scispace - formally typeset
Search or ask a question
Institution

Spanish National Research Council

GovernmentMadrid, Spain
About: Spanish National Research Council is a government organization based out in Madrid, Spain. It is known for research contribution in the topics: Population & Galaxy. The organization has 79563 authors who have published 220470 publications receiving 7698991 citations. The organization is also known as: CSIC & Consejo Superior de Investigaciones Científicas.
Topics: Population, Galaxy, Catalysis, Stars, Star formation


Papers
More filters
Journal ArticleDOI
14 Oct 2004-Nature
TL;DR: The purification and functional characterization of an E3 ubiquitin ligase complex that is specific for histone H2A is reported, and it is linked to Polycomb silencing, which is important in regulating chromatin dynamics and transcription.
Abstract: Covalent modification of histones is important in regulating chromatin dynamics and transcription1,2. One example of such modification is ubiquitination, which mainly occurs on histones H2A and H2B3. Although recent studies have uncovered the enzymes involved in histone H2B ubiquitination4,5,6 and a ‘cross-talk’ between H2B ubiquitination and histone methylation7,8, the responsible enzymes and the functions of H2A ubiquitination are unknown. Here we report the purification and functional characterization of an E3 ubiquitin ligase complex that is specific for histone H2A. The complex, termed hPRC1L (human Polycomb repressive complex 1-like), is composed of several Polycomb-group proteins including Ring1, Ring2, Bmi1 and HPH2. hPRC1L monoubiquitinates nucleosomal histone H2A at lysine 119. Reducing the expression of Ring2 results in a dramatic decrease in the level of ubiquitinated H2A in HeLa cells. Chromatin immunoprecipitation analysis demonstrated colocalization of dRing with ubiquitinated H2A at the PRE and promoter regions of the Drosophila Ubx gene in wing imaginal discs. Removal of dRing in SL2 tissue culture cells by RNA interference resulted in loss of H2A ubiquitination concomitant with derepression of Ubx. Thus, our studies identify the H2A ubiquitin ligase, and link H2A ubiquitination to Polycomb silencing.

1,685 citations

Journal ArticleDOI
TL;DR: This review describes the principles of formation and stability of nano-emulsions, namely high energy emulsification (using homogenisers), low energy emulsion preparation, and the principle of the phase inversion temperature (PIT), and the role of the adsorbed layer thickness.

1,662 citations

Journal ArticleDOI
TL;DR: The Snail superfamily of zinc-finger transcription factors is involved in processes that imply pronounced cell movements, both during embryonic development and in the acquisition of invasive and migratory properties during tumour progression.
Abstract: The Snail superfamily of zinc-finger transcription factors is involved in processes that imply pronounced cell movements, both during embryonic development and in the acquisition of invasive and migratory properties during tumour progression. Different family members have also been implicated in the signalling cascade that confers left–right identity, as well as in the formation of appendages, neural differentiation, cell division and cell survival.

1,660 citations

Journal ArticleDOI
TL;DR: An updated version of wannier90 is presented, wannIER90 2.0, including minor bug fixes and parallel (MPI) execution for band-structure interpolation and the calculation of properties such as density of states, Berry curvature and orbital magnetisation.

1,654 citations

Journal ArticleDOI
TL;DR: A novel fluorimetric method to estimate the G+C mol% content in microorganisms using double-stranded DNA and its thermal denaturalization was followed by measuring a decrease in fluorescence using a real-time PCR thermocycler.
Abstract: G+C mol% content in microorganisms is one of the recommended characteristics for the standard description of bacterial species. In this study we present a novel fluorimetric method to estimate the G+C mol% content in microorganisms. Double-stranded DNA was specifically stained with SYBR Green I, and its thermal denaturalization was followed by measuring a decrease in fluorescence using a real-time PCR thermocycler. Unlike most previous determinations of G+C mol%, in this study only DNA from microorganisms with an available completely sequenced genome were used to prepare the calibration curves. Calibration curves showed a linear relationship between G+C mol% content and melting temperature and they were performed both in the absence and presence of 30% formamide. This protocol proves to be a rapid and inexpensive method to estimate DNA base ratios of novel microorganisms.

1,650 citations


Authors

Showing all 79686 results

NameH-indexPapersCitations
Guido Kroemer2361404246571
George Efstathiou187637156228
Peidong Yang183562144351
H. S. Chen1792401178529
David R. Williams1782034138789
Andrea Bocci1722402176461
Adrian L. Harris1701084120365
Gang Chen1673372149819
Gregory J. Hannon165421140456
Alvaro Pascual-Leone16596998251
Jorge E. Cortes1632784124154
Dongyuan Zhao160872106451
John B. Goodenough1511064113741
David D'Enterria1501592116210
A. Gomes1501862113951
Network Information
Related Institutions (5)
Centre national de la recherche scientifique
382.4K papers, 13.6M citations

95% related

University of Barcelona
108.5K papers, 3.7M citations

93% related

Max Planck Society
406.2K papers, 19.5M citations

93% related

Chinese Academy of Sciences
634.8K papers, 14.8M citations

93% related

University of Padua
114.8K papers, 3.6M citations

92% related

Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20241
202371
2022463
202111,933
202012,584
201911,596