Universidade Estadual de Londrina

About: Universidade Estadual de Londrina is a education organization based out in Londrina, Brazil. It is known for research contribution in the topics: Population & Context (language use). The organization has 13052 authors who have published 19291 publications receiving 212123 citations.

Caminhos para aproximar a formacao de profissionais de saude das necessidades da atencao basica

Abstract: Resumo: A partir da consideracao de que o Sistema Unico de Saude, por mandato constitucional, deveria “ordenar” o processo de formacao profissional na area da saude e com base na importância da forca de trabalho que ele absorve, os autores exploram as possibilidades de integracao do processo de aprendizagem a rede de servicos de saude. Na busca de reorientar a formacao, tratam de estimular uma atuacao interdisciplinar multiprofissional por meio da construcao de um novo modelo pedagogico que equilibre a excelencia tecnica e a relevância social, com metodos de ensino-aprendizagem centrados no aluno e desenvolvidos como processo permanente, com base nas relacoes de parceria da universidade com os servicos de saude, com a comunidade, com as entidades e outros setores da sociedade civil. Os autores propoem um sistema de incentivos as instituicoes de ensino superior, visando maior sintonia com o paradigma da integralidade. Para isto, estabelecem um mecanismo de acompanhamento classificatorio que congrega os distintos eixos de um processo de mudanca da organizacao de uma instituicao de ensino na direcao proposta, que incluem: a orientacao teorica predominante na instituicao, a abordagem pedagogica e os cenarios de praticas, cada um deles abrindo-se em tres vetores que marcam o grau de avanco nas mudancas em curso. Com isso, conformam uma tipologia em um “perfil radical de avaliacao” da tendencia a alcancar o paradigma proposto. Nao se avalia com este instrumento a qualidade da instituicao ou do programa, senao sua posicao em relacao ao SUS.

Effect of elatol, isolated from red seaweed Laurencia dendroidea, on Leishmania amazonensis.

Abstract: In the present study, we investigated the antileishmanial activity of sesquiterpene elatol, the major constituent of the Brazilian red seaweed Laurencia dendroidea (Hudson) J.V. Lamouroux, against L. amazonensis. Elatol after 72 h of treatment, showed an IC50 of 4.0 µM and 0.45 µM for promastigote and intracellular amastigote forms of L. amazonensis, respectively. By scanning and transmission electron microscopy, parasites treated with elatol revealed notable changes compared with control cells, including: pronounced swelling of the mitochondrion; appearance of concentric membrane structures inside the organelle; destabilization of the plasma membrane; and formation of membrane structures, apparently an extension of the endoplasmic reticulum, which is suggestive of an autophagic process. A cytotoxicity assay showed that the action of the isolated compound is more specific for protozoa, and it is not toxic to macrophages. Our studies indicated that elatol is a potent antiproliferative agent against promastigote and intracellular amastigote forms, and may have important advantages for the development of new anti-leishamanial chemotherapies.

Ischaemia and reperfusion effects on skeletal muscle tissue: morphological and histochemical studies.

Abstract: This was a study on the oxidative stress due to ischaemia (I) and reperfusion (R) in skeletal muscle tissue. Using a tourniquet, groups of rats were submitted to ischaemia for 4 h, followed by different reperfusion periods. The animals were divided in four groups: control; 4 h of ischaemia (IR); 4 h of ischaemia plus 1 h reperfusion (IR-1 h); 4 h of ischaemia plus 24 h reperfusion (IR-24 h); and 4 h of ischaemia plus 72 h reperfusion (IR-72 h). At the end of the procedures, samples of soleus muscle were collected and frozen in n-hexane at -70 degrees C. Cryostat sections were submitted to haematoxylin-eosin, succinate dehydrogenase (SDH) and nicotinamide adenine dinucleotide-tetrazolium reductase (NADH-TR) stains. An additional muscle sample was processed for electron microscopy. No alterations were found in control animals. IR group showed fibres had normal aspect besides some round, acidophilic and hypertrophic fibres. There were several fibres with angular outlines and smaller diameters in this group compared with control group. NADH-TR/SDH reaction was moderately intense in most fibres. In some fibres, cytoplasm showed areas without activity and other fibres had very intense reactivity. IR-1 h group showed oedema hypercontracted fibres with disorganized myofibrils, mitochondria with focal lesions and dilated sarcoplasmic reticulum. NADH-TR/SDH reaction was moderate to weak. IR-24 h showed intense inflammatory infiltrate in the endomysium and perimysium. NADH-TR/SDH reaction was similar to IR-1 h. IR-72 h showed necrotic fibres, areas with inflammatory infiltrate, reduced muscle fibres at different stages of necrosis and phagocytosis, and many small round and basophilic fibres characterizing a regeneration process. NADH-TR/SDH reaction was weak to negative. Our results suggest that ischaemia and the subsequent 1-, 24- and 72-h reperfusions induced progressive histological damage. Although progressive, it may be reversible because there were ultrastructural signs of recovery after 72-h reperfusion. This recovery could in part be due to the low oxidative stress identified by the morphological and histochemical analysis.

Uso de ferramentas por Cebus Apella (Linnaeus) (Primates, Cebidae) para obtenção de larvas de coleoptera que parasitam sementes de syagrus romanzoffianum (Cham.) Glassm. (Arecaceae)

Abstract: This paper reports the behavior of the black capped capuchin monkey, Cebus apella, using tools to break seeds of Syagrus romanzoffianum in search of coleoptera larvae that live inside. The study area was the Parque Municipal Arthur Thomas (85,47 ha) located in the city of Londrina, Parana state. In one year of study, using the ad libitum method, the monkeys were observed using rocks pretty much in the same way as if they were hammer and anvil. Individuais of both sexes including adults, subadults and juveniles with more than two years of age, were involved in such activity. The behavior of the animais consisted of they place the seeds on a large rock in the ground, and using a smaller rock which was hold among the hands, the seeds were beaten repeatedly until breaking. Then they ate the larvae and/or endosperm. The use of tools is thought to have brought benefits to the animais by allowing the access to a source of food that would not normally be used.

Analysis of football game-related statistics using multivariate techniques.

Abstract: The purpose of this study was to explore football game-related statistics during a competition, using principal component and cluster analyses to determine if it is possible to distinguish the winning teams from the drawing and losing ones. We collected the game-related statistics of the group phase matches of the 2006 World Cup and organised them into a matrix. The principal components of the covariance matrix were calculated. The scores of the first and second components were used to represent the new data, and cluster analysis was applied to separate the elements in two groups (G1 and G2). To analyse the degree of separation between the groups, we calculated the Silhouette Coefficient for each group. Finally, we checked if the winning teams were classified into the same group. The Silhouette Coefficients found for G1 and G2 were 0.54 and 0.55, respectively. Results showed that 70.3% of the winning teams were classified into the same group (G1). Similarly, 67.8% of the drawing and losing teams w...