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Institution

Swedish University of Agricultural Sciences

EducationUppsala, Sweden
About: Swedish University of Agricultural Sciences is a education organization based out in Uppsala, Sweden. It is known for research contribution in the topics: Population & Soil water. The organization has 13510 authors who have published 35241 publications receiving 1414458 citations. The organization is also known as: Sveriges Lantbruksuniversitet & SLU.


Papers
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Journal ArticleDOI
TL;DR: A model in which monoubiquitin-dependent internalization/sorting and turnover keep the plasma membrane pool of IRT1 low to ensure proper iron uptake and to prevent metal toxicity is suggested.
Abstract: Plants take up iron from the soil using the IRON-REGULATED TRANSPORTER 1 (IRT1) high-affinity iron transporter at the root surface. Sophisticated regulatory mechanisms allow plants to tightly control the levels of IRT1, ensuring optimal absorption of essential but toxic iron. Here, we demonstrate that overexpression of Arabidopsis thaliana IRT1 leads to constitutive IRT1 protein accumulation, metal overload, and oxidative stress. IRT1 is unexpectedly found in trans-Golgi network/early endosomes of root hair cells, and its levels and localization are unaffected by iron nutrition. Using pharmacological approaches, we show that IRT1 cycles to the plasma membrane to perform iron and metal uptake at the cell surface and is sent to the vacuole for proper turnover. We also prove that IRT1 is monoubiquitinated on several cytosol-exposed residues in vivo and that mutation of two putative monoubiquitination target residues in IRT1 triggers stabilization at the plasma membrane and leads to extreme lethality. Together, these data suggest a model in which monoubiquitin-dependent internalization/sorting and turnover keep the plasma membrane pool of IRT1 low to ensure proper iron uptake and to prevent metal toxicity. More generally, our work demonstrates the existence of monoubiquitin-dependent trafficking to lytic vacuoles in plants and points to proteasome-independent turnover of plasma membrane proteins.

410 citations

Journal ArticleDOI
TL;DR: A new semiautomated strategy using a hierarchical multivariate curve resolution approach that processes all samples simultaneously is described that generates tables of all the detected metabolites that differ in relative concentrations between samples.
Abstract: In metabolomics, the objective is to identify differences in metabolite profiles between samples. A widely used tool in metabolomics investigations is gas chromatography−mass spectrometry (GC/MS). More than 400 compounds can be detected in a single analysis, if overlapping GC/MS peaks are deconvoluted. However, the deconvolution process is time-consuming and difficult to automate, and additional processing is needed in order to compare samples. Therefore, there is a need to improve and automate the data processing strategy for data generated in GC/MS-based metabolomics; if not, the processing step will be a major bottleneck for high-throughput analyses. Here we describe a new semiautomated strategy using a hierarchical multivariate curve resolution approach that processes all samples simultaneously. The presented strategy generates (after appropriate treatment, e.g., multivariate analysis) tables of all the detected metabolites that differ in relative concentrations between samples. The processing of 70 s...

409 citations

Journal ArticleDOI
TL;DR: This paper provides easy to use protocols for the collection, identification, diagnosis, rearing, breeding, marking and measurement of infestation rates and fertility of V. destructor and describes pros and cons for all methods for the user to know which method to use under which circumstances.
Abstract: SummaryVery rapidly after Varroa destructor invaded apiaries of Apis mellifera, the devastating effect of this mite prompted an active research effort to understand and control this parasite. Over a few decades, varroa has spread to most countries exploiting A. mellifera. As a consequence, a large number of teams have worked with this organism, developing a diversity of research methods. Often different approaches have been followed to achieve the same goal. The diversity of methods made the results difficult to compare, thus hindering our understanding of this parasite. In this paper, we provide easy to use protocols for the collection, identification, diagnosis, rearing, breeding, marking and measurement of infestation rates and fertility of V. destructor. We also describe experimental protocols to study orientation and feeding of the mite, to infest colonies or cells and measure the mite's susceptibility to acaricides. Where relevant, we describe which mite should be used for bioassays since their beha...

409 citations

Journal ArticleDOI
10 Jun 2011-Science
TL;DR: In this article, the effects of species gain and loss have been investigated. But the authors focus on traits associated with species loss, and much remains unknown about how traits that predispose species to extinction affect ecological processes.
Abstract: Ecosystems worldwide are losing some species and gaining others, resulting in an interchange of species that is having profound impacts on how these ecosystems function. However, research on the effects of species gains and losses has developed largely independently of one another. Recent conceptual advances regarding effects of species gain have arisen from studies that have unraveled the mechanistic basis of how invading species with novel traits alter biotic interactions and ecosystem processes. In contrast, studies on traits associated with species loss are fewer, and much remains unknown about how traits that predispose species to extinction affect ecological processes. Species gains and losses are both consequences and drivers of global change; thus, explicit integration of research on how both processes simultaneously affect ecosystem functioning is key to determining the response of the Earth system to current and future human activities.

408 citations

Journal ArticleDOI
TL;DR: In this article, an analysis of dissolved organic carbon in Swedish lakes found that aliphatic and N-containing compounds persisted, while oxidized aromatic compounds were lost, indicating that organic matter's molecular structure has been thought to influence its decomposition.
Abstract: Organic matter’s molecular structure has been thought to influence its decomposition. Analyses of dissolved organic carbon in Swedish lakes found that aliphatic and N-containing compounds persisted, while oxidized aromatic compounds were lost.

408 citations


Authors

Showing all 13653 results

NameH-indexPapersCitations
Svante Pääbo14740784489
Lars Klareskog13169763281
Stephen Hillier129113883831
Carol V. Robinson12367051896
Jun Yu121117481186
Peter J. Anderson12096663635
David E. Clapham11938258360
Angela M. Gronenborn11356844800
David A. Wardle11040970547
Agneta Oskarsson10676640524
Jack S. Remington10348138006
Hans Ellegren10234939437
Per A. Peterson10235635788
Malcolm J. Bennett9943937207
Gunnar E. Carlsson9846632638
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
2023116
2022252
20212,311
20201,957
20191,787
20181,624