Showing papers by "Tunis University published in 1996"
TL;DR: This methodology will allow the use of reverse genetics in the study of PVY-plant interactions and will also be useful for obtaining infectious cDNA from other viruses with large RNA genomes.
Abstract: Potato virus Y (PVY) full-length cDNA has been found to be refractory to cloning in Escherichia coli cells. A full-length 9.7 kb PVY cDNA was obtained by reverse transcription polymerase chain reaction (RT-PCR) from the RNA of PVY (tuber necrotic strain, PVYNTN). Double-stranded DNA fragments were used as primers (ds megaprimers), to include signals for transcription in vivo (a cauliflower mosaic virus 35S RNA promoter and a nopaline synthase terminator) in the final PCR product. Biolistic bombardment with a helium particle gun was used to inoculate the amplified product to detached tobacco leaves. Inoculation of tobacco plants with ground inoculated leaves followed by northern blot, ELISA and immuno-electron microscopy demonstrated that the DNA was highly infectious with up to 90% of bombarded leaves containing the virus. This methodology will allow the use of reverse genetics in the study of PVY-plant interactions and will also be useful for obtaining infectious cDNA from other viruses with large RNA genomes.
63 citations
TL;DR: Campbell, J. Campbell, V. Halliday, M. Carroll, and S. Hood as discussed by the authors developed integrated approaches to assessment and explored course design in a changing curriculum to explore learners' views.
Abstract: Campbell, J. (1985). Towards active voice: Report of the Committee of Review of the Adult Migrant Education Program. Canberra: Australian Commonwealth Government Publishing Service. Campbell, V. (1995). Competency-based vocational language learning: Exploring learners' views. In A. Burns & S. Hood (Eds.), Teachers'voices: Exploring course design in a changing curriculum (pp. 123-130). Sydney, Australia: NCELTR. Carroll, M. (1995). Developing integrated approaches to assessment. In A. Burns & S. Hood (Eds.), Teachers' voices: Exploring course design in a changing curriculum (pp. 95-105). Sydney, Australia: NCELTR. Colman, J. (1991). Towards a coherent curriculum frame: Learner pathways in the NSW Adult Migrant English Service. Prospect, 7(1), 28-42. Halliday, M. A. K. (1985). An introduction to functional grammar. London: Edward Arnold.
26 citations
TL;DR: In this article, a model of turbulence with second-order closure is used to study the properties of turbulent jets with major variations in density, and a Favre average procedure is employed to validate the performance of this model.
20 citations
TL;DR: In this article, the thermal behavior of the Casilina bridge over a motorway near Rome, Italy, was analyzed based on a three-year monitoring program. But the authors focused on a finite element model for trans...
Abstract: This paper analyzes the thermal behaviour of the prestressed, box girder Casilina Bridge over a motorway near Rome, Italy, based on a three-year monitoring program. A finite element model for trans...
15 citations
01 Jan 1996
TL;DR: In this paper, the authors define the class of H*V-A-modules, which are modulo 2 equivariante equivariant modulo equivariants of a V-space.
Abstract: Soient V un 2-groupe abelien elementaire (en d’autres termes, un \({\Bbb F}\) 2-espace vectoriel de dimension finie) et A l’algebre de Steenrod modulo 2; H*V designe la cohomologie modulo 2 de V. Un H*V-A-module instable est un A-module instable M muni d’une structure de H*V-module definie par une application H*V ⊗ M → M qui est A-lineaire (l’exemple type d’un tel objet est la cohomologie modulo 2 equivariante d’un V-espace). Cet article poursuit l’etude, commencee dans [DW] [LZ], de la categorie des H*V-A-modules instables qui sont de type fini comme H*V-modules.
4 citations
11 Feb 1996-Nuclear Instruments & Methods in Physics Research Section A-accelerators Spectrometers Detectors and Associated Equipment
TL;DR: In this article, it was shown that in a non-cubic crystal, the extra heat capacity due to quadrupolar interaction of nuclear spins > 1 2 could be much bigger than the phonon heat capacity when the temperature decreases.
Abstract: It is shown that in a non-cubic crystal, the extra heat capacity due to quadrupolar interaction of nuclear spins > 1 2 could be much bigger than the phonon heat capacity when the temperature decreases. The possible coupling between quadrupolar and phonon heat reservoir via paramagnetic impurities is stressed. A NMR experiment done on sapphire is presented with an evaluation of the coupling between the two reservoirs and its consequence on the performance of the bolometer.
4 citations
3 citations
TL;DR: In this paper, the authors investigated the solubility properties of the phosphogypsum sideproduct of the Phosphor industry and presented phase diagrams of potassium, calcium, hydrogen phosphates, sulphates and water.
Abstract: The aim of the study was to investigate the solubility properties of the phosphogypsum a sideproduct of phosphor industry. Phase diagrams of potassium, calcium, hydrogen phosphates, sulphates and water are presented in order to determine equilibrium of solubility. Solid phases were investigated by X-ray diffraction, IR spectrometry and thermal analysis (DTG-DTA).
1 citations
TL;DR: Soit (Vλ)λ≥0 une resolvante definie sur un espace mesurable telle que le noyau initial est borne; on trouve une condition necessaire et suffisante pour qu'un noyaul borne U possede une resOLvante (Uλ,λ ≥ 0 telle that Uλ≦Vλ pour tout λ ≥ 0. On donne plusieurs applications de ce resultat
Abstract: Soit (Vλ)λ≥0 une resolvante definie sur un espace mesurable telle que le noyau initial est borne; on trouve une condition necessaire et suffisante pour qu'un noyau borne U possede une resolvante (Uλ)λ≥0 telle que Uλ≥Vλ pour tout λ≥0. On donne plusieurs applications de ce resultat.
TL;DR: In this paper, three-dimensional potential energy functions for the lowest X 2 B 1, A 2 A 1 and 2 B 2 states of the H2Se+ radical cation have been generated by ab initio calculations and used in the evaluation of the spectroscopic constants of the X2B1 and A2A1 states.
Abstract: Three-dimensional potential energy functions for the lowest X 2 B 1 , A 2 A 1 and 2 B 2 states of the H2Se+ radical cation have been generated by ab initio calculations and used in the evaluation of the spectroscopic constants of the X2B1 and A2A1 states. Variationally calculated vibrational levels up to 10 000 cm−1 of the X state and the low lying vibrational levels of the A state are reported. The largest absolute line intensities obtained from theoretical dipole moment functions and rovibrational wave functions are given for the fundamental vibrational transitions.