Institution
Transgene SA
Company•Illkirch-Graffenstaden, France•
About: Transgene SA is a company organization based out in Illkirch-Graffenstaden, France. It is known for research contribution in the topics: Antigen & Genetic enhancement. The organization has 377 authors who have published 296 publications receiving 12537 citations.
Topics: Antigen, Genetic enhancement, Cytotoxic T cell, Immune system, Virus
Papers published on a yearly basis
Papers
More filters
••
TL;DR: Observations suggest the feasibility of in vivo CFTR gene transfer as therapy for the pulmonary manifestations of CF.
1,143 citations
••
TL;DR: The respiratory epithelium is a potential site for somatic gene therapy for the common hereditary disorders alpha 1-antitrypsin (alpha 1AT) deficiency and cystic fibrosis by infecting epithelial cells of the cotton rat respiratory tract in vitro and in vivo.
Abstract: The respiratory epithelium is a potential site for somatic gene therapy for the common hereditary disorders alpha 1-antitrypsin (alpha 1AT) deficiency and cystic fibrosis. A replication-deficient adenoviral vector (Ad-alpha 1AT) containing an adenovirus major late promoter and a recombinant human alpha 1AT gene was used to infect epithelial cells of the cotton rat respiratory tract in vitro and in vivo. Freshly isolated tracheobronchial epithelial cells infected with Ad-alpha 1AT contained human alpha 1AT messenger RNA transcripts and synthesized and secreted human alpha 1AT. After in vivo intratracheal administration of Ad-alpha 1AT to these rats, human alpha 1AT messenger RNA was observed in the respiratory epithelium, human alpha 1AT was synthesized and secreted by lung tissue, and human alpha 1AT was detected in the epithelial lining fluid for at least 1 week.
975 citations
••
TL;DR: A novel system based on the cloning and manipulation of the full-length adenovirus genome as a stable plasmid in E. coli, by using the bacterial homologous recombination machinery is described.
Abstract: Despite recent technical improvements, the construction of recombinant adenovirus vectors remains a time-consuming procedure which requires extensive manipulations of the viral genome in both Escherichia coli and eukaryotic cells. This report describes a novel system based on the cloning and manipulation of the full-length adenovirus genome as a stable plasmid in E. coli, by using the bacterial homologous recombination machinery. The efficiency and flexibility of the method are illustrated by the cloning of the wild-type adenovirus type 5 genome, the insertion of a constitutive promoter upstream from the E3 region, the replacement of the E1 region by an exogenous expression cassette, and the deletion of the E1 region. All recombinant viral DNAS were shown to be fully infectious in permissive cells, and the modified E3 region or the inserted foreign gene was correctly expressed in the infected cells.
726 citations
••
TL;DR: The ΔF508 mutation seems to have two major consequences, an abnormal translocation of the CFTR protein which limits membrane insertion, and an abnormal function in mediating Cl- transport.
Abstract: Cystic fibrosis is associated with a defect in epithelial chloride ion transport which is caused by mutations in a membrane protein called CFTR (cystic fibrosis transmembrane conductance regulator). Heterologous expression of CFTR produces cyclicAMP-sensitive Cl(-)-channel activity. Deletion of phenylalanine at amino-acid position 508 in CFTR (delta F508 CFTR) is the most common mutation in cystic fibrosis. It has been proposed that this mutation prevents glycoprotein maturation and its transport to its normal cellular location. We have expressed both CFTR and delta F508 CFTR in Vero cells using recombinant vaccinia virus. Although far less delta F508 CFTR reached the plasma membrane than normal CFTR, sufficient delta F508 CFTR was expressed at the plasma membrane to permit functional analysis. delta F508 CFTR expression induced a reduced activity of the cAMP-activated Cl- channel, with conductance, anion selectivity and open-time kinetics similar to those of CFTR, but with much greater closed times, resulting in a large decrease of open probability. The delta F508 mutation thus seems to have two major consequences, an abnormal translocation of the CFTR protein which limits membrane insertion, and an abnormal function in mediating Cl- transport.
662 citations
••
TL;DR: The observations that the highly purified chitosan fractions used were neither toxic nor haemolytic, that they have the ability to complex DNA and protect against nuclease degradation and that low molecular weight chitOSan can be administered intravenously without liver accumulation suggest there is potential to investigate further low molecular Weight chITosans as components of a synthetic gene delivery system.
530 citations
Authors
Showing all 378 results
Name | H-index | Papers | Citations |
---|---|---|---|
Xuetao Cao | 95 | 509 | 35318 |
Luc Montagnier | 76 | 410 | 30740 |
Simon Wain-Hobson | 62 | 235 | 16013 |
Catherine Tomasetto | 57 | 139 | 10479 |
Rainer Bischoff | 54 | 327 | 10146 |
Richard Lathe | 52 | 172 | 19962 |
Margaret A. Liu | 47 | 97 | 10503 |
Georges Lacaud | 45 | 102 | 5905 |
Jean-Yves Bonnefoy | 44 | 93 | 6368 |
Klaus Schughart | 40 | 157 | 6438 |
Michael Antoniou | 40 | 129 | 5158 |
Andrea Pavirani | 39 | 104 | 8283 |
A Capron | 39 | 129 | 4826 |
Jean-Pierre Lecocq | 37 | 125 | 7122 |
Stéphane Paul | 36 | 199 | 4592 |