Showing papers by "Tehran University of Medical Sciences published in 2002"

An accelerated nutrition transition in Iran.

Abstract: Objective: To describe the emergence of the nutrition transition, and associated morbidity shifts, in the Islamic Republic of Iran. Design: Review and analysis of secondary data relating to the socio-political and nutritional context, demographic trends, food utilisation and consumption patterns, obesity, and diet-related morbidity. Results and conclusions: The nutrition transition in Iran is occurring rapidly, secondary to the rapid change in fertility and mortality patterns and to urbanisation. The transition is occurring against the backdrop of lack of sustained economic growth. There is considerable imbalance in food consumption with low nutrient density characterising diets at all income levels, over-consumption evident among more than a third of households, and food insecurity among 20% of the population. Obesity is an emerging problem, particularly in urban areas and for women, and both diabetes and other risk factors for heart disease are becoming significant problems.

Vitamin C function and status in chronic disease.

Abstract: ▪ Abstract Vitamin C is an essential dietary nutrient required as a co-factor for many enzymes, and humans are among the few animals that lack the ability to synthesize the compound from glucose. The reduced form of the vitamin, ascorbic acid, is an especially effective antioxidant owing to its high electron-donating power and ready conversion back to the active reduced form. Concentrations of the vitamin in body tissues and fluids are regulated through interactions of intestinal absorption, cellular transport, and excretion. The amount of vitamin C needed to prevent scurvy is very small and easily obtained in nearly all Western diets. There is great interest in the clinical roles of vitamin C because of evidence that oxidative damage is a root cause of, or at least associated with, many diseases. Population studies show that individuals with high intakes of vitamin C have lower risk of a number of chronic diseases, including heart disease, cancer, eye diseases, and neurodegenerative conditions. However, these results may simply reflect a more healthful diet or lifestyle for individuals with a high vitamin C intake. At present, data from controlled clinical trials have not established that higher intakes of vitamin C alone will help prevent chronic degenerative diseases. However, the evidence that ascorbic acid acts as an important antioxidant in many body tissues is convincing. The new higher Recommended Dietary Allowance (RDA) for vitamin C of 75 mg for women and 90 mg for men is, for the first time, based on the vitamin's role as an antioxidant as well as protection from deficiency. In healthy people, amounts greater than the RDA do not appear to be helpful. Vitamin C nutriture may be more important for people with certain diseases or conditions. High intakes of the vitamin are generally well tolerated; a Tolerable Upper Level was recently set at 2 g based on gastrointestinal upset that sometimes accompanies excessive intakes.

Induction of oxidative stress and acetylcholinesterase inhibition in organophosphorous pesticide manufacturing workers.

Abstract: Oxidative stress status and acetylcholinesterase (AChE) activity were studied in blood samples obtained from 45 organophosphorous (OP)-formulating pesticide workers with a minimum work history of 1 year in the age range of 23-55. Controls were age-matched workers of a food-making factory. They were evaluated for oxidative stress markers, including thiobarbituric acid-reactive substances (TBARS) indicator of lipid peroxidation (LPO), ferric-reducing ability of plasma (FRAP) indicator of total anti-oxidant capacity, total thiol (SH) groups and gamma glutamyl transpeptidase (GGT) levels in blood and AChE activity in erythrocytes. The results show marked inhibition of AChE activity, increased TBARS, decreased FRAP and decreased thiol group levels in workers. The reduction in activity of AChE correlated well with increased TBARS and decreased FRAP in OP formulators. It is concluded that OP-formulating workers are exposed to more oxidative stress. The measurement of erythrocyte AChE activity in pesticide workers who formulate OPs can be a good monitoring factor and is recommended to be performed in a regular manner.

Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran

Abstract: Iran is an important endemic focus of cystic hydatid disease (CHD) where several species of intermediate host are commonly infected with Echinococcus granulosus. Isolates of E. granulosus were collected from humans and other animals from different geographical areas of Iran and characterized using both DNA (PCR-RFLP of ITS1) and morphological criteria (metacestode rostellar hook dimensions). The sheep and camel strains/genotypes were shown to occur in Iran. The sheep strain was shown to be the most common genotype of E. granulosus affecting sheep, cattle, goats and occasionally camels. The majority of camels were infected with the camel genotype as were 3 of 33 human cases. This is the first time that cases of CHD in humans have been identified in an area where a transmission cycle for the camel genotype exists. In addition, the camel genotype was found to cause infection in both sheep and cattle. Results also demonstrated that both sheep and camel strains can be readily differentiated on the basis of hook morphology alone.

Hepatitis C risk factors in Iranian volunteer blood donors: A case–control study

Abstract: Background: In order to evaluate some possible risk factors for the spread of hepatitis C infection a case–control study was undertaken. Methods: The study population consisted of first time blood donors referred to the Iranian Blood Transfusion Organization. Enzyme-linked immunosorbent assay and recombinant immunoblot assay anti-hepatitis C virus (HCV) tests were performed for all samples. Risk factors were evaluated using a questionnaire. A stepwise forward logistic regression analysis was used. Results: The case group consisted of 193 HCV-positive donors, and 196 HCV-negative donors comprised the control group. Transfusion, undergoing endoscopy, extramarital sexual activities, non-intravenous (i.v.) drug abuse, i.v. drug abuse, and receiving wounds at war were found to be independent risk factors of being HCV-positive (Odds ratio: 17, 4, 42.2, 34.4, 52.8 and 5.2, respectively). No apparent risk factors could be demonstrated in 24.5% of the positive cases. Conclusion: This study could be of particular interest because of the low seroprevalence of hepatitis C and the religious culture of the study population. There are certain medical procedures, lifestyle patterns, and customs and cultural matters in Iran that predispose people to a number of HCV risk factors.

Effects of intrahippocampal injection of GABAergic drugs on memory retention of passive avoidance learning in rats.

Abstract: The effect of post-training intrahippocampal injection of gamma-aminobutyric acid (GABA) receptor agonists and antagonists, immediately after a training session on memory retention of passive avoidance learning in rats, was measured in the presence and absence of physostigmine. Post-training treatments were carried out in all the experiments. The different doses of the GABAA receptor agonist muscimol (2, 4 and 6 microg/rat) decreased memory retention in rats dose-dependently. The higher response was obtained with 6 microg/rat of the drug. When the GABAA receptor antagonist bicuculline (0.5, 1, 2 and 4 microg/rat) was administered, only one dose of the drug (1 microg/rat) increased memory retention; however, the antagonist reduced the effect of muscimol. The GABAB receptor agonist, baclofen (0.25, 0.5, 1 and 2 microg/rat) also reduced memory retention in the animals. Intrahippocampal injection of lower doses of the GABAB receptor antagonist CGP35348 (P-[3-aminopropyl]-p-diethoxymethyl-phosphinic acid) (2.5, 5, 10 microg/rat) did not effect memory retention, although the higher doses of the drug (25 and 50 microg/rat) decreased memory retention. The doses of antagonist (2.5, 5 and 10 microg/rat), which did not elicit any response alone, reduced the effect of baclofen. The inhibitory response of CGP35348 was also decreased by bicuculline. In another series of experiments, physostigmine improved memory retention. The GABA receptor agonists, muscimol and baclofen, as well as the GABA receptor antagonists bicuculline and CGP35348, decreased the effect of physostigmine. Atropine decreased memory retention by itself and potentiated the response of muscimol and baclofen. It is concluded that GABAA and GABAB receptor activation may be involved in the impairment of memory retention.

Primary immunodeficiency in Iran: first report of the National Registry of PID in Children and Adults.

Abstract: Epidemiological studies have shown wide geographical and racial variation in the prevalence and patterns of immunodeficiency disorders. To determine the frequency of primary immunodeficiencies (PID) in Iran, the Iranian Primary Immunodeficiency Registry (IPIDR) was organized in 1999. We extracted the patient's data, by using a uniform questionnaire from their hospital records. The diagnosis of patients was based on WHO criteria. By now, 440 patients with PID, who were observed during a period of 20 years, have been registered in our registry. Among these patients, the following frequencies were found: predominantly antibody deficiency in 45.9% of patients (n = 202), phagocytic disorders in 29.09% (n = 128), T-cell disorders in 24.31% (n = 107), and complement deficiencies in 0.68% (n = 3). Common variable immunodeficiency was the most frequent disorder (n = 98), followed by chronic granulomatous disease (n = 86), ataxia telangiectasia (n = 48), x-linked agammaglobulinemia (n = 45), selective IgA deficiency (n = 42), combined immunodeficiency (n = 15), and severe combined immunodeficiency (n = 14). This study revealed that antibody deficiencies is the most frequently diagnosed primary immunodeficiency disorder in our patients, which is similar to that observed in other registries. A comparative study shows some differences between our results and other registries.

Initial nasolacrimal duct probing in children under age 5: cure rate and factors affecting success.

Abstract: Aims: To evaluate the success rate of initial probing and the factors affecting the success rate for congenital nasolacrimal duct obstruction (CNLDO)in children under the age of 5 years. Methods: In a prospective uncontrolled interventional case series, 207 eyes from 161 consecutive patients with CNLDO underwent nasolacrimal duct probing under brief general anesthesia. Based on exclusion criteria, 180 eyes from 139 patients were included in the study. Diagnoses were made according to the patients' histories, clinical examinations, and modified dye disappearance tests. The state of the punctum, the canaliculus, the sac, and the nasolacrimal duct were recorded. The main outcome measure was the complete disappearance of tearing and discharge in the affected eye. The patients were followed at 1 week, 1 month, and 3 months postoperatively. Results: The age range was 5 to 60 months (mean, 19.1 ± 11.2 months). The cure rates were 92% in the first year, 84.5% in the second year, 65% in the third year, and 63.5 in the fourth and fifth years of age. The patient's age, nonmemberanous CNLDO, and canalicular stenosis were correlated with the success of initial nasolacrimal duct probing ( P Conclusions: Based on the results, simplicity of probing, and absence of significant complications, initial nasolacrimal duct probing is advised up to the age of 5 years. Increasing age, nonmembranous CNLDO, and canalicular stenosis increase the failure rate ( P

A new focus of cutaneous leishmaniasis caused by Leishmania tropica.

Abstract: OBJECTIVES To determine the epidemiological status of the cutaneous leishmaniasis outbreak, isolation and identification of the parasite and preparation of a control program in a recently identified focus located in the Province of Yazd, Central Iran. METHODS This study was conducted in 124 classrooms of primary school children and among 139 households of an infected quarter in the city of Yazd during 1999-2000. All the students and members of the households were questioned and examined for the presence of ulcer(s) or scar(s). For each case a form was completed and necessary information were recorded. Smears were prepared from the edge of the ulcer and examined for the presence of amastigotes. Leishmania stocks from human lesions were isolated. Cultured promastigotes were identified using Random Amplified Polymorphic DNA-Polymerase Chain Reaction. Stray dogs were shot in the city and examined for the presence of any ulcer(s) or scar(s). RESULTS An endemic focus of cutaneous leishmaniasis caused by Leishmania tropica was detected in Yazd City, Central Iran. Examination of 3176 students aged 6-16 years old in 124 primary schools showed a rate of 0.7% for scars and 0.2% for active lesions. There was no significant differences between the sexes in the prevalence of active lesions or scars. A study of prevalence among 139 households with a total population of 572 persons in Darvazeh Ghassabha quarter showed a prevalence of 13.5% for scars and 0.5% for ulcers. The most highly infected age group was 5-9 with a rate of 1.8%. Males and females were equally infected. Forty-six stray dogs were examined and none of them appeared to be infected. CONCLUSIONS According to this study, cutaneous leishmaniasis due to Leishmania tropica is endemic in the city of Yazd, Central Iran. Humans are considered to be the main reservoir and the transmission is believed to be from human to human by Phlebotomus sergenti. This focus now can be added to the list of anthroponotic cutaneous leishmaniasis foci in the country. Any residual insecticide spraying is not recommended during the next year due to the low prevalence of the disease.

Involvement of dopamine D2 receptors of the central amygdala on the acquisition and expression of morphine-induced place preference in rat.

Abstract: In the present study, the effects of intra-central amygdala (CeA) injections of dopamine (DA) D2-like receptor agonist and antagonist on the acquisition and expression of morphine-induced place preference in male Wistar rats have been investigated. Subcutaneous administration of different doses of morphine sulphate (0.5-10 mg/kg) produced a dose-dependent conditioned place preference (CPP). Using a 3-day schedule of conditioning, it was found that the DA D2/D3 receptor agonist, quinpirole (0.3-3 microg/rat), or the DA D2 receptor antagonist, sulpiride (0.04-5 microg/rat), did not produce a significant place preference or place aversion. Intra-CeA administration of quinpirole (0.3 and 1 microg/rat) with an ineffective dose of morphine (0.5 mg/kg) elicited a significant CPP. On the other hand, quinpirole (0.3 microg/rat) injection into the CeA induced CPP in combination with the lower doses of morphine (0.5 and 2.5 mg/kg), but decreased the response of higher dose (7.5 mg/kg) of morphine. This response of quinpirole was attenuated by sulpiride (0.2 microg/rat). Sulpiride by itself (0.04-5 microg/rat) reduced the acquisition of morphine (7.5 mg/kg)-induced place preference. The administration of the higher dose of sulpiride (1 and 5 microg/rat) or the higher dose of quinpirole (3 microg/rat) during acquisition decreased the locomotor activity of the animals on the testing days. The injection of the low dose of quinpirole (0.3 microg/rat) on the test day reduced the expression of morphine-induced CPP, but the high dose of quinpirole (3 microg/rat) potentiated this expression. The administration of sulpiride (5 microg/rat) attenuated the quinpirole response. The injection of sulpiride (1 and 5 microg/rat) abolished the expression of morphine-induced CPP. It is concluded that the CeA DA D2-like receptors may play an active role in morphine reward.

Symptoms of Obsessive-Compulsive Disorder in a Sample of Iranian Patients:

Abstract: Background: Characteristic features of the obsessive-compulsive disorder (OCD) occur with remarkable consistency in different cultural settings. The content of symptoms, however, seems to vary acro...

Effect of imipramine on the expression and acquisition of morphine-induced conditioned place preference in mice.

Abstract: The effect of imipramine and α-adrenoceptor agonists and antagonists on the acquisition or expression of morphine-induced conditioned place preference (CPP) was studied in mice. An unbiased CPP paradigm was used to study the effect of the agents. In the first set of experiments, the drugs were used during the development of CPP by morphine or they were used alone in order to see if they induce CPP or conditioned place aversion (CPA). Our data showed that intraperitoneal injection of morphine sulphate (2.5–10 mg/kg) induced CPP in mice. Imipramine (0.5–2.5 mg/kg), phenylephrine (0.5–2 mg/kg), yohimbine (0.5–2 mg/kg) or prazosin (0.1–1 mg/kg) did not influence CPP, but clonidine (0.002–0.05 mg/kg) induced CPA. Yohimbine increased, while clonidine and prazosin reversed, morphine-induced CPP. Phenylephrine did not influence the CPP induced by morphine. In the second set of experiments, when the drugs were used before testing on Day 6, in order to test their effects on the expression of morphine-induced CPP, imipramine (0.5–5 mg/kg) reversed morphine-induced CPP and this reversal was blocked by naloxone (2 mg/kg). Clonidine and prazosin reversed, while yohimbine decreased morphine-induced CPP. Phenylephrine did not alter the morphine response. Furthermore, yohimbine and prazosin reversed the imipramine effect. None of the drugs influenced locomotion. However, prazosin or yohimbine in combination with morphine altered locomotor activity during the acquisition of CPP. Yohimbine by itself increased locomotion. It is concluded that imipramine can induce CPA through an opioid receptor mechanism and α-adrenoceptor agents may influence morphine CPP.

Diminished Th1 and Th2 cytokine production in healthy adult nonresponders to recombinant hepatitis B vaccine.

Abstract: Vaccination of healthy adults with recombinant hepatitis B (rHB) vaccine fails to induce a protective antibody response in a proportion of individuals. Imbalanced T-helper (Th)1/Th2 response has been attributed to the lack of specific antibody response to rHB vaccine. In this study, in vitro production of interleukin (IL)-2, interferon (IFN)-γ and IL-10 was investigated in Iranian healthy adults vaccinated with rHB vaccine. Peripheral blood mononuclear cells (PBMC) were isolated from 18 high responders and eight nonresponders and stimulated with rHB antigen or phytohaemaglutinin (PHA) mitogen. The cytokines were quantitated in culture supernatants by sandwich enzyme-linked immunosorbent assay (ELISA). Our results demonstrated a significant decrease in the production of IL-2, IFN-γ and IL-10 (P < 0.005) in response to rHB antigen. The levels of all cytokines induced by PHA were similarly represented in both groups of vaccinees. These findings suggest that unresponsiveness to rHB vaccine may be owing to inadequate Th1- and Th2-like cytokine production.

Radioprotective Effects of 2-Imino-3-[(chromone-2-yl)carbonyl] thiazolidines against γ-Irradiation in Mice

Abstract: A series of 2-imino-3-[(chromone-2-yl) carbonyl]thiazolidines substituted at the C-5 and/or C-7 positions of a chromone ring were synthesized. The in vivo toxicity and radioprotective efficacy of these agents were evaluated in male NMRI mice against cobalt-60 gamma-rays. The LD50 values as determined by a Probit analysis, were 659, 1216 and 790 mg/kg for compounds, 2, 3 and 4, respectively. For studying radioprotective effects, one half of the toxic LD50 values were used, namely 330, 605 and 395 mg/kg for compounds 2, 3 and 4, respectively. The dose reduced factor (DRF) was determined by dividing the LD50/30 values obtained from the radiation survival curve in the presence of a radioprotective agent by the LD50/30 value obtained from a control radiation survival curve. A compound with a hydroxyl group substituent at the C-5 position afforded better radioprotective activity than those without this substituent. The radioprotective effect of chromone having a hydroxyl group at only the C-7 position was similar to that of the unsubstituted chromone. The most active compound has hydroxyl groups at the C-5 and C-7 positions of the chromone ring; it had a DRF of 1.48.

An Iranian family with Alzheimer's disease caused by a novel APP mutation (Thr714Ala).

Abstract: Despite the fact that APP mutations were the first described cause of AD,1 relatively few pathogenic mutations have been described. In this article, we describe the occurrence of a novel APP mutation (T714A) in an Iranian family with nine documented affected individuals in three generations. The phenotype in this family is similar to that of many of the other families with APP mutations, with an average onset age of approximately 55 years. A family with multiple members affected by progressive dementia was identified in northern Iran (see the figure). The average age at onset of dementia was in approximately 55 years with a disease progression lasting 2 to 10 years. The clinical features and age at onset were reminiscent of the first family described with APP mutations.1,2⇓ We briefly describe the clinical features of the family and the identification of the pathogenic mutation and discuss the …

Dopamine receptor mechanism(s) and morphine tolerance in mice.

Abstract: Based on our previous demonstration of the involvement of dopamine-2 (D2) dopamine receptors in morphine antinociception, we examined the role of D2 dopamine receptors in the expression and development of tolerance to morphine antinociception in mice. Tolerance to morphine antinociception was assessed by the tail-flick response after the administration of morphine (50 mg/kg) once daily for 3 days. The D2 dopamine receptor agonist, quinpirole (0.01, 0.02 and 0.03 mg/kg), but not the D2 dopamine receptor antagonist, sulpiride (12.5, 25 and 50 mg/kg), increased morphine antinociception in morphine non pre-exposed mice. The response of quinpirole was decreased by the lower doses of sulpiride. Both quinpirole and sulpiride decreased the expression and development of tolerance to antinociception induced by morphine (1.5, 3 and 6 mg/kg). The effect of quinpirole on the expression and development of tolerance, was reduced by a lower and per se non-effective dose of sulpiride. It was concluded that D2 dopaminergic receptors may play a part in the expression and development of tolerance to the antinociceptive effect of morphine.

Three major glucose-6-phosphate dehydrogenase-deficient polymorphic variants identified in Mazandaran state of Iran.

Abstract: Summary. We report the first investigation of glucose- 6-phosphate dehydrogenase (G6PD) deficiency among the Mazandaranians in the north of Iran. We analysed the G6PD gene in 74 unrelated G6PD-deficient men with a history of favism. Molecular analysis revealed three major different polymorphic variants: G6PD Mediterranean 66·2% (49 out of 74), G6PD Chatham 27% (20 out of 74), G6PD Cosenza 6·75% (5 out of 74). These findings indicated a higher prevalence of G6PD Chatham in this Iranian population than anywhere else in the world. In addition, the distribution of these G6PD variants is more similar to that found in an Italian population than in other Middle Eastern countries.

Composition of the Essential Oil of Bunium persicum (Boiss.) B. Fedtsch. from Iran

Abstract: The chemical composition of the essential oil obtained from the fruits of Bunium persicum was examined by using GC and GC/MS Among the 25 components identified in this oil, cuminaldehyde (270%), γ-terpinene (258%), p-cymene (121%), cuminyl alcohol (60%) and limonene (51%) were found to be the major constituents