Showing papers by "Universidade Estadual do Vale do Acaraú published in 2007"

Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples

Abstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 10(3) c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.

Gravidez na adolescência: motivos e percepções de adolescentes

Abstract: Tratou-se de pesquisa exploratorio-descritiva, realizada de outubro a dezembro de 2002, com 216 adolescentes gravidas assistidas pela Estrategia de Saude da Familia dos municipios pertencentes a Microrregiao de Saude de Acarau, Ceara. Objetivou caracterizar o perfil socio-demografico e gineco-obstetrico destas, identificando o motivo que as levou a engravidar, e saber como percebiam sua gravidez. A maior parte destas tinha 17 anos, eram pardas, alfabetizadas, donas-de-casa, tinham companheiro fixo e viviam com menos de um salario minimo. Destas, 32,4% menstruou aos 12 anos, 25% teve a primeira relacao sexual aos 16 anos e, 72,7% estava na primeira gestacao. Destaca-se o desejo de ser mae como principal motivo para engravidar, e a percepcao em relacao a gravidez esta relacionada com felicidade e realizacao pessoal.

Gerentes do território na Estratégia Saúde da Família: análise e perfil de necessidades de qualificação

Abstract: O estudo objetivou elaborar o perfil socio-demografico e educacional dos gerentes de territorio da ESF e identificar os tipos de qualificacao ou processo de educacao permanente que tem ou estao inseridos os gerentes, o desenvolvimento cientifico-cultural dos mesmos. A pesquisa e do tipo exploratorio-descritiva, realizada em Sobral - Ceara, com 28 gerentes de territorio da Estrategia Saude da Familia no periodo de novembro de 2006 a junho de 2007. Para coleta de dados foi utilizado um questionario e uma entrevista semi-estruturada. O perfil socio-demografico dos gerentes mostra que: 57,2% estao na faixa etaria de 25 a 34 anos; 92,9% sao do sexo feminino; 78,6% sao casados; 57,2% tem renda mensal na ESF de R$ 1.800,00 a 2.000,00; 100% sao enfermeiros; 71,4% possuem Especializacao na modalidade de Residencia Multiprofissional em Saude da Familia e 57,1% apresentam necessidade de qualificacao em gestao em saude e gestao de pessoas. Sugere-se que a Secretaria Municipal da Saude e Acao Social podera estabelecer as atribuicoes dos gerentes e avaliar o perfil dos gerentes durante a nomeacao, e confronta-lo com o perfil que o sistema exige.

Olhares dos enfermeiros acerca de seu processo de trabalho na prescrição medicamentos na Estratégia Saúde da Família

Abstract: One of the advances in the Family Health Strategy (FHS) is the prescription of medication by nurses, which has brought the discussion together with the medical category. This study aimed at analysing nursse’s perception about this act as well as its regulation. The study is of qualitative approach, having as subjects four Nurses who perform in FHS in Sobral, Ceara. A questionnaire and the technique of focus group were used to collect data. The totality of the sample works in the rural zone and that only two had specialization. Nurses reported they are unsatisfied and unsecure related to the act of prescribing medication since the municipal protocols did not correspond to local reality. A review of the current nursing protocol for the municipality is suggested, making it suitable to complaints in the community, but without abandoning the priority of the preventive and community model that nursing performs. Descriptors: Nursing; Family Health Program; Drug Prescription; Work Process. RESUMEN

Poedeiras alimentadas com diferentes níveis de energia e oleo de soja na ração

Abstract: EnglishThe aim of this work was to evaluate increasing levels of energy and soybean oil on performance and internal and external egg quality. Three hundred and sixty semi-heavy laying hens Bovans Goldline, 29 week-old, were used, during five periods of 28 days each. It was used a completely randomized design, in a double factorial (3 x 3), totaling nine treatments, with five replicates of eight birds each. Studied factors were soybean oil (1, 2 and 3%) and energy (2600, 2750 and 2900 kcal EMkg-1) levels on diet. No interaction was observed among soybean oil and metabolizable energy. Soybean oil did not promote effect over none of the evaluated parameters. No effect was observed for energy levels on egg production, feed conversion, egg mass, yolk and shell weight, yolk and albumen percentage and specific gravity. Differences were observed for feed conversion, egg and albumen weight and shell percentage, given that the highest weights were observed at the lowest energy level and the highest percentage was observed at the highest energy level. Providing 2600 kcal EMkg-1 was more suitable in order to keep production and body weight and also to promote improvement on energetic conversion and an increase on egg weight. portuguesObjetivou-se avaliar os efeitos de niveis crescentes de energia e de oleo de soja sobre o desempenho e a qualidade interna e externa dos ovos de poedeiras semipesadas. Foram utilizadas 360 poedeiras comerciais da linhagem Bovans Goldline, com 29 semanas de idade, durante cinco periodos de 28 dias, distribuidas num delineamento inteiramente casualizado, em esquema fatorial 3 x 3, totalizando 9 tratamentos com 5 repeticoes de oito aves cada. Os fatores estudados foram niveis de oleo de soja (1, 2 e 3%) e niveis de energia metabolizavel na racao (2600, 2750 e 2900 kcal/kg). Nao foi observada interacao entre os niveis do oleo de soja e a energia metabolizavel da dieta. Os niveis de oleo de soja nao promoveram efeito para nenhum dos parâmetros avaliados. Os niveis de energia metabolizavel nao influenciaram a producao de ovos, conversao alimentar, massa de ovos, peso de gema e de casca, percentagem de gema e de clara e gravidade especifica. Foram observadas diferencas para conversao energetica, peso do ovo e de clara, e percentagem de casca, sendo os maiores pesos observados no menor nivel de energia e a maior percentagem observada no maior nivel de energia. O fornecimento de 2600 kcal de EM/kg foi mais adequado para manter a producao e o peso corporal e para promover melhoria na conversao energetica e aumento no peso do ovo.

[Pregnancy in adolescence: reason and perceptions of adolescents].

Abstract: This research encompasses the capital Aracaju, Sergipe, and its surroundings health districts. It was dealt from October to December 2002 with 216 pregnant adolescents assisted by the Strategy of the Family Health Program. Its main goal is to raise and identify the social-demographic and the gynecological profile of those young ladies, by identifying the reason which led them to pregnancy and by knowing their pereption of it. The vast majority of them are 17, colored, literate, house-wives who have one single steady partner and survive with less than the minimum wage. Among those 32.4% got their first period by the age of 12; 25% had their first sexual intercourse by the age of 16; anf 72.7% had their first gestation. The reason which stood out from the others was the will to become a mother, for their perception towards pregnancy is related to happiness and personal achievement.

O processo de erosão em ambiente de superficie sertaneja - varjota (ce)

Abstract: The present objective assay to analyze the erosion of the ground in surface environment sertaneja of the half-barren person from the state of Ceara. The city of Varjota was the study object, where three experimental areas under different uses of handling of the ground had been installed.