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Showing papers by "Universidade Federal de Mato Grosso published in 2017"


Journal ArticleDOI
TL;DR: The present guidelines aims to update the first Brazilian consensus on paracoccidioidomycosis by providing evidence-based recommendations for bedside patient management and emphasis on clinical, microbiological, and serological diagnosis and management of clinical forms and sequelae, as well as in patients with comorbidities and immunosuppression.
Abstract: Paracoccidioidomycosis is a systemic fungal disease occurring in Latin America that is associated with rural environments and agricultural activities. However, the incidence and prevalence of paracoccidiodomycosis is underestimated because of the lack of compulsory notification. If paracoccidiodomycosis is not diagnosed and treated early and adequately, the endemic fungal infection could result in serious sequelae. While the Paracoccidioides brasiliensis ( P. brasiliensis ) complex has been known to be the causal agent of paracoccidiodomycosis, a new species, Paracoccidioides lutzii ( P. lutzii ), has been reported in Rondonia, where the disease has reached epidemic levels, and in the Central West and Para. Accurate diagnoses and availability of antigens that are reactive with the patients' sera remain significant challenges. Therefore, the present guidelines aims to update the first Brazilian consensus on paracoccidioidomycosis by providing evidence-based recommendations for bedside patient management. This consensus summarizes etiological, ecoepidemiological, molecular epidemiological, and immunopathological data, with emphasis on clinical, microbiological, and serological diagnosis and management of clinical forms and sequelae, as well as in patients with comorbidities and immunosuppression. The consensus also includes discussion of outpatient treatments, severe disease forms, disease prevalence among special populations and resource-poor settings, a brief review of prevention and control measures, current challenges and recommendations.

252 citations


Journal ArticleDOI
TL;DR: In this paper, a distribuicao espacial da area plantada de lavouras, consumo de agrotoxicos and agravos a saude relacionados, como estrategia de Vigilância em Saude, was presented.
Abstract: Resumo O uso de agrotoxicos na agricultura brasileira e um problema de saude publica, dadas as contaminacoes no ambiente, em alimentos e as intoxicacoes na saude humana. Objetivou-se apresentar a distribuicao espacial da area plantada de lavouras, consumo de agrotoxicos e agravos a saude relacionados, como estrategia de Vigilância em Saude. Obteve-se dados de area plantada de 21 culturas predominantes, indicadores de consumo de agrotoxicos por hectare para cada cultura e agravos a saude. Espacializou-se o consumo de agrotoxicos nos municipios brasileiros e correlacionou-se as incidencias de intoxicacoes por agrotoxicos: aguda, subaguda e cronica. Constatou-se predominio dos cultivos de soja, milho e cana, que juntos corresponderam a 76% da area plantada no Brasil em 2015. Pulverizou-se 899 milhoes de litros de agrotoxicos nessas lavouras, com Mato Grosso, Parana e Rio Grande Sul tendo utilizado as maiores quantidades. Os agravos a saude apresentaram correlacoes positivas e significativas com o uso de agrotoxicos. A estrategia metodologica possibilitou identificar municipios prioritarios para a Vigilância em Saude e o desenvolvimento de acoes intersetoriais de prevencao e mitigacao dos impactos dos agrotoxicos na saude e ambiente.

248 citations


Journal ArticleDOI
TL;DR: A meta-analysis found that silage inoculation with LAB markedly increased silage fermentation and dry matter recovery in temperate and tropical grasses, alfalfa, and other legumes and increased milk yield and the response had low heterogeneity, but inoculation had no effect on diet digestibility and feed efficiency.

210 citations


Journal ArticleDOI
TL;DR: Effect of adding Escherichia coli O157:H7 with or without chemical or microbial additives on the bacterial diversity and composition of alfalfa silage and associations between the relative abundance of known and unknown bacterial species and indices of silage fermentation quality were examined.

164 citations


Journal ArticleDOI
TL;DR: In this paper, the authors expose the situation and illustrate how national policies constitute the main threat to freshwater fish biodiversity and explain that the most devastating, pervasive and systemic threats are rooted in official policies, particularly unsustainable activities (e.g., hydropower, water diversion, mining, aquaculture, agriculture and fishing), poor management/conservation, and harmful legislation.
Abstract: Neotropical freshwater fishes are the most diverse on the planet (>5,500 species), although nations in Latin America have been negligent regarding their conservation National policies have historically encouraged unsustainable practices, and recent decades have witnessed a sharp increase in harmful activities Our aim with this review was to expose this situation and illustrate how national policies constitute the main threat to freshwater fish biodiversity We explain that the most devastating, pervasive and systemic threats are rooted in official policies, particularly unsustainable activities (eg hydropower, water diversion, mining, aquaculture, agriculture and fishing), poor management/conservation (eg fish stocking and passages) and harmful legislation (eg poor licensing, non-native species) We provide a broad portrait of the Neotropical scenario, where unsustainable policies have caused considerable damage to freshwater ecosystems, and focus on major examples from Brazil, where development projects have caused large-scale losses to fish biodiversity Such modus operandi of human development is incompatible with the persistence of biodiversity, and no simple solution is available to correct or minimize its effects The current situation demands a profound behavioural shift towards better practices and policies, or these multiple high-impact activities will continue eroding freshwater fish biodiversity and impairing essential ecosystem services

156 citations


Journal ArticleDOI
TL;DR: This study aimed to identify and document species of medicinal plants used by local experts from riverine communities in the North Araguaia microregion in Mato Grosso State, and to further chemical and pharmacological studies on species selected based on searches in the relevant literature.

152 citations


Journal ArticleDOI
TL;DR: Itraconazole and the trimethoprim-sulfamethoxazole combination was compared regarding efficacy, effectiveness and safety, demonstrating that azole should be the first choice in the treatment of paracoccidioidomycosis.
Abstract: Background This review article summarizes and updates the knowledge on paracoccidioidomycosis. P lutzii and the cryptic species of P. brasiliensis and their geographical distribution in Latin America, explaining the difficulties observed in the serological diagnosis. Objectives Emphasis has been placed on some genetic factors as predisposing condition for paracoccidioidomycosis. Veterinary aspects were focused, showing the wide distribution of infection among animals. The cell-mediated immunity was better characterized, incorporating the recent findings. Methods Serological methods for diagnosis were also compared for their parameters of accuracy, including the analysis of relapse. Results Clinical forms have been better classified in order to include the pictures less frequently observesiod. Conclusion Itraconazole and the trimethoprim-sulfamethoxazole combination was compared regarding efficacy, effectiveness and safety, demonstrating that azole should be the first choice in the treatment of paracoccidioidomycosis.

103 citations


Journal ArticleDOI
TL;DR: Current knowledge on Nrf2 signaling pathway structure and activity under both physiological state and upon oxidative stress is summarized, highlighting the potential therapeutic effects of diverse natural and synthetic compounds as NRF2 regulators.

103 citations


Journal ArticleDOI
TL;DR: Mechanisms linking Angiotensin II and the innate immune system, in the pathogenesis of hypertension, are discussed.

92 citations


Journal ArticleDOI
TL;DR: A parallelism between daily consumption of meals with healthier eating and greater adherence to traditional Brazilian food habits was showed, suggesting the adoption of regular meal habits may help adolescents improve their diet quality.

67 citations



Journal ArticleDOI
TL;DR: Overall, L. fermentum SJRP30 and L. casei SJRP145 and SJRP146 were revealed to be safe and to possess similar or superior probiotic characteristics compared to the reference strain L. rhamnosus GG (ATCC 53103).
Abstract: The aim of this study was to evaluate the safety and probiotic potential characteristics of ten Lactobacillus spp. strains (Lactobacillus fermentum SJRP30, Lactobacillus casei SJRP37, SJRP66, SJRP141, SJRP145, SJRP146, and SJRP169, and Lactobacillus delbrueckii subsp. bulgaricus SJRP50, SJRP76, and SJRP149) that had previously been isolated from water buffalo mozzarella cheese. The safety of the strains was analyzed based on mucin degradation, hemolytic activity, resistance to antibiotics and the presence of genes encoding virulence factors. The in vitro tests concerning probiotic potential included survival under simulated gastrointestinal (GI) tract conditions, intestinal epithelial cell adhesion, the presence of genes encoding adhesion, aggregation and colonization factors, antimicrobial activity, and the production of the β-galactosidase enzyme. Although all strains presented resistance to several antibiotics, the resistance was limited to antibiotics to which the strains had intrinsic resistance. Furthermore, the strains presented a limited spread of genes encoding virulence factors and resistance to antibiotics, and none of the strains presented hemolytic or mucin degradation activity. The L. delbrueckii subsp. bulgaricus strains showed the lowest survival rate after exposure to simulated GI tract conditions, whereas all of the L. casei and L. fermentum strains showed good survivability. None of the tested lactobacilli strains presented bile salt hydrolase (BSH) activity, and only L. casei SJRP145 did not produce the β-galactosidase enzyme. The strains showed varied levels of adhesion to Caco-2 cells. None of the cell-free supernatants inhibited the growth of pathogenic target microorganisms. Overall, L. fermentum SJRP30 and L. casei SJRP145 and SJRP146 were revealed to be safe and to possess similar or superior probiotic characteristics compared to the reference strain L. rhamnosus GG (ATCC 53103).

Journal ArticleDOI
TL;DR: In this paper, the authors analyze the consequences of such cuts on the Research Program on Biodiversity (PPBio), the largest biodiversity research network in Brazil (626 researchers, nine networks in all Brazilian biomes).

Journal ArticleDOI
13 Jun 2017-PLOS ONE
TL;DR: Overall, the results confirm that conversion of cerrado vegetation to pasture causes soil hydro-physical properties deterioration, reduction in evapotranspiration reduction, and increased streamflow.
Abstract: Understanding the impacts of land-use change on landscape-hydrological dynamics is one of the main challenges in the Northern Brazilian Cerrado biome, where the Amazon agricultural frontier is located. Motivated by the gap in literature assessing these impacts, we characterized the soil hydro-physical properties and quantified surface water fluxes from catchments under contrasting land-use in this region. We used data from field measurements in two headwater micro-catchments with similar physical characteristics and different land use, i.e. cerrado sensu stricto vegetation and pasture for extensive cattle ranching. We determined hydraulic and physical properties of the soils, applied ground-based remote sensing techniques to estimate evapotranspiration, and monitored streamflow from October 2012 to September 2014. Our results show significant differences in soil hydro-physical properties between the catchments, with greater bulk density and smaller total porosity in the pasture catchment. We found that evapotranspiration is smaller in the pasture (639 ± 31% mm yr-1) than in the cerrado catchment (1,004 ± 24% mm yr-1), and that streamflow from the pasture catchment is greater with runoff coefficients of 0.40 for the pasture and 0.27 for the cerrado catchment. Overall, our results confirm that conversion of cerrado vegetation to pasture causes soil hydro-physical properties deterioration, reduction in evapotranspiration reduction, and increased streamflow.

Journal ArticleDOI
TL;DR: The aim is to summarize the mechanisms by which melatonin may modulate the deteriorating effects of AlP poisoning on cardiac mitochondria, and to recommend exogenous drugs as an effective therapy.
Abstract: Objectives Acute poisoning with aluminium phosphide (AlP) is a major cause of mortality in developing countries. AlP mortality is due to cardiac dysfunction leading to cardiomyocyte death. The main mechanism is an inhibition of cytochrome c oxidase in the cardiomyocyte mitochondria, resulting in a decreased ATP production and oxidative stress. Unfortunately, the administration of exogenous drugs does not meet the desired requirements of an effective therapy. Melatonin is an amphiphilic molecule and can easily pass through all cellular compartments with the highest concentration recorded in mitochondria. It is known as a vigorous antioxidant, acting as a potent reactive oxygen species (ROS) scavenger. Our aim is to summarize the mechanisms by which melatonin may modulate the deteriorating effects of AlP poisoning on cardiac mitochondria. Key findings Melatonin not only mitigates the inhibition of respiratory chain complexes, but also increases ATP generation. Moreover, it can directly inhibit the mitochondrial permeability transition pore (mPTP) opening, thus preventing apoptosis. In addition, melatonin inhibits the release of cytochrome c from mitochondria to hinder caspase activation leading to cell survival. Summary Based on the promising effects of melatonin on mitochondria, melatonin may mitigate AlP-induced cardiotoxicity and might be potentially suggested as cardioprotective in AlP-intoxicated patients.

Journal ArticleDOI
TL;DR: In this article, the authors show that jaguar ecotourism represents a gross annual income of US$6,827,392 in land-use revenue across a representative portion of the Brazilian Pantanal, the world's largest wetland.

Journal ArticleDOI
TL;DR: It is concluded that tropical fish FD is highly vulnerable, which implies that conservation efforts for freshwater fishes should emphasize temperate regions.
Abstract: Here we extend a discussion initiated by Toussaint et al. (Sci Rep 6:22125, 2016) concerning the relationship between global patterns of freshwater fish functional diversity (FD) and its vulnerability to human impacts. Based on a set of morphological traits, they concluded that Neotropical freshwater fishes have highest FD, but low vulnerability given high levels of functional redundancy. This conclusion implies that conservation efforts for freshwater fishes should emphasize temperate regions. This perspective is risky, because Toussaint et al.’s study seriously underestimates the full scope of FD, including important ecosystem services provided by fishes in the tropics. We briefly discuss some additional and well-documented aspects of tropical freshwater fish FD and conclude that tropical fish FD is highly vulnerable.

Journal ArticleDOI
TL;DR: This study revealed extensive genetic diversity among Brazilian pestivirus isolates, and the combination of pestiviruses that was detected is unique to Brazil.
Abstract: Pestivirus infections in ruminants result in significant economic losses worldwide. The aetiological agents are three species from the genus Pestivirus, family Flaviviridae, including bovine viral diarrhoea virus type 1 (BVDV-1), BVDV-2, border disease virus (BDV), and an atypical pestivirus named HoBi-like pestivirus. In this study, eighty-nine pestivirus isolates that were collected in Brazil between 1995 and 2014 and that originated from either cattle, fetal bovine serum (FBS) or as cell culture contaminants were genotyped based on a comparison of gene sequences from their 5' untranslated regions (5'UTR), N-terminal autoprotease (Npro ) and envelope glycoprotein 2 (E2). Of these isolates, 53.9% of the sequences were genotyped as BVDV-1, 33.7% as BVDV-2 and 12.4% as HoBi-like pestivirus. The prevalence of subgenotypes within the species was as follows: BVDV-1a (35.9%), BVDV-2b (31.4%), BVDV-1b (10.1%), BVDV-1d (6.7%), BVDV-2c (2.2%) and BVDV-1e (1.1%). BVDV-2c and BVDV-1e were detected for the first time in Brazil. This study revealed extensive genetic diversity among Brazilian pestivirus isolates, and the combination of pestiviruses that was detected is unique to Brazil. This information may serve as a foundation for designing and evaluating diagnostic tools and in the development of more effective vaccines; therefore, it may potentially contribute to pestivirus control and eradication.

Journal ArticleDOI
TL;DR: It is found that Roundup Original® is able to trigger important changes in the biochemical parameters tested, showing it can be a potential threat for the health and survival of fish in the environment.

Journal ArticleDOI
TL;DR: Applying exogenous fibrolytic enzymes to dairy cow diets tended to increase dry matter digestibility and NDFD and increased milk yield by relatively small amounts, reflecting the variable response among EFE types.

Journal ArticleDOI
TL;DR: Analysis of the ehrlichial trp36 polymorphic gene in 10 samples revealed substantial polymorphism among the E. canis genotypes, including divergent tandem repeat sequences.

Journal ArticleDOI
TL;DR: The data suggest an apparent role for the ruminal bacterial community as a determinant of MPE, as both liquid- and solids-associated BCC differed between individuals within a pair before contents exchange and after exchange.

Journal ArticleDOI
TL;DR: This is the first report on the in vitro schistosomicidal and leishmanicidal activities of the EtOAc extract of A. terreus—F7 and compounds 1, 2, and 3; and the antitumor activity of compounds 2 and 3 against MDA-MB-231 and MCF-7 cells.
Abstract: The compounds terrein (1), butyrolactone I (2), and butyrolactone V (3) were isolated from the ethyl acetate extract (EtOAc) of the endophytic fungus Aspergillus terreus—F7 obtained from Hyptis suaveolens (L.) Poit. The extract and the compounds presented schistosomicidal activity against Schistosoma mansoni; at 100 µg/mL for EtOAc extract, 1297.3 µM for compound 1, 235.6 µM for compound 2, and 454.1 µM for compound 3, they killed 100% of the parasites after 72 h of treatment. Compounds 1, 2, and 3 exerted moderate leishmanicidal activity against Leishmania amazonensis (IC50 ranged from 23.7 to 78.6 µM). At 235.6 and 227.0 µM, compounds 2 and 3, respectively, scavenged 95.92 and 95.12% of the DPPH radical (2,2-diphenyl-1-picryl-hydrazyl), respectively. Regarding the cytotoxicity against the breast tumor cell lines MDA-MB-231 and MCF-7, compound 2 gave IC50 of 34.4 and 17.4 µM, respectively, while compound 3 afforded IC50 of 22.2 and 31.9 µM, respectively. At 117.6 µM, compound 2 inhibited the growth of and killed the pathogen Escherichia coli (ATCC 25922). Compounds 1, 2, and 3 displayed low toxicity against the normal line of human lung fibroblasts (GM07492A cells), with IC50 of 15.3 × 103, 3.4 × 103, and 5.8 × 103 µM, respectively. This is the first report on (i) the in vitro schistosomicidal and leishmanicidal activities of the EtOAc extract of A. terreus—F7 and compounds 1, 2, and 3; and (ii) the antitumor activity of compounds 2 and 3 against MDA-MB-231 and MCF-7 cells.

Journal ArticleDOI
TL;DR: It was found that 9.9% of the students had suicidal thoughts in the previous 30 days and the variables economic class, sexual orientation, religious practice, suicide attempts in the family and among friends, alcohol consumption and depressive symptoms were associated with suicidal ideation.
Abstract: Objetivo: analizar los factores asociados a la ideacion suicida en una muestra representativa de estudiantes universitarios Metodo: estudio transversal analitico, realizado con 637 estudiantes en una Universidad Federal de Mato Grosso Fueron investigadas las variables: presencia de ideacion suicida; demograficas y socioeconomicas; uso de alcohol por medio del Alcohol, Smoking and Substance Involvement Screening Test; y sintomas depresivos (Inventario de Depresion Mayor) El analisis bivariado fue realizado con el test de Chi-cuadrado y el analisis multiple con el modelo de regresion de Poisson Resultados: se constato que 9,9% de los estudiantes tuvieron ideas suicidas en los ultimos 30 dias, y en el analisis bivariado las variables: clase economica; orientacion sexual; practica religiosa; intentos de suicidio en la familia y entre amigos; consumo de alcohol; y sintomas depresivos, presentaron asociacion con ideacion suicida En el analisis multiple permanecieron como factores asociados la orientacion sexual, los intentos de suicidio en la familia y la presencia de sintomas depresivos Conclusion: estos hallazgos constituyen un diagnostico situacional que posibilita la formulacion de politicas academicas y de acciones de prevencion para enfrentar esa situacion en el campus universitario

Journal ArticleDOI
TL;DR: In this paper, the authors found that the homogenizing effect of flooding and the environmental differentiation during the dry period, as reflected in fish communities, are related to the type of connection between floodplain lakes and rivers.
Abstract: We evaluated if the homogenizing effect of flooding and the environmental differentiation during the dry period, as reflected in fish communities, are related to the type of connection between floodplain lakes and rivers. In order to do this, we sampled fish in the early dry and the early flood hydroperiods. We estimated the fish assemblage structure, as fish species richness, abundance, biomass, and beta diversity. We found that biomass varied among connectivity levels and hydroperiods, while richness varied among hydroperiods and density was influenced by an interaction between connectivity and hydroperiod. Species composition varied as a function of both connection type and hydroperiod, but the interaction between these factors was not observed. The fish assemblage in isolated lakes differed from those in temporarily and permanently connected lakes. Beta diversity increased from the early dry to the early flood period and was higher in temporarily connected lakes than in permanently connected lakes. These results indicated that the spatio-temporal variations in local fish communities of floodplain lakes can be attributed to differences in connectivity. Consequently, they strengthen the hypothesis that dispersal plays a fundamental role in shaping the pattern of fish species diversity in floodplain lakes.

Journal ArticleDOI
TL;DR: There is a lack both in the amount and impact of research conducted in Latin America on vascular disorders related to problems during pregnancy, and the need for increased effective regional and international collaboration to generate new data specific to this region is related.
Abstract: Pregnancy is a physiologically stressful condition that generates a series of functional adaptations by the cardiovascular system. The impact of pregnancy on this system persists from conception beyond birth. Recent evidence suggests that vascular changes associated with pregnancy complications, such as preeclampsia, affect the function of the maternal and offspring vascular systems, after delivery and into adult life. Since the vascular system contributes to systemic homeostasis, defective development or function of blood vessels predisposes both mother and infant to future risk for chronic disease. These alterations in later life range from fertility problems to alterations in the central nervous system or immune system, among others. It is important to note that rates of morbi-mortality due to pregnancy complications including preeclampsia, as well as cardiovascular diseases, have a higher incidence in Latin-American countries than in more developed countries. Nonetheless, there is a lack both in the amount and impact of research conducted in Latin America. An impact, although smaller, can be seen when research in vascular disorders related to problems during pregnancy is analyzed. Therefore, in this review, information about preeclampsia and endothelial dysfunction generated from research groups based in Latin-American countries will be highlighted. We relate the need, as present in many other countries in the world, for increased effective regional and international collaboration to generate new data specific to our region on this topic.

Proceedings ArticleDOI
01 Jan 2017
TL;DR: Current literature on software ecosystem governance is summarized by framing prevalent definitions, classifying governance mechanisms, and proposing a research agenda.
Abstract: The field of Software ecosystems is a growing discipline that has been investigated from managerial, social, and technological perspectives. The governance of software ecosystems requires a careful balance of control and autonomy given to players. Orchestrators that are able to balance their own interests by bringing joint benefits for other players are likely to create healthy ecosystems. Selecting appropriate governance mechanisms is a key problem involved in the management of proprietary and open source ecosystems. This article summarizes current literature on software ecosystem governance by framing prevalent definitions, classifying governance mechanisms, and proposing a research agenda. We performed a systematic literature review of 63 primary studies. Several studies describe governance mechanisms, which were classified in three categories: value creation, coordination of players, and organizational openness and control. The number of studies indicates that the domain of software ecosystems and their governance is maturing. However, further studies are needed to address central challenges involved on the implementation of appropriate governance mechanisms that can nurture the health of ecosystems. We present a research agenda with several opportunities for researchers and practitioners to explore these issues.

Journal ArticleDOI
TL;DR: T-I pretreatment significantly protected mitochondria against PQ-induced redox impairment through an Nrf2-dependent mechanism involving upregulation of antioxidant enzymes, such as Mn-superoxide dismutase (Mn-SOD), glutathione peroxidase (GPx), and both catalytic and modifier subunits of γ-glutamate-cysteine ligase (γ-GCL).
Abstract: Tanshinone I (T-I; 1,6-Dimethylnaphtho[1,2-g][1]benzofuran-10,11-dione; C18H12O3), which may be found in Salvia miltiorrhiza Bunge (Danshen), is a potent anti-inflammatory, antioxidant, and anti-cancer agent. At least in part, T-I exerts antioxidant activity by activating signaling pathways associated with the maintenance of the redox state in mammalian cells. In this context, the upregulation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) has received attention regarding the role of this transcription factor in modulating the expression of antioxidant enzymes and the metabolism of glutathione (GSH). Even though there is a growing body of evidence suggesting that T-I mediates protection against several pro-oxidant challenges in both in vitro and in vivo experimental models, it remains to be examined whether and how T-I would modulate mitochondrial function during redox disturbances. Therefore, we aimed to reveal whether T-I would exhibit protective effects on mitochondria of SH-SY5Y cells treated with paraquat (PQ), a well-known mitochondrial toxic agent. We found that T-I pretreatment significantly protected mitochondria against PQ-induced redox impairment through an Nrf2-dependent mechanism involving upregulation of antioxidant enzymes, such as Mn-superoxide dismutase (Mn-SOD), glutathione peroxidase (GPx), and both catalytic and modifier subunits of γ-glutamate-cysteine ligase (γ-GCL). T-I prevented complex I and mitochondrial membrane potential (MMP) impairments elicited by PQ. Thus, T-I may be viewed as a new mitochondrial protective agent whose complete mechanism of action needs to be investigated, but it seems to involve mitochondriotropic aspects related to the chemistry of this molecule.

Journal ArticleDOI
TL;DR: Results support that areas converted for livestock production retain only a subset of the Neotropical bat functional diversity while most of the functional groups are negatively impacted, and heterogeneity of responses seems critical for the understanding of the effects of land use change on animal communities.

Journal ArticleDOI
TL;DR: A paper-based immunoassay for the detection of the carcinoembryonic antigen (CEA) and performed a statistical assessment to establish the assay's cut-off value using the Youden's J index, highlighting the importance of statistical tools to evaluate a new low-cost diagnostic method.