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Institution

Umeå University

EducationUmeå, Sweden
About: Umeå University is a education organization based out in Umeå, Sweden. It is known for research contribution in the topics: Population & Poison control. The organization has 18362 authors who have published 53564 publications receiving 2250438 citations. The organization is also known as: Umeå universitet & umu.se.


Papers
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Journal ArticleDOI
TL;DR: An overview of important concepts related to qualitative content analysis is provided and measures to achieve trustworthiness (credibility, dependability and transferability) throughout the steps of the research procedure are proposed.

16,695 citations

Journal ArticleDOI
17 Aug 2012-Science
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. We show here that in a subset of these systems, the mature crRNA that is base-paired to trans-activating crRNA (tracrRNA) forms a two-RNA structure that directs the CRISPR-associated protein Cas9 to introduce double-stranded (ds) breaks in target DNA. At sites complementary to the crRNA-guide sequence, the Cas9 HNH nuclease domain cleaves the complementary strand, whereas the Cas9 RuvC-like domain cleaves the noncomplementary strand. The dual-tracrRNA:crRNA, when engineered as a single RNA chimera, also directs sequence-specific Cas9 dsDNA cleavage. Our study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.

12,865 citations

Journal ArticleDOI
TL;DR: The Global Burden of Diseases, Injuries, and Risk Factors Study 2016 (GBD 2016) provides a comprehensive assessment of prevalence, incidence, and years lived with disability (YLDs) for 328 causes in 195 countries and territories from 1990 to 2016.

10,401 citations

Journal ArticleDOI
TL;DR: The autoxidation of pyrogallol was investigated in the presence of EDTA in the pH range 7.9–10.6, indicating an almost total dependence on the participation of the superoxide anion radical, O2·−, in the reaction.
Abstract: The autoxidation of pyrogallol was investigated in the presence of EDTA in the pH range 7.9–10.6. The rate of autoxidation increases with increasing pH. At pH 7.9 the reaction is inhibited to 99% by superoxide dismutase, indicating an almost total dependence on the participation of the superoxide anion radical, O2·−, in the reaction. Up to pH 9.1 the reaction is still inhibited to over 90% by superoxide dismutase, but at higher alkalinity, O2·− -independent mechanisms rapidly become dominant. Catalase has no effect on the autoxidation but decreases the oxygen consumption by half, showing that H2O2 is the stable product of oxygen and that H2O2 is not involved in the autoxidation mechanism. A simple and rapid method for the assay of superoxide dismutase is described, based on the ability of the enzyme to inhibit the autoxidation of pyrogallol. A plausible explanation is given for the non-competitive part of the inhibition of catechol O-methyltransferase brought about by pyrogallol.

9,030 citations

Journal ArticleDOI
TL;DR: Principal Component Analysis is a multivariate exploratory analysis method useful to separate systematic variation from noise and to define a space of reduced dimensions that preserve noise.

8,660 citations


Authors

Showing all 18544 results

NameH-indexPapersCitations
John Hardy1771178171694
Erkki Ruoslahti159600101072
Elio Riboli1581136110499
Johan G. Eriksson1561257123325
Bengt Winblad1531240101064
Stephen T. Holgate14287082345
Anne Tjønneland139134591556
Martin McKee1381732125972
Paolo Vineis134108886608
Hugh Watkins12852491317
Paul Zimmet128740140376
Göran Hallmans12874378391
Desire Collen12467967126
Jun Yu121117481186
Alan R. Shuldiner12055771737
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
202393
2022254
20212,814
20202,623
20192,473
20182,311