Institution
Toyota
Company•Safenwil, Switzerland•
About: Toyota is a company organization based out in Safenwil, Switzerland. It is known for research contribution in the topics: Internal combustion engine & Exhaust gas. The organization has 40032 authors who have published 55003 publications receiving 735317 citations. The organization is also known as: Toyota Motor Corporation & Toyota Jidosha KK.
Papers published on a yearly basis
Papers
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TL;DR: Time-lapse observations using an incubator with an integrated optical microscope may therefore be safely utilized in clinical practice, and no significant differences were observed in the fertilization rate or the rate of excellent-good cleavage embryos.
Abstract: Purpose
To assess the effects of light from an integrated optical microscope and evaluate the safety of time-lapse observations using a built-in microscope incubator.
100 citations
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TL;DR: In this article, a 2D discrete element method (DEM) was used to analyze the performance of a wheel for a lunar micro rover on sloped terrain by using a slope test bed.
100 citations
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TL;DR: The results provide a novel approach for efficient bioconversion of crystalline cellulose into glucose by use of the cellulose-disrupting protein AfSwo1, cloned from the filamentous fungus Aspergillus fumigatus.
Abstract: Cellulose is the primary polysaccharide of plant cell wall and the most abundant renewable biomass resource. Biological degradation of cellulose to soluble sugars has long been considered an alternative to the use of starch feedstocks for bioethanol production. Natural cellulose is an ordered, linear polymer of thousands of d-glucose residues linked by β-1,4-glucosidic bonds. Spontaneous crystallization of cellulose molecules due to chemical uniformity of glucose units and the high degree of hydrogen bonding in cellulose can often result in the formation of tightly packed microfibrils (8), which remain inaccessible to cellulolytic enzymes. No single enzyme is able to hydrolyze crystalline cellulose microfibrils completely. Synergistic effects of cellulase mixtures on crystalline cellulose degradation are well known (1, 7, 21). Nevertheless, cost-competitive technology for overcoming the recalcitrance of cellulosic biomass to enhance enzymatic saccharification is still a major impediment to the utilization of cellulosic materials in bioenergy generation.
Expansins are plant cell wall proteins that cause cell wall enlargement by a unique loosening effect in an acid-induced manner (15, 20). They are also involved in many physiological processes where cell wall extension occurs, such as pollination, fruit ripening, organ abscission, and seed germination (13, 14). It has been proposed that plant expansins disrupt hydrogen bonding between cellulose microfibrils and other cell wall polysaccharides without hydrolytic activity, causing sliding of cellulose fibers or expansion of the cell wall (18, 19, 27). Swollenin, an expansin-like protein, was isolated and characterized from the cellulolytic filamentous fungus Trichoderma reesei. It has a bimodular structure consisting of a carbohydrate-binding module family 1 (CBM1) domain and an expansin-like domain connected by a linker region rich in serine and threonine. Swollenin exhibits disruption activity on cellulosic materials such as cotton and algal cell walls without releasing any detectable reducing sugars (23). However, effects of cellulose disruption activity on degradation/saccharification of crystalline cellulose have not yet been reported.
Here, we report cloning a swollenin-like gene (designated Afswo1) from the filamentous fungus Aspergillus fumigatus. We also report its production by Aspergillus oryzae and characterization of the purified AfSwo1.
100 citations
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TL;DR: In this paper, the authors investigated the phenomenon of water freezing below freezing point in polymer electrolyte fuel cells (PEFCs) and found that super-cooled water was generated on the gas diffusion layer (GDL) surface and that water freezing occurs at the interface between the GDL and MEA (membrane electrode assembly) at the moment cell performance deteriorated under conditions when remaining water was dry enough inside the fuel cell before cold starting.
100 citations
Authors
Showing all 40045 results
Name | H-index | Papers | Citations |
---|---|---|---|
Derek R. Lovley | 168 | 582 | 95315 |
Edward H. Sargent | 140 | 844 | 80586 |
Shanhui Fan | 139 | 1292 | 82487 |
Susumu Kitagawa | 125 | 809 | 69594 |
John B. Buse | 117 | 521 | 101807 |
Meilin Liu | 117 | 827 | 52603 |
Zhongfan Liu | 115 | 743 | 49364 |
Wolfram Burgard | 111 | 728 | 64856 |
Douglas R. MacFarlane | 110 | 864 | 54236 |
John J. Leonard | 109 | 676 | 46651 |
Ryoji Noyori | 105 | 627 | 47578 |
Stephen J. Pearton | 104 | 1913 | 58669 |
Lajos Hanzo | 101 | 2040 | 54380 |
Masashi Kawasaki | 98 | 856 | 47863 |
Andrzej Cichocki | 97 | 952 | 41471 |