Tokyo Institute of Technology

About: Tokyo Institute of Technology is a education organization based out in Tokyo, Tôkyô, Japan. It is known for research contribution in the topics: Thin film & Catalysis. The organization has 46775 authors who have published 101656 publications receiving 2357893 citations. The organization is also known as: Tokyo Tech & Tokodai.

Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor

Abstract: Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.

Quantized conductance through individual rows of suspended gold atoms

Abstract: As the scale of microelectronic engineering continues to shrink, interest has focused on the nature of electron transport through essentially one-dimensional nanometre-scale channels such as quantum wires1 and carbon nanotubes2,3. Quantum point contacts (QPCs) are structures (generally metallic) in which a ‘neck’ of atoms just a few atomic diameters wide (that is, comparable to the conduction electrons' Fermi wavelength) bridges two electrical contacts. They can be prepared by contacting a metal surface witha scanning tunnelling microscope (STM)4,5,6,7 and by other methods8,9,10,11,12, and typically display a conductance quantized in steps of 2e2/h(∼13 kΩ−1)13,14, where e is the electron charge and h is Planck's constant. Here we report conductance measurements on metal QPCs prepared with an STM that we can simultaneously image using an ultrahigh-vacuum electron microscope, which allows direct observation of the relation between electron transport and structure. We observe strands of gold atoms that are about one nanometre long and one single chain of gold atoms suspended between the electrodes. We can thus verify that the conductance of a single strand of atoms is 2e2/h and that the conductance of a double strand is twice as large, showing that equipartition holds for electron transport in these quantum systems.

Cohesin mediates transcriptional insulation by CCCTC-binding factor

Abstract: Cohesin complexes mediate sister-chromatid cohesion in dividing cells but may also contribute to gene regulation in postmitotic cells. How cohesin regulates gene expression is not known. Here we describe cohesin-binding sites in the human genome and show that most of these are associated with the CCCTC-binding factor (CTCF), a zinc-finger protein required for transcriptional insulation. CTCF is dispensable for cohesin loading onto DNA, but is needed to enrich cohesin at specific binding sites. Cohesin enables CTCF to insulate promoters from distant enhancers and controls transcription at the H19/IGF2 (insulin-like growth factor 2) locus. This role of cohesin seems to be independent of its role in cohesion. We propose that cohesin functions as a transcriptional insulator, and speculate that subtle deficiencies in this function contribute to 'cohesinopathies' such as Cornelia de Lange syndrome.