scispace - formally typeset
Search or ask a question
Institution

Universidade Estadual de Londrina

EducationLondrina, Brazil
About: Universidade Estadual de Londrina is a education organization based out in Londrina, Brazil. It is known for research contribution in the topics: Population & Toxoplasma gondii. The organization has 13052 authors who have published 19291 publications receiving 212123 citations.


Papers
More filters
Journal ArticleDOI
17 May 2018-Cell
TL;DR: It is concluded that targeting the peripheral nervous system and blocking neuro-immune communication is a promising strategy to treat highly invasive bacterial infections.

236 citations

Journal ArticleDOI
TL;DR: The exposure to RDT for 96 h led to an inhibition of AChE in brain and muscle at rates which may not be considered a life-threatening situation, and after 24 and 96 h the antioxidant defenses were apparently enough to combat ROS, preventing the occurrence of oxidative damage.

233 citations

Journal ArticleDOI
01 Feb 1998
TL;DR: Aprendizagem baseada em Problemas, enquanto a metodologia da problematizacao de ensino, are two important propostas inovadoras, which se desenvolvem a partir de visoes teoricas distintas, tem pontos comuns e pontos diferentes as discussed by the authors.
Abstract: Duas importantes propostas inovadoras sao objeto de descricao e analise comparativa: a Metodologia da Problematizacao e a Aprendizagem Baseada em Problemas. As duas propostas, que se desenvolvem a partir de visoes teoricas distintas, tem pontos comuns e pontos diferentes. Nas duas propostas, o ensino e a aprendizagem ocorrem a partir de problemas. Na Metodologia da Problematizacao, enquanto alternativa de metodologia de ensino, os problemas sao extraidos da realidade pela observacao realizada pelos alunos. Na Aprendizagem Baseada em Problemas, enquanto proposta curricular, os problemas de ensino sao elaborados por uma equipe de especialistas para cobrir todos os conhecimentos essenciais do curriculo. O conhecimento de suas caracteristicas nao permite confundi-las, mas, com certeza, toma-las como alternativas inspiradoras de um ensino inovador que ultrapasse a abordagem tradicional.

230 citations

Journal ArticleDOI
TL;DR: In this paper, a new morphological, genotypic and genomic evidence was presented to support reclassification into a novel species, for which the name "Bradyrhizobium petertodddiazoefficiens sp. nov" was proposed.
Abstract: Bradyrhizobium japonicum was described from soybean root-nodule bacterial isolates. Since its description, several studies have revealed heterogeneities among rhizobia assigned to this species. Strains assigned to B. japonicum group Ia have been isolated in several countries, and many of them are outstanding soybean symbionts used in inoculants worldwide, but they have also been isolated from other legume hosts. Here, we summarize published studies that indicate that group Ia strains are different from the B. japonicum type strain USDA 6T and closely related strains, and present new morphophysiological, genotypic and genomic evidence to support their reclassification into a novel species, for which the name Bradyrhizobium diazoefficiens sp. nov. is proposed. The type strain of the novel species is the well-studied strain USDA 110T ( = IAM 13628T = CCRC 13528T = NRRL B-4361T = NRRL B-4450T = TAL 102T = BCRC 13528T = JCM 10833T = TISTR 339T = SEMIA 5032T = 3I1B110T = ACCC 15034T = CCT 4249T = NBRC 14792T = R-12974T = CNPSo 46T).

229 citations

Journal ArticleDOI
23 May 2014-PLOS ONE
TL;DR: Results show that metformin has an antiproliferative effect associated with cell cycle arrest and apoptosis, which is mediated by oxidative stress, as well as AMPK and FOXO3a activation.
Abstract: Recent studies have demonstrated that the anti-diabetic drug, metformin, can exhibit direct antitumoral effects, or can indirectly decrease tumor proliferation by improving insulin sensitivity. Despite these recent advances, the underlying molecular mechanisms involved in decreasing tumor formation are not well understood. In this study, we examined the antiproliferative role and mechanism of action of metformin in MCF-7 cancer cells treated with 10 mM of metformin for 24, 48, and 72 hours. Using BrdU and the MTT assay, it was found that metformin demonstrated an antiproliferative effect in MCF-7 cells that occurred in a time- and concentration- dependent manner. Flow cytometry was used to analyze markers of cell cycle, apoptosis, necrosis and oxidative stress. Exposure to metformin induced cell cycle arrest in G0-G1 phase and increased cell apoptosis and necrosis, which were associated with increased oxidative stress. Gene and protein expression were determined in MCF-7 cells by real time RT-PCR and western blotting, respectively. In MCF-7 cells metformin decreased the activation of IRβ, Akt and ERK1/2, increased p-AMPK, FOXO3a, p27, Bax and cleaved caspase-3, and decreased phosphorylation of p70S6K and Bcl-2 protein expression. Co-treatment with metformin and H2O2 increased oxidative stress which was associated with reduced cell number. In the presence of metformin, treating with SOD and catalase improved cell viability. Treatment with metformin resulted in an increase in p-p38 MAPK, catalase, MnSOD and Cu/Zn SOD protein expression. These results show that metformin has an antiproliferative effect associated with cell cycle arrest and apoptosis, which is mediated by oxidative stress, as well as AMPK and FOXO3a activation. Our study further reinforces the potential benefit of metformin in cancer treatment and provides novel mechanistic insight into its antiproliferative role.

227 citations


Authors

Showing all 13138 results

NameH-indexPapersCitations
Michael Maes11580752050
Fernando Q. Cunha8868231501
Mariangela Hungria6738915219
Petar Popovski5975621009
Waldiceu A. Verri5424910311
Thiago M. Cunha542689519
Emerson Franchini524029620
Celso Vataru Nakamura5141810908
Diego Augusto Santos Silva5138953077
Susan M. Tarlo5026310850
Paulo Caramelli453669666
Fabio Pitta4421311925
Joaquim Gama-Rodrigues432258380
Ricardo Almeida432507304
Hamilton Roschel432355894
Network Information
Related Institutions (5)
Sao Paulo State University
100.4K papers, 1.3M citations

93% related

Federal University of Paraná
46.6K papers, 546.5K citations

92% related

Universidade Federal do Rio Grande do Sul
89.4K papers, 1.4M citations

91% related

Universidade Federal de Minas Gerais
75.6K papers, 1.2M citations

90% related

Universidade Federal de Santa Catarina
55.4K papers, 714.4K citations

90% related

Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
202324
2022151
20211,220
20201,433
20191,333
20181,308