Showing papers by "Temple University published in 1968"
TL;DR: Findings, as well as other evidence, indicate that one of the compounds is guanidino-methylated arginine and the other is α-amino-, guanIDino- methylatedArginine.
204 citations
TL;DR: This paper describes several operational factors which lend themselves to more objective assessment and therapeutic maneuvers and three treatment techniques which can presumably lead to effective and specific treatment procedures.
183 citations
TL;DR: Commercial serum albumin was found to contain lactate, pyruvate, and especially citrate in addition to fatty acids, andCharcoal treatment followed by prolonged dialysis was effective in removing most of these contaminants.
141 citations
TL;DR: A modification of the Park and Johnson procedure for reducing sugar determination is described which permits determination of the reducing power of insoluble materials such as bacterial cell wall components.
92 citations
TL;DR: The biosynthetic enzyme or enzymes displayed a relatively high degree of specificity for UDP-glucose (the best of five nucleoside diphosphate glucose substrates tried) and for the 1,2 stereoconfiguration of the diglyceride substrate.
68 citations
TL;DR: The data support the hypothesis that loss of tone and loss of contractile activity in the large arterial vessels following denervation produce an increase of tone in the smaller, more distally located vessels, thereby causing a redistribution of the site of regulation of flow through capillary nets.
Abstract: Previous studies of microcirculation in the denervated wing of the unanesthetized bat showed that major arterial vessels increased markedly in diameter and lost their ability to contract spontaneously Arteriolar vessels did not show such an increase in diameter, and furthermore their spontaneous contractile activity increased These observations led to the idea that regulation of blood flow and blood pressure might be continued to some degree in the absence of central nervous control through enhanced activity of arteriolar vessels which just precede the capillary nets
Therefore a thorough study by direct microscopic observation of the behavior of arterial vessels in the following surgical and pharmacological denervation was made The data support the hypothesis that loss of tone and loss of contractile activity in the large arterial vessels following denervation produce an increase of tone and activity in the smaller, more distally located vessels, thereby causing a redistribution of the site of regulation of flow through capillary nets The largest arterial vessels showed and increase in average diameter of 38% after surgical denervation, while the terminal arterioles showed a decrease in average diameter of 24% Neither phenoxybenzamine (Dibenzyline) norpentolinium (Ansolysen) were as effective as surgical denervation in producing relaxation of the large arterial vessels Neither drug inhibited spontaneous contractile activity of the terminal vessels
65 citations
63 citations
TL;DR: The difficulties of management and diagnosis are illustrated and multiple papillomatosis is established as a definite clinicopathologic entity.
Abstract: Eleven cases of multiple papillomatosis of the lower respiratory tract are reported and the clinicopathologic picture is studied. These cases include some extremely rare varieties and a 44‐year follow‐up. When the disease starts during childhood, it usually appears to be self‐limiting if properly managed. In adults, however, the disease sometimes runs a more protracted course with a higher risk of developing cancer. This study illustrates the difficulties of management and diagnosis and establishes the disease as a definite clinicopathologic entity.
60 citations
58 citations
TL;DR: The natural response of healing tissue to an implanted foreign body is to encapsulate it in scar, which represents a modification of the natural process of tissue repair only in that continuity of tissue is never fully restored as long as the implant remains to occupy space which would otherwise be filled by scar.
Abstract: The natural response of healing tissue to an implanted foreign body is to encapsulate it in scar. This represents a modification of the natural process of tissue repair only in that continuity of tissue is never fully restored as long as the implant remains to occupy space which would otherwise be filled by scar. All natural cavities, exposed surfaces or channels in the body are normally lined with highly specialized tissues adopted to certain roles, thus : epithelial, endothelial and mucosal surfaces. When the integument or the walls of hollow organs are injured, their repair consists of restoration of structural integrity by connective-tissue-scar formation. However, where scar bridges a gap at an exposed surface, simple connective tissue does not remain bare; it is always covered by new surface tissue which has the histologic characteristics of the adjacent normal epithelium, mucosa or endothelium. These specialized surface “finishes” are the protective coating which permits the underlying, supporting tissues to attain metabolic stability, free from the infection and irritation to which they are susceptible. Such surface tissues, the “finishing touch” of the healing process, halt fibrin deposition, proliferation of ‘granulation tissue and development of more
54 citations
TL;DR: An amino acid-incorporating system which has very high endogenous activity has been obtained from insect pupae and a 5-fold increase in the ratio of tryosine incorporation to leucine incorporation was observed, suggesting control of cuticular protein synthesis at the translation level.
TL;DR: The hypothesis that certain of the biological activities induced by endotoxins are mediated via the complement system is supported by experiments conducted on LPS isolated from Serratia marcescens, Veillonella alcalescens, and Salmonella typhosa.
Abstract: Endotoxic lipopolysaccharides (LPS) isolated from Serratia marcescens, Veillonella alcalescens, and Salmonella typhosa were potent in their ability to induce fixation of complement (C') in normal guinea pig, rabbit, mouse, and human serum. The C'-fixing ability of LPS was pronounced even when assays were performed in undiluted serum, and was lost after each of four chemical modifications which resulted in loss of biological toxicities. The detoxification procedures had in common the cleavage of ester-bound, long-chain carboxylic acids. The ability of biologically active LPS to fix C' in normal guinea pig serum was reflected chiefly in dramatic uptake of classical C'3 (C'3t); fixation of C'1, C'4, and C'2 was virtually undetectable. Hence, it was the capacity for fixation of C'3t which was lost most overtly during detoxification. Addition of immune serum to the assay mixtures resulted in detectable fixation of C'1 and C'4. Biologically active LPS also fixed more of these components than did detoxified LPS. Immune serum restored the ability of detoxified LPS to fix C'3t, but whether this is by the original pathway is not yet clear. We concluded that the loss of certain biological activities and the loss of ability to fix C'3t in normal serum after LPS detoxification involved loss or rearrangement of substrates on LPS which either initiated or supported, or both, its interaction with the complement system. It was apparent that the ability to fix C' can serve as a valuable in vitro indicator of the integrity of the toxic conformation of biologically active LPS membrane fragments. These experiments supported the hypothesis that certain of the biological activities induced by endotoxins are mediated via the complement system.
TL;DR: In this article, the teratogenic potential of morphine sulfate is investigated in mice and it is shown that high subcutaneous doses, 100 to 500 mg/kg, administered to CF-1 mice on Day 8 or Day 9 of gestation, result in a large number of fetuses with exencephaly and axial skeletal fusions.
TL;DR: An extracellular protease of the dermatophyte Trichophyton granulosum, used in the initial digestion of hair substrates, has been isolated from the culture filtrate and an amino acid analysis indicates a large tryptophan content.
TL;DR: Pertinent literature dealing with other idiopathic osteolytic processes is discussed and a distin of essential osteolysis with nephropathy is presented.
Abstract: 1. The literature pertaining to essential osteolysis with nephropathy is reviewed.2. A case of essential osteolysis with nephropathy is presented with roenitgenographic and histological documentation.3. Pertinent literature dealing with other idiopathic osteolytic processes is discussed and a distin
TL;DR: The evidence reported here indicates that RNA enters into the cells as an undegraded molecule.
Abstract: SummaryThe entrance of RNA into ascites cells has been studied using double labeled rat liver RNA and high resolution electron microscope autoradiography. The evidence reported here indicates that RNA enters into the cells as an undegraded molecule.
TL;DR: The results are consistent with the previously postulated location of active autoly sin at the areas of new wall synthesis and the random location of latent autolysin in LOG walls.
Abstract: Cell walls (LOG walls) were isolated from cultures of Streptococcus faecalis ATCC 9790 in the exponential phase of growth. These walls were either allowed to undergo autolytic dissolution (in the presence or absence of trypsin) or wall autolysis was inactivated with sodium dodecylsulfate (SDS walls). Inactivated walls were treated either with lysozyme or with isolated, partially purified S. faecalis autolysin. During wall lysis, samples were removed, negatively stained with phosphotungstate, and examined in the electron microscope. Both lysozyme and isolated autolysin appeared to act over the entire surface of SDS walls. After partial dissolution, a fibrous network over the surface was revealed. Lysozyme digestion revealed the presence of prominent, highly-contrasted equatorial and subequatorial bands around the walls. After trichloroacetic acid extraction, the bands were seen less frequently and less distinctly in the partially lysozyme digested walls, suggesting that the bands contained nonpeptidoglycan polymers. In the absence of trypsin (which activates a latent form of the autolysin), autolysis of LOG walls appeared to start at the equatorial bands and to proceed back towards the apex of the coccus. Ribbons of wall material coming off the wide edge of the nearly hemispherical wall fragments were observed. Activation of latent autolysis resulted in lytic action over the entire wall surface. The results are consistent with the previously postulated location of active autolysin at the areas of new wall synthesis and the random location of latent autolysin in LOG walls.
TL;DR: Sotalol would be safe to use in those clinical states in which beta adrenergic blockade is indicated but cardiac depressant drugs are contraindicated, and if it can be verified that oxygen consumption is reduced, then cardiac efficiency would be increased by Sotalol.
Abstract: Sotalol [MJ 1999-dl-4(2-isopropylamino-1-hydroxylethyl) methanesulphonanilide] is a highly selective beta adrenergic receptor blocking agent devoid of major toxic effects in the isolated and intact myocardium of experimental animals. This study was performed to determine whether Sotalol acts as a beta adrenergic receptor blocking agent when given intravenously to man, the dose at which it blocks the beta adrenergic stimulating effect of an intravenous infusion of isoproterenol, the duration and constancy of its action and whether toxic effects would appear when the drug had been used in doses two to three times larger than the effective beta adrenergic blocking dose in man. Three healthy volunteers were utilized in an acute study to determine the beta adrenergic receptor blocking dose and 5 healthy volunteers were utilized in a chronic study to determine the constancy of action and possible toxic effects. Sotalol, in doses of 0.06 to 0.08 mg./kg., produced effective blockade of the isoproterenolinduced stimulation of the beta adrenergic receptors. These effects remained constant for five days, the duration of the chronic study. The drug, in doses two to three times larger than that required to inhibit the chronotropic action of isoproterenol, was well tolerated and produced no untoward or toxic effects in either the acute or chronic study. Sotalol, in doses sufficient to block the isoproterenol-induced beta adrenergic receptor stimulation, did not reduce cardiac output or cardiac work below control values. If it can be verified that oxygen consumption is reduced, then cardiac efficiency would be increased by Sotalol. Sotalol would thus be safe to use in those clinical states in which beta adrenergic blockade is indicated but cardiac depressant drugs are contraindicated.
TL;DR: The events which occur after reaggregation of dissociated embryonic chick thyroid cells are described in terms of a possible realtionship between lytic activity and the reestablishment of thyroid function after dissociation.
Abstract: The events which occur after reaggregation of dissociated embryonic chick thyroid cells are described. Although the cells rearrange themselves into follicles within a few hours after being packed by centrifugation, their complex cytoarchitecture is not maintained. The first sign of an impending change is the accumulation of large numbers of dense bodies in the apical cytoplasm. Shortly thereafter other types of lytic bodies become numerous, and blebs containing cytoplasmic membranous components appear at the apical cell surfaces. A short time later the basal cytoplasm contains large residual bodies concomitant with a reduction in the extent of the canalicular endoplasmic reticulum and an increase in the number of free ribosomes. With the passage of time there is a progressive decrease in the size and number of residual bodies, and the channels of rough endoplasmic reticulum reappear until the normal thyroid pattern is restored. Lytic phenomena coupled with vesiculation of the endoplasmic reticulum have been linked with control of function in several organs. Therefore, the results are discussed in terms of a possible realtionship between lytic activity and the reestablishment of thyroid function after dissociation.
TL;DR: The authors argued that there can be no permanent peace without a world state, and that a sense of world community cannot form unless national decision-makers resolve or ameliorate world tensions through a return to a wise diplomacy.
Abstract: Hans Morgenthau's political realism has led him to the conclusion that there can be no permanent peace without a world state. But, he says, there can be no world government until there is world community, and a sense of world community cannot form unless national decision-makers resolve or ameliorate world tensions through a return to a wise diplomacy.
TL;DR: After a second injection of IPR, DNA synthesis is depressed until high levels of glycogen are restored, since any induced decrease in peak glycogen concentration results in a decreased incorporation of 3HTdR into DNA.
TL;DR: A method employing non‐toxic doses of diaminoacridines combined with ultrafreezing and drying techniques, fluorescent microscopy, and fluorometry has been used in this study of hematic and fluid barriers in the vitreal chamber and surrounding tissues.
Abstract: A method employing non-toxic doses of diaminoacridines combined with ultrafreezing and drying techniques, fluorescent microscopy, and fluorometry has been used in this study of hematic and fluid barriers in the vitreal chamber and surrounding tissues.
These studies have demonstrated: that diaminoacridine concentrations of 3.38 × 10−6 M in the circulating blood failed to penetrate into the retina, vitreous body, and optic nerve head; that there are hematic barriers preventing the penetration of diaminoacridines into these tissues and fluids; that the barriers are located in the pigment epithelium, inner layer of ciliary epithelium, iridial retina, and vascular endothelium of retinal and optic nerve vessels; and that the precise anatomical sites of these barriers are given by (a) the part of the cell membrane of ciliary epithelium and iridial retina facing the posterior eye chamber, (b) the part of the cell membrane of pigment epithelium facing the retina, and (c) the part of the endothelial cell membrane of retinal and optic nerve head vessels facing the vascular lumen.
Injections in the vitreal or aqueous chambers to produce 3.38 × 10−6 M diaminoacridine concentrations inside either chamber have demonstrated that the hematic barriers which prevent the penetration of diaminoacridines from the blood into the retina and vitreous permit their passage in the opposite direction. The diaminoacridine solutions follow special patterns in their elimination from the vitreous of different species according to the type of retinal vascularization.
TL;DR: Cell cycle times were drastically shortened by transplantation and transplantation caused a transient delay of cells in G2 followed by a rapid acceleration and produced an immediate increase in the number of Cells in DNA synthesis by about 5–8%.
Abstract: 1
Ehrlich ascites tumor cells collected from donor mice on the 5th day after inoculation were injected into the peritoneal cavity of new recipient mice.
2
Cell cycle times were drastically shortened by transplantation, for instance, the length of the cell cycle from 47 to 21.5 hr, and the duration of S from 26.5 to 16.5 hr.
3
Transplantation also caused a transient delay of cells in G2 followed by a rapid acceleration and produced an immediate increase in the number of cells in DNA synthesis by about 5–8%.
TL;DR: A 2 × 2 design was employed; 70 undergraduates from two classes were exposed to complexity-simplicity and symmetry-asymmetry and showed an overwhelming preference for the complex-symmetrical shapes.
Abstract: A 2 × 2 design was employed; 70 undergraduates from two classes were exposed to complexity-simplicity and symmetry-asymmetry. Each class showed an overwhelming preference for the complex-symmetrica...
TL;DR: In this article, no significant changes in electron spin resonance patterns (neither signal amplitudes nor shapes) vs. age were found, although four classes of signals were observed, contrary to some crude notions of how genetic damage from aging of seeds might arise from free radical reactions.
TL;DR: In this article, certain derivatives of diazines containing the ferroin (NCCN) group have been prepared for chelation with metal ions, with the idea of providing new compounds capable of chelation.
TL;DR: This finding as well as those presented above for the adult and foetal rat liver are interpreted on the basis of a competition between phosphoenolpyruvate carboxykinase and NAD-malate dehydrogenase for oxaloacetate produced by the cleavage of citrate in the cytosol.
Abstract: 1. Foetal rat liver slices incorporate the C-3 of aspartate and C-2 of glutamate into fatty acids at rates equal to those observed with adult rat liver slices. Incorporation of either of these labelled carbon atoms into fatty acids would require a functioning citrate-cleavage pathway which consists of the enzymes ATP–citrate lyase, NAD–malate dehydrogenase and NADP–malate dehydrogenase. However, NADP–malate dehydrogenase is present in foetal rat liver at only 5% of the activity detectable in adult rat liver. 2. From these findings and the effect of cofactors on the formation of 14CO2 from [1,5-14C2]citrate in liver supernatant fractions (100000g), it is suggested that NADP–malate dehydrogenase limits the citrate-cleavage sequence. 3. Measurement of the citrate-cleavage pathway by incorporation studies with [3-14C]aspartate and [U-14C]glucose and by determining the activities of ATP–citrate lyase and NADP–malate dehydrogenase have shown that this sequence of reactions is present in the liver of the bovine foetus but not in the adult. However, C-2 of glutamate is not incorporated into fatty acids or non-saponifiable lipid by bovine liver slices. This finding as well as those presented above for the adult and foetal rat liver are interpreted on the basis of a competition between phosphoenolpyruvate carboxykinase and NAD–malate dehydrogenase for oxaloacetate produced by the cleavage of citrate in the cytosol.