Tata Memorial Hospital

About: Tata Memorial Hospital is a healthcare organization based out in Mumbai, India. It is known for research contribution in the topics: Cancer & Breast cancer. The organization has 3187 authors who have published 4636 publications receiving 109143 citations.

Multiplex Detection of Pediatric Low-Grade Glioma Signature Fusion Transcripts and Duplications Using the NanoString nCounter System.

Abstract: Previous studies identified recurrent fusion and duplication events in pediatric low-grade glioma (pLGG). In addition to their role in diagnosis, the presence of these events aid in dictating therapy and predicting patient survival. Clinically, BRAF alterations are most commonly identified using fluorescent in situ hybridization (FISH). However, this method is costly, labor-intensive and does not identify nonBRAF events. Here, we evaluated the NanoString nCounter gene expression system for detecting 32 of the most commonly reported fusion/duplication events in pLGG. The assay was validated on 90 pLGG samples using FISH as the gold standard and showed sensitivity and specificity of 97% and 98%, respectively. We next profiled formalin-fixed paraffin-embedded preserved biopsy specimens from 429 pLGG cases. 171 (40%) of the cases within our cohort tested positive for a fusion or duplication event contained within our panel. These events, in order of prevalence, were KIAA1549-BRAF 16;9 (89/171, 52.0%), KIAA1549-BRAF 15;9 (42/171, 24.6%), KIAA1549-BRAF 16;11 (14/171, 8.2%), FGFR1-TACC1 17;7 (13/171, 7.6%), MYBL1 duplication (5/171, 2.9%), KIAA1549-BRAF 18;10 (4/171, 2.3%), KIAA1549-BRAF 15;11 (2/171, 1.2%), FAM131B-BRAF 2;9 (1/171, 0.6%), and RNF130-BRAF 3;9 (1/171, 0.6%). This work introduces NanoString as a viable clinical replacement for the detection of fusion and duplication events in pLGG.

Results of surgical resection in pelvic Ewing's sarcoma.

Abstract: Objective To evaluate the results of patients with non-metastatic Ewing's sarcoma of the pelvis treated with surgical resection as part of their multimodality treatment Methods Twenty-six patients treated between September 2000 and September 2009 were evaluated. Thirteen resections included the acetabulum and 13 did not. Thirteen resections excluding the acetabulum had no reconstruction. Arthrodesis was done in two, extracorporeal radiation and reimplantation in two, and pseudarthrosis in nine patients. Results Three patients had involved margins. Seventeen patients had good response to chemotherapy and nine were poor responders. Twenty-one patients were available for follow-up. The follow-up ranged from 4 to 129 months (mean 36 months). Thirteen patients are currently alive. There was one local recurrence. On Kaplan–Meier analysis the overall survival was 72% at 5 years. The 3-year survival in good responders to chemotherapy was 94% compared to 30% in poor responders. The Musculoskeletal Tumor Society Score ranged from 23 to 29, with patients in whom the acetabulum was retained having better function compared to patients in whom acetabulum was resected. Conclusion Surgery provides good local control and oncologic outcomes with acceptable function in these patients. J. Surg. Oncol. 2012; 106:417–422. © 2012 Wiley Periodicals, Inc.

Integrated PET/CT in evaluating sarcomatous transformation in osteochondromas.

Abstract: Aim:To study the role of PET-CT in evaluating sarcomatous transformation in osteochondromas.Materials and Methods:This was a retrospective analysis of a prospective data base of 12 patients from 2005 to 2007 with a clinical diagnosis of an osteocartilaginous lesion who were referred for a FDG PET-CT