Showing papers by "Torrey Pines Institute for Molecular Studies published in 1975"
TL;DR: Results with both instruments for 150 samples correlated well with those obtained by the same enzymatic manual method and by the AutoAnalyzer fluorometric procedure.
Abstract: A procedure for enzymatic determination of serum triglycerides [Clin. Chem. 19, 476 (1973)] has been adapted for use in continuous-flow analysis (Technicon AutoAnalyzer). A very simple manifold is used; serum is incubated at 37 degrees C with the lipase and alpha-chymotrypsin in potassium phosphate buffer (0.1 mol/liter, pH 7, containing 1.50 g of bovine serum albumin per liter). The liberated glycerol is dialyzed against the complete glycerol reagent. The change in absorbance at 340 nm resulting from oxidation of NADH is proportional to the dialyzed glycerol. The same manifold can be used to determine preformed glycerol if the hydrolyzing enzymes are omitted. The hydrolysis is complete, as shown by the use of equivalent glycerol standards. No prior treatment of the samples is necessary. Assays are run at 60 per hour in the AutoAnalyzer l, 80 per hour in the AutoAnalyzer ll. Results with both instruments for 150 samples correlated well with those obtained by the same enzymatic manual method and by the AutoAnalyzer fluorometric procedure.
36 citations
TL;DR: In the procedure, reagents are used that are available commercially in the form of stable, dry powders; the method for the preparation of the reagents has been changed to achieve improved stability and performance.
Abstract: We describe a kinetic method for assay of serum or plasma triglycerides, by use of an enzymatic hydrolysis and reaction sequence already described [Clin. Chem. 19, 476 (1973)]. The reaction is triggered by addition of lipase, at a time when free glycerol, or pyruvate (or both) are no longer present. In this method, therefore, there is no need for a blank glycerol assay. In the procedure, reagents are used that are available commercially in the form of stable, dry powders; the method for the preparation of the reagents has been changed to achieve improved stability and performance. Stability and recovery of added triglycerides are satisfactory.
18 citations
Patent•
13 Jan 1975TL;DR: Low bulk density products containing at least 10% Wegscheider's Salt (Na2 CO3.3NaHCO3), by weight, in needle-like form are produced from sodium bicarbonate by heating in two distinct steps an aqueous feed mixture comprising by weight at least about 50% SBS and about 3 to 25% WSS as mentioned in this paper.
Abstract: Low bulk density products containing at least 10% Wegscheider's Salt (Na2 CO3.3NaHCO3), by weight, in needlelike form are produced from sodium bicarbonate by heating in two distinct steps an aqueous feed mixture comprising by weight at least about 50% sodium bicarbonate and about 3 to 25% Wegscheider's Salt.
11 citations
Patent•
07 Jan 1975
TL;DR: The plating bath of as mentioned in this paper is an anode-free aqueous bath comprising, per liter of bath, from about 150 to about 700 grams of bath of fluoboric acid; from 2 to about 15 grams of a plate modifying additive, preferably an amino acid chain compound; from about 15 to about 82 grams of metal selected from stannous tin or a mixture of stanous tin and lead, which mixture contains at least 5 weight percent tin, and from about 0.01 to about 1 gram of beta naphtol.
Abstract: The plating bath of the invention is an aqueous plating bath comprising, per liter of bath, from about 150 to about 700 grams of bath of fluoboric acid; from about 2 to about 15 grams of a plate modifying additive, preferably an amino acid chain compound; from about 15 to about 82 grams of metal selected from stannous tin or a mixture of stannous tin and lead, which mixture contains at least 5 weight percent tin, and from about 0.01 to about 1 gram of beta-naphtol. An electrically conductive substrate is plated with tin or a tin-lead alloy by immersing the substrate in the above plating bath at a temperature between about 50° and 100° F. and applying sufficient negative voltage to the substrate relative to an anode within the bath to cause a current density at the substrate of between about 7 and about 35 amperes per square foot.
2 citations