Tabriz University of Medical Sciences

About: Tabriz University of Medical Sciences is a education organization based out in Tabriz, Iran. It is known for research contribution in the topics: Population & Cancer. The organization has 11499 authors who have published 17525 publications receiving 241099 citations.

Relationship between serum-soluble receptor for advanced glycation end products (sRAGE) and disease activity in rheumatoid arthritis patients.

Abstract: Objective: Considering the important role of serum soluble receptor for advanced glycation end product (sRAGE/RAGE)–ligand system in rheumatoid arthritis (RA), this study aimed to evaluate serum sR...

Mass-Production and Characterization of Anti-CD20 Monoclonal Antibody in Peritoneum of Balb/c Mice.

Abstract: Purpose: Monoclonal antibodies are important tools are used in basic research as well as, in diagnosis, imaging and treatment of immunodeficiency diseases, infections and cancers. The purpose of this study was to produce large scale of monoclonal antibody against CD20 in order to diagnostic application in leukemia and lymphomas disorders. Methods: Hybridoma cells that produce monoclonal antibody against human CD20 were administered into the peritoneum of the Balb/c mice which have previously been primed with 0.5 ml Pristane. After twelve days, approximately 7 ml ascetic fluid was harvested from the peritoneum of each mouse. Evaluation of mAb titration was assessed by ELISA method. In the present study, we describe a protocol for large scale production of MAbs. Results: We prepared monoclonal antibodies (mAbs) with high specificity and sensitivity against human CD20 by hybridoma method and characterized them by ELISA. The subclass of antibody was IgG2a and its light chain was kappa. Ascetic fluid was purified by Protein-A Sepharose affinity chromatography and the purified monoclonal antibody was conjugated with FITC and Immunofluorescence was done for confirming the specific binding. Conclusion: The conjugated monoclonal antibody could have application in diagnosis B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells.

Terminal Deoxynucleotidyl Transferase (TdT)Inhibition ofCord Blood Derived B and T Cells Expansion

Abstract: Purpose: Terminal deoxynucleotidyl transferase(TdT) is a DNA polymerase that is present in immature pre-B and pre-T cells. TdT inserts N-nucleotides to the V (D) J gene segment during rearrangements of genes, therefore, it plays a vital role in the development and variation of the immune system in vertebrates. Here we evaluated the relationship between cytokines like interleukin-2 (IL-2), interleukin-7 (IL-7), and interleukin-15 (IL-15) and TdT expression in cord blood mononuclear cells and also effect of inhibition in the expansion of B and T cells derived from cord blood. Methodes: The cord blood mononuclear cells were cultured with different combination of cytokines for 21days, which they were harvested in definite days (7, 14 and 21) and evaluated by flow cytometry. Results: Our data indicated that TdT expression increased in cord blood mononuclear cells using immune cell key cytokines without being dependent on the type of cytokines. TdT inhibition reduced both the expansion of B and T cells derived from cord blood and also declined the apoptosis and proliferation. Considered together, TdT played an important role in the control of the expansion of B and T cells derived from cord blood. Conclusion: considered together, it was observed that TdT expression was increased by cytokines and TdT inhibition not only reduced B and Tcells derived from cord blood, but it also affected the rate of apoptosis and proliferation.

Temporal and spatial evaluation of environmental noise in urban area: a case study in Iran

Abstract: Environmental noise pollution is a disrupting factor in the urban areas which can lead to adverse health effects, behavior and quality of life. Present study was carried out in Tabriz (Iran) and aimed to achieve a real condition of noise pollution. Thirty-five measurement stations were selected, and noise levels were recorded. The equivalent noise level in (A) frequency weighting network, route-mean-square sound pressure level, minimum sound pressure level, maximum sound pressure level and noise pollution level were computed as applicable indices. Temporal and spatial variability of these noise indices were plotted by Arc GIS. Public awareness about the noise pollution was assessed through interview. Mixed model and pairwise comparisons were used for comparison of noise indices based on stations and times. It was found that noise levels were significantly different and higher than permissible levels at most stations, especially at heavily travelled crossroads and squares. Mean equivalent noise level for morning, noon, afternoon and night was 71.35 ± 7.49, 71.63 ± 4.90, 70.67 ± 4.52 and 68.74 ± 5.22 dB(A), respectively. The noise indices of all stations and measurement times had significant difference (p value = 0.028 and 0.019, respectively). It was observed that in normal traffic, the highest sound levels are produced by buses, trucks and motorbikes due to low-technology engines. Using the horn was the first reflex of the most drivers. A main part of the noise pollution problem of the studied city can be attributed to driving culture and non-compliance with traffic laws.

Effect of Sixteen Weeks Combined Training on FGF-23, Klotho, and Fetuin-A Levels in Patients on Maintenance Hemodialysis.

Abstract: Introduction. Fibroblast growth factor 23 (FGF-23) and its cofactor alfa klotho, are one of the most important factors, directly and indirectly, involved in the process of calcification and atherosclerosis. This study aimed to evaluate the efficacy of a combination of regular exercise during dialysis on quality of life and markers including FGF-23, alfa klotho, and fetuin-A levels. Methods. Forty-five hemodialysis patients aged 61 ± 9.02 years and weight 69 ± 11.25 kg were randomly divided into two training, EX (n = 24) and control groups, CON (n = 21). The EX group patients participated in a 16-week combined aerobic and resistance exercise program during dialysis. Bone markers including, FGF-23, klotho, fetuin-A, were measured before and at the end of the study in both groups. Statistical analysis for comparing data change during study by SPSS software and the P value was set at .05. Results. In the control group in the secondary assessment, reduction in quality of life was observed (P .05), however significant rising of klotho was observed in treated patients (P < .05). Also, combined training reduced the amount of phosphorus, parathyroid hormone; significantly (P < .05). Conclusion. This study showed that regular exercise during dialysis improves quality of life and physical functions. No significant change in FGF-23 and CRP were observed during the study. However significant rising of klotho and reduction of iPTH and phosphorous levels were observed in treated patients.