Swedish University of Agricultural Sciences

About: Swedish University of Agricultural Sciences is a education organization based out in Uppsala, Sweden. It is known for research contribution in the topics: Population & Soil water. The organization has 13510 authors who have published 35241 publications receiving 1414458 citations. The organization is also known as: Sveriges Lantbruksuniversitet & SLU.

Insight into trade‐off between wood decay and parasitism from the genome of a fungal forest pathogen

Abstract: Summary • Parasitism and saprotrophic wood decay are two fungal strategies fundamental for succession and nutrient cycling in forest ecosystems. An opportunity to assess the trade-off between these strategies is provided by the forest pathogen and wood decayer Heterobasidion annosum sensu lato. • We report the annotated genome sequence and transcript profiling, as well as the quantitative trait loci mapping, of one member of the species complex: H. irregulare. Quantitative trait loci critical for pathogenicity, and rich in transposable elements, orphan and secreted genes, were identified. • A wide range of cellulose-degrading enzymes are expressed during wood decay. By contrast, pathogenic interaction between H. irregulare and pine engages fewer carbohydrate-active enzymes, but involves an increase in pectinolytic enzymes, transcription modules for oxidative stress and secondary metabolite production. • Our results show a trade-off in terms of constrained carbohydrate decomposition and membrane transport capacity during interaction with living hosts. Our findings establish that saprotrophic wood decay and necrotrophic parasitism involve two distinct, yet overlapping, processes.

Automated delineation of individual tree crowns in high spatial resolution aerial images by multiple-scale analysis

Abstract: This paper presents an automatic multiple-scale algorithm for delineation of individual tree crowns in high spatial resolution infrared colour aerial images. The tree crown contours were identified as zero-crossings, with con- vex grey-level curvature, which were computed on the in- tensity image for each image scale. A modified centre of curvature was estimated for every edge segment pixel. For each segment, these centre points formed a swarm which was modelled as a primal sketch using an ellipse extended with the mean circle of curvature. The model described the region of the derived tree crown based on the edge segment at the current scale. The sketch was rescaled with a signif- icance value and accumulated for a scale interval. In the accumulated sketch, a tree crown segment was grown, start- ing at local peaks, under the condition that it was inside the area of healthy vegetation in the aerial image and did not trespass into a neighbouring crown segment. The method was evaluated by comparison with manual delineation and with ground truth on 43 randomly selected sample plots. It was concluded that the performance of the method is almost equivalent to visual interpretation. On the average, seven out of ten tree crowns were the same. Furthermore, ground truth indicated a large number of hidden trees. The proposed technique could be used as a basic tool in forest surveys.

Different Myrosinase and Idioblast Distribution in Arabidopsis and Brassica napus

Abstract: Myrosinase (EC 3.2.3.1) is a glucosinolate-degrading enzyme mainly found in special idioblasts, myrosin cells, in Brassicaceae. This two-component system of secondary products and degradative enzymes is important in plant-insect interactions. Immunocytochemical analysis of Arabidopsis localized myrosinase exclusively to myrosin cells in the phloem parenchyma, whereas no myrosin cells were detected in the ground tissue. In Brassica napus, myrosinase could be detected in myrosin cells both in the phloem parenchyma and in the ground tissue. The myrosin cells were similar in Arabidopsis and B. napus and were found to be different from the companion cells and the glucosinolate-containing S-cells present in Arabidopsis. Confocal laser scanning immunomicroscopy analysis of myrosin cells in B. napus embryos showed that the myrosin grains constitute a continuous reticular system in the cell. These findings indicate that in the two species studied, initial cells creating the ground tissue have different potential for making idioblasts and suggest that the myrosinase-glucosinolate system has at least partly different functions. Several myrosinases in B. napus extracts are recovered in complex together with myrosinase-binding protein (MBP), and the localization of MBP was therefore studied in situ. The expression of MBP was highest in germinating seedlings of B. napus and was found in every cell except the myrosin cells of the ground tissue. Rapid disappearance of the MBP from the non-myrosin cells and emergence of MBP in the myrosin cells resulted in an apparent colocalization of MBP and myrosinase in 7-d-old seedlings.

Promoting sustainable intensification in precision agriculture: review of decision support systems development and strategies

Abstract: Precision agriculture provides important issues toward a more sustainable agriculture. Many farmers have the necessary technology to operate site-specifically, but they do not use it in practice, a ...

Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis

Abstract: The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in plant development. Its spatiotemporal distribution is controlled by a combination of biosynthetic, metabolic and transport mechanisms. Four families of auxin transporters have been identified that mediate transport across the plasma or endoplasmic reticulum membrane. Here we report the discovery and the functional characterization of the first vacuolar auxin transporter. We demonstrate that WALLS ARE THIN1 (WAT1), a plant-specific protein that dictates secondary cell wall thickness of wood fibres, facilitates auxin export from isolated Arabidopsis vacuoles in yeast and in Xenopus oocytes. We unambiguously identify IAA and related metabolites in isolated Arabidopsis vacuoles, suggesting a key role for the vacuole in intracellular auxin homoeostasis. Moreover, local auxin application onto wat1 mutant stems restores fibre cell wall thickness. Our study provides new insight into the complexity of auxin transport in plants and a means to dissect auxin function during fibre differentiation.