Institution
Universidade Estadual de Londrina
Education•Londrina, Brazil•
About: Universidade Estadual de Londrina is a education organization based out in Londrina, Brazil. It is known for research contribution in the topics: Population & Toxoplasma gondii. The organization has 13052 authors who have published 19291 publications receiving 212123 citations.
Topics: Population, Toxoplasma gondii, Oxidative stress, Starch, Germination
Papers published on a yearly basis
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TL;DR: The goal was to identify genes expressed in the mycelium and yeast forms by EST sequencing in order to generate a partial map of the fungus transcriptome and identify some genes potentially involved in the dimorphic transition and drug resistance.
Abstract: Paracoccidioides brasiliensis is a pathogenic fungus that undergoes a temperature-dependent cell morphology change from mycelium (22 degrees C) to yeast (36 degrees C). It is assumed that this morphological transition correlates with the infection of the human host. Our goal was to identify genes expressed in the mycelium (M) and yeast (Y) forms by EST sequencing in order to generate a partial map of the fungus transcriptome. Individual EST sequences were clustered by the CAP3 program and annotated using Blastx similarity analysis and InterPro Scan. Three different databases, GenBank nr, COG (clusters of orthologous groups) and GO (gene ontology) were used for annotation. A total of 3,938 (Y = 1,654 and M = 2,274) ESTs were sequenced and clustered into 597 contigs and 1,563 singlets, making up a total of 2,160 genes, which possibly represent one-quarter of the complete gene repertoire in P. brasiliensis. From this total, 1,040 were successfully annotated and 894 could be classified in 18 functional COG categories as follows: cellular metabolism (44%); information storage and processing (25%); cellular processes-cell division, posttranslational modifications, among others (19%); and genes of unknown functions (12%). Computer analysis enabled us to identify some genes potentially involved in the dimorphic transition and drug resistance. Furthermore, computer subtraction analysis revealed several genes possibly expressed in stage-specific forms of P. brasiliensis. Further analysis of these genes may provide new insights into the pathology and differentiation of P. brasiliensis.
103 citations
TL;DR: O&NS as well as lowered antioxidant levels may play a role in the pathophysiology of suicidal behavior independently from the effects of depression and smoking, both of which are associated with increased O&NS, and classical suicide predictors, such as years of education and marital status.
103 citations
TL;DR: In this article, the apparent diffusion coefficients for sucrose, NaCl and water during osmotic dehydration of tomatoes in ternary solutions were determined, and simple regression models as functions of solutes concentration were determined for diffusion coefficients.
102 citations
TL;DR: The VO2max plays a key role in determining the magnitude of an individual's perceived exertion during futsal training sessions, as measured by the session rating of perceived exertedion (session-RPE) method.
Abstract: Purpose: The aim of this study was to verify the influence of aerobic fitness (VO2max) on internal training loads, as measured by the session rating of perceived exertion (session-RPE) method. Methods: Nine male professional outfield futsal players were monitored for 4 wk of the in-season period with regards to the weekly accumulated session-RPE, while participating in the same training sessions. Single-session-RPE was obtained from the product of a 10-point RPE scale and the duration of exercise. Maximal oxygen consumption was determined during an incremental treadmill test. Results: The average training load throughout the 4 wk period varied between 2,876 and 5,035 arbitrary units. Technical-tactical sessions were the predominant source of loading. There was a significant cor relation between VO2max (59.6 ± 2.5 mL ∙ kg–1 ∙ min–1) and overall training load accumulated over the total period (r = –0.75). Conclusions: The VO2max plays a key role in determining the magnitude of an individual’s perceived exertion during futsal training sessions.
102 citations
University of California, Irvine1, Massachusetts Institute of Technology2, Sao Paulo State University3, Universidade Federal de Santa Maria4, Universidade Federal do Rio Grande do Sul5, Universidade Federal de Minas Gerais6, University of Massachusetts Medical School7, Oswaldo Cruz Foundation8, Universidade Federal de Santa Catarina9, Federal University of Piauí10, State University of Ceará11, University of Connecticut Health Center12, J. Craig Venter Institute13, State University of Campinas14, Universidade Federal de Viçosa15, Federal University of Amazonas16, Universidade Federal de Goiás17, Federal University of Paraíba18, Universidade Estadual de Londrina19, Federal University of Rio de Janeiro20, National Institute of Amazonian Research21, University of São Paulo22, National Institutes of Health23, Federal University of Rio Grande do Norte24, University of Brasília25, Universidade Federal Rural de Pernambuco26, Universidade Católica de Brasília27, Empresa Brasileira de Pesquisa Agropecuária28
TL;DR: The first effort to sequence the genome of a neotropical malaria vector, and open a new window through which we can contemplate the evolutionary history of anopheline mosquitoes, is presented in this article, which provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent.
Abstract: Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vector-human and vector-parasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles-darlingi.
102 citations
Authors
Showing all 13138 results
| Name | H-index | Papers | Citations |
|---|---|---|---|
| Michael Maes | 115 | 807 | 52050 |
| Fernando Q. Cunha | 88 | 682 | 31501 |
| Mariangela Hungria | 67 | 389 | 15219 |
| Petar Popovski | 59 | 756 | 21009 |
| Waldiceu A. Verri | 54 | 249 | 10311 |
| Thiago M. Cunha | 54 | 268 | 9519 |
| Emerson Franchini | 52 | 402 | 9620 |
| Celso Vataru Nakamura | 51 | 418 | 10908 |
| Diego Augusto Santos Silva | 51 | 389 | 53077 |
| Susan M. Tarlo | 50 | 263 | 10850 |
| Paulo Caramelli | 45 | 366 | 9666 |
| Fabio Pitta | 44 | 213 | 11925 |
| Joaquim Gama-Rodrigues | 43 | 225 | 8380 |
| Ricardo Almeida | 43 | 250 | 7304 |
| Hamilton Roschel | 43 | 235 | 5894 |