graphene oxide/multiwall carbon nanotubes/gold nanoparticles (rGO/MWCNTs/AuNPs) hybrid modified glassy carbon electrode (GCE). The rGO/MWCNTs/AuNPs was synthesized by an effective and facile in situ chemical reaction using polyethyleneimine (PEI) as a reducing agent for both GO/MWCNTs and AuCl 4 − . The ECL behaviors of peroxydisulfate solution have been investigated at the rGO/MWCNTs/AuNPs/GCE, and DA was found to be able to enhance the ECL of peroxydisulfate solution. Under the optimized conditions, the enhanced ECL signal intensity of peroxydisulfate solution was linear with the concentration of DA in the range between 0.20 and 70 μM ( R = 0.9902) with a detection limit ( S / N = 3) of 0.067 μM. Furthermore, the developed sensor exhibited a high sensitivity, good reproducibility, stability for the detection of DA. The applicability of the proposed sensor was also evaluated by detecting DA in dopamine hydrochloride injection, human urine and serum.