Institution
Swedish University of Agricultural Sciences
Education•Uppsala, Sweden•
About: Swedish University of Agricultural Sciences is a education organization based out in Uppsala, Sweden. It is known for research contribution in the topics: Population & Soil water. The organization has 13510 authors who have published 35241 publications receiving 1414458 citations. The organization is also known as: Sveriges Lantbruksuniversitet & SLU.
Topics: Population, Soil water, Species richness, Biodiversity, Gene
Papers published on a yearly basis
Papers
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TL;DR: Results provide direct genetic evidence for the ABA-dependent regulation of the rab18 gene in A. thaliana.
Abstract: We have isolated a rab-related (responsive to ABA) gene, rab18 from Arabidopsis thaliana. The gene encodes a hydrophilic, glycine-rich protein (18.5 kDa), which contains the conserved serine- and lysine-rich domains characteristic of similar RAB proteins in other plant species. The rab18 mRNA accumulates in plants exposed to low temperature, water stress or exogenous ABA but not in plants subjected to heat shock. This stress-related accumulation of the rab18 mRNA is markedly decreased in the ABA-synthesis mutant aba-1, the ABA-response mutant abi-1 or in wild-type plants treated with the carotenoid synthesis inhibitor, fluridone. Exogenous ABA treatment can induce the rab18 mRNA in the aba-1 mutant but not in the abi-1 mutant. These results provide direct genetic evidence for the ABA-dependent regulation of the rab18 gene in A. thaliana.
429 citations
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TL;DR: The gas chromatographic-colorimetric-gravimetric method (Uppsala method) for determination of total dietary fiber (as neutral sugar residues, uronic acid residues, and Klason lignin) has been adopted first action by AOAC INTERNATIONAL.
Abstract: A joint AOAC/American Association of Cereal Chemists (AACC) collaborative study was conducted to determine by the Uppsala method the dietary fiber content and its composition in various foods. The method includes preparation of a residue by treatment with thermostable alpha-amylase and amyloglucosidase and then ethanol precipitation of solubilized dietary fiber components while leaving low-molecular weight carbohydrates in solution. After acid hydrolysis of residue, neutral polysaccharide residues are determined as alditol acetates by gas-liquid chromatography, uronic acid residues are determined by colorimetry, and ash-free acid-insoluble residue (Klason lignin) is determined gravimetrically. Total dietary fiber, including enzyme-resistant starch, is calculated as the sum of nonstarch polysaccharide residues and Klason lignin. Nine laboratories completed the study, analyzing in duplicate 8 unknown dried products that included 4 cereal products, green peas, potato fiber, carrots, and apples. Total dietary fiber contents of products tested ranged from 4.6 to 84.3%, with an average RSDR value of 8.4% (range, 4.8-11.1%). Total neutral polysaccharide residues ranged from 3.8 to 64.1%, with an average RSDR value of 7.5% (range, 5.4-10.5%). Individual neutral sugars (rhamnose, arabinose, xylose, mannose, galactose, and glucose) and uronic acid residues present at more than 1% generally had good RSDR values (3.3-22.8%), whereas, as expected for Klason lignin, only the wheat bran sample with a high content (16%) had an excellent RSDR value (5.0%). The gas chromatographic-colorimetric-gravimetric method (Uppsala method) for determination of total dietary fiber (as neutral sugar residues, uronic acid residues, and Klason lignin) has been adopted first action by AOAC INTERNATIONAL.
426 citations
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TL;DR: Using consensus regions in gene sequences encoding the two forms of nitrite reductase (Nir), a key enzyme in the denitrification pathway, two sets of PCR primers to amplifycd1- and Cu-nir were designed and conserved.
Abstract: Using consensus regions in gene sequences encoding the two forms of nitrite reductase (Nir), a key enzyme in the denitrification pathway, we designed two sets of PCR primers to amplify cd1- and Cu-nir. The primers were evaluated by screening defined denitrifying strains, denitrifying isolates from wastewater treatment plants, and extracts from activated sludge. Sequence relationships of nir genes were also established. The cd1 primers were designed to amplify a 778 to 799-bp region of cd1-nir in the six published sequences. Likewise, the Cu primers amplified a 473-bp region in seven of the eight published Cu-nir sequences. Together, the two sets of PCR primers amplified nir genes in nine species within four genera, as well as in four of the seven sludge isolates. The primers did not amplify genes of nondenitrifying strains. The Cu primers amplified the expected fragment in all 13 sludge samples, but cd1-nir fragments were only obtained in five samples. PCR products of the expected sizes were verified as nir genes after hybridization to DNA probes, except in one case. The sequenced nir fragments were related to other nir sequences, demonstrating that the primers amplified the correct gene. The selected primer sites for Cu-nir were conserved, while broad-range primers targeting conserved regions of cd1-nir seem to be difficult to find. We also report on the existence of Cu-nir in Paracoccus denitrificans Pd1222.
426 citations
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TL;DR: The results show that this approach allows a reliable protocol for metabolomic analysis of Arabidopsis to be determined with a relatively limited number of experiments, and it was shown that both hydrophilic and lipophilic compounds were detected with high reproducibility.
426 citations
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TL;DR: The observed time gap between tick origin and mammalian radiation indicates the existence of hitherto unknown piroplasmid lineages and/or species in extant vertebrate taxa, including reptiles and possibly amphibians.
425 citations
Authors
Showing all 13653 results
Name | H-index | Papers | Citations |
---|---|---|---|
Svante Pääbo | 147 | 407 | 84489 |
Lars Klareskog | 131 | 697 | 63281 |
Stephen Hillier | 129 | 1138 | 83831 |
Carol V. Robinson | 123 | 670 | 51896 |
Jun Yu | 121 | 1174 | 81186 |
Peter J. Anderson | 120 | 966 | 63635 |
David E. Clapham | 119 | 382 | 58360 |
Angela M. Gronenborn | 113 | 568 | 44800 |
David A. Wardle | 110 | 409 | 70547 |
Agneta Oskarsson | 106 | 766 | 40524 |
Jack S. Remington | 103 | 481 | 38006 |
Hans Ellegren | 102 | 349 | 39437 |
Per A. Peterson | 102 | 356 | 35788 |
Malcolm J. Bennett | 99 | 439 | 37207 |
Gunnar E. Carlsson | 98 | 466 | 32638 |