Institution
The Chinese University of Hong Kong
Education•Hong Kong, China•
About: The Chinese University of Hong Kong is a education organization based out in Hong Kong, China. It is known for research contribution in the topics: Population & Computer science. The organization has 43411 authors who have published 93672 publications receiving 3066651 citations.
Topics: Population, Computer science, Cancer, Medicine, China
Papers published on a yearly basis
Papers
More filters
••
TL;DR: The authors investigated the effects of intragroup and cross-subgroup communications in an experimental field study on demographic faultlines and found that faultlines explained more variance in perceptions of team learning, psychological safety, satisfaction, and expected performance than single-attribute heterogeneity indexes.
Abstract: We investigated the effects of intragroup and cross-subgroup communications in an experimental field study on demographic faultlines. The results indicated that faultlines explained more variance in perceptions of team learning, psychological safety, satisfaction, and expected performance than single-attribute heterogeneity indexes. In addition, cross-subgroup work communications were effective for groups with weak faultlines but not for groups with strong faultlines. Overall, this study extends the original faultline model, documents the utility of the concept of faultlines, and identifies some of their effects on work group outcomes.
711 citations
••
New England Biolabs1, New York State Department of Health2, Columbia University3, Wayne State University4, University of Basel5, University of Toledo6, University of Edinburgh7, University of Alabama8, University of Portsmouth9, Moscow State University10, University of Illinois at Chicago11, University of Bristol12, University of Rochester13, Duke University14, University of Sheffield15, Vilnius University16, University of Giessen17, University of Copenhagen18, Hungarian Academy of Sciences19, North Carolina State University20, University of Tokyo21, Humboldt University of Berlin22, Brookhaven National Laboratory23, University of Massachusetts Medical School24, National Institutes of Health25, Indian Institute of Science26, University of Warsaw27, University of California, Santa Barbara28, State Research Center of Virology and Biotechnology VECTOR29, University of Oregon30, The Chinese University of Hong Kong31, University of Maryland, College Park32, Fred Hutchinson Cancer Research Center33, University of Wisconsin-Madison34, University of Nebraska–Lincoln35, University of Lisbon36
TL;DR: In this article, a nomenclature for restriction endonucleases, DNA methyltransferases, homing endon nucleases and related genes and gene products is described.
Abstract: A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes.
710 citations
••
TL;DR: Whether implementing additional “train-low” strategies to increase the training adaptation leads to enhanced performance in well-trained individuals is unclear.
Abstract: An athlete’s carbohydrate intake can be judged by whether total daily intake and the timing of consumption in relation to exercise maintain adequate carbohydrate substrate for the muscle and central nervous system (‘‘high carbohydrate availability’’) or whether carbohydrate fuel sources are limiting for the daily exercise programme (‘‘low carbohydrate availability’’). Carbohydrate availability is increased by consuming carbohydrate in the hours or days prior to the session, intake during exercise, and refuelling during recovery between sessions. This is important for the competition setting or for high-intensity training where optimal performance is desired. Carbohydrate intake during exercise should be scaled according to the characteristics of the event. During sustained high-intensity sports lasting *1 h, small amounts of carbohydrate, including even mouth-rinsing, enhance performance via central nervous system effects. While 30–60 g h 71 is an appropriate target for sports of longer duration, events42.5 h may benefit from higher intakes of up to 90 g h 71 . Products containing special blends of different carbohydrates may maximize absorption of carbohydrate at such high rates. In real life, athletes undertake training sessions with varying carbohydrate availability. Whether implementing additional ‘‘train-low’’ strategies to increase the training adaptation leads to enhanced performance in well-trained individuals is unclear.
709 citations
••
TL;DR: GTWR was compared with global ordinary least squares, TWR, and GWR in terms of goodness-of-fit and other statistical measures using a case study of residential housing sales in the city of Calgary, Canada, from 2002 to 2004 and showed substantial benefits in modeling both spatial and temporal nonstationarity simultaneously.
Abstract: By incorporating temporal effects into the geographically weighted regression (GWR) model, an extended GWR model, geographically and temporally weighted regression (GTWR), has been developed to deal with both spatial and temporal nonstationarity simultaneously in real estate market data. Unlike the standard GWR model, GTWR integrates both temporal and spatial information in the weighting matrices to capture spatial and temporal heterogeneity. The GTWR design embodies a local weighting scheme wherein GWR and temporally weighted regression (TWR) become special cases of GTWR. In order to test its improved performance, GTWR was compared with global ordinary least squares, TWR, and GWR in terms of goodness-of-fit and other statistical measures using a case study of residential housing sales in the city of Calgary, Canada, from 2002 to 2004. The results showed that there were substantial benefits in modeling both spatial and temporal nonstationarity simultaneously. In the test sample, the TWR, GWR, and GTWR models, respectively, reduced absolute errors by 3.5%, 31.5%, and 46.4% relative to a global ordinary least squares model. More impressively, the GTWR model demonstrated a better goodness-of-fit (0.9282) than the TWR model (0.7794) and the GWR model (0.8897). McNamara's test supported the hypothesis that the improvements made by GTWR over the TWR and GWR models are statistically significant for the sample data.
708 citations
••
TL;DR: In a recent study, it was found that Cas 12a, which belongs to the class 2 type V-A CRISPR-Cas system, performed collateral cleavage on non-targeted ssDNAs upon the formation of the Cas12a/crRNA/target DNA ternary complex.
Abstract: Dear Editor, Today, the need for time-effective and cost-effective nucleic acid detection methods is still growing in fields such as human genotyping and pathogen detection. Using synthetic biomolecular components, many methods have been developed for fast nucleic acid detection; however, they may not be able to satisfy specificity, sensitivity, speed, cost and simplicity at the same time. Recently, a very promising CRISPR-based diagnostic (CRISPR-Dx) (namely SHERLOCK) was established, which was based on the collateral effect of an RNA-guided and RNAtargeting CRISPR effector, Cas13a. SHERLOCK is of high sensitivity and specificity, and is very convenient in detection of target RNA. However, to detect DNA sequences, in vitro transcription of DNA to RNA must be conducted prior to the SHERLOCK test, which could be inconvenient. In a recent study, we found that Cas12a, which belongs to the class 2 type V-A CRISPR-Cas system, performed collateral cleavage on non-targeted ssDNAs upon the formation of the Cas12a/crRNA/target DNA ternary complex. Here, with the employment of this feature, we used a quenched fluorescent ssDNA reporter (e.g., HEX-N12-BHQ1 in Supplementary Table S1) as the probe, and developed HOLMES (an one-HOur Low-cost Multipurpose highly Efficient System), which could be used for fast detection of target DNA as well as target RNA. In HOLMES, if a target DNA exists in the reaction system, the Cas12a/crRNA binary complex forms a ternary complex with the target DNA, which will then trans-cleave non-targeted ssDNA reporter in the system, illuminating the HEX fluorescence (or any other fluorescence) (Fig. 1a). We ever purified ten Cas12a proteins (Supplementary Table S3) and found all showed the ssDNA trans-cleavage activity. To find the most suitable Cas12a for HOLMES (i.e., with high signal-to-noise ratios), we tested all ten Cas12a proteins and found Lachnospiraceae bacterium ND2006 Cas12a (LbCas12a), Oribacterium sp. NK2B42 Cas12a (OsCas12a), Lachnospiraceae bacterium NC2008 Cas12a (Lb5Cas12a) and Francisella tularensis Cas12a (FnCas12a) showed good performance, among which LbCas12a was chosen for the following studies (Fig. 1b). To determine the sensitivity of HOLMES, we titrated target DNA, and found the minimum detectable concentration for Cas12a-crRNA was approximately 0.1 nM; however, when combined with PCR, the detectable concentration could be as low as 10 aM (Fig. 1c), which was comparable to the SHERLOCK system and was better than PCR alone or quantitative PCR using the SYBR Green method (Supplementary Figure S1). Therefore, to achieve higher sensitivity, PCR amplification was employed in the HOLMES test thereafter. To test whether HOLMES could discriminate singlebase differences, we made point mutations at different positions in the target DNA sequence, including both the PAM region and the guide sequences (Supplementary Figure S2a). When a full length of crRNA guide sequence (24-nt crRNA, Supplementary Table S2) was used, we found mutations in either the PAM sequences or the region of the 1st–7th bases of the guide sequence resulted in clear decline of the fluorescence signal; however, no significant difference was observed when the mutation was within the region of the 8th–18th bases (Supplementary Figure S2b), which was highly consistent with the previous report that the 5′-end seed region in the crRNA
708 citations
Authors
Showing all 43993 results
Name | H-index | Papers | Citations |
---|---|---|---|
Michael Marmot | 193 | 1147 | 170338 |
Jing Wang | 184 | 4046 | 202769 |
Jiaguo Yu | 178 | 730 | 113300 |
Yang Yang | 171 | 2644 | 153049 |
Mark Gerstein | 168 | 751 | 149578 |
Gang Chen | 167 | 3372 | 149819 |
Jun Wang | 166 | 1093 | 141621 |
Jean Louis Vincent | 161 | 1667 | 163721 |
Wei Zheng | 151 | 1929 | 120209 |
Rui Zhang | 151 | 2625 | 107917 |
Ben Zhong Tang | 149 | 2007 | 116294 |
Kypros H. Nicolaides | 147 | 1302 | 87091 |
Thomas S. Huang | 146 | 1299 | 101564 |
Galen D. Stucky | 144 | 958 | 101796 |
Joseph J.Y. Sung | 142 | 1240 | 92035 |