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Showing papers by "Universidade Estadual do Vale do Acaraú published in 2002"

Journal Article•DOI•
Cowpea Vicilins: Fractionation of Urea Denatured Sub-Units and Effects on Callosobruchus maculatus F. (Coleoptera: Bruchidae) Development

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Antônio Chagas Mota1, Kátia Valevski Sales Fernandes2, Maurício P. Sales3, Victor Martin Quintana Flores2, José Xavier-Filho2 •
Universidade Estadual do Vale do Acaraú1, State University of Norte Fluminense2, Federal University of Rio Grande do Norte3
01 Mar 2002-Brazilian Archives of Biology and Technology
TL;DR: Results indicated that vicilin polypeptides of toxic nature were expressed in both types of storage globulins although at different levels.
Abstract: Vicilins (7S storage globulins) isolated from cowpea (Vigna unguiculata L.) seeds which were susceptible (S) and resistant (R) to the cowpea weevil (Callosobruchus maculatus F., Coleoptera: Bruchidae) were denatured by urea and fractionated by ion-exchange chromatography. Isolated fractions were incorporated in artificial seeds for assessment of their toxicity to C. maculatus. The most acidic fractions of both susceptible (CE-31 cultivar) and resistant (IT81D-1045 line) seeds were shown to affect development and survival of the bruchid. Results indicated that vicilin polypeptides of toxic nature were expressed in both types of storage globulins although at different levels.

15 citations