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Universidade Federal de Viçosa

EducationViçosa, Brazil
About: Universidade Federal de Viçosa is a education organization based out in Viçosa, Brazil. It is known for research contribution in the topics: Population & Dry matter. The organization has 16012 authors who have published 26711 publications receiving 353416 citations.


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Journal ArticleDOI
TL;DR: Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation.
Abstract: Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). Although the protein-coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation. The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some taxonomic groups, such as the early diverging lineages and the ascomycete yeasts, but was otherwise slightly inferior to the ITS. The nuclear ribosomal small subunit has poor species-level resolution in fungi. ITS will be formally proposed for adoption as the primary fungal barcode marker to the Consortium for the Barcode of Life, with the possibility that supplementary barcodes may be developed for particular narrowly circumscribed taxonomic groups.

4,116 citations

Journal ArticleDOI
TL;DR: GENES as mentioned in this paper is a software package used for data analysis and processing with different biometric models and is essential in genetic studies applied to plant and animal breeding It allows parameter estimation to analyze biological phenomena and is fundamental for the decision-making process and predictions of success and viability of selection strategies.
Abstract: GENES is a software package used for data analysis and processing with different biometric models and is essential in genetic studies applied to plant and animal breeding It allows parameter estimation to analyze biological phenomena and is fundamental for the decision-making process and predictions of success and viability of selection strategies The program can be downloaded from the Internet (http://wwwufvbr/dbg/genes/geneshtm or http://wwwufvbr/dbg/biodatahtm) and is available in Portuguese, English and Spanish Specific literature (http://wwwlivrariaufvbr/) and a set of sample files are also provided, making GENES easy to use The software is integrated into the programs MS Word, MS Excel and Paint, ensuring simplicity and effectiveness in data import and export of results, figures and data It is also compatible with the free software R and Matlab, through the supply of useful scripts available for complementary analyses in different areas, including genome wide selection, prediction of breeding values and use of neural networks in genetic improvement

1,190 citations

Journal ArticleDOI
TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.
Abstract: In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

1,129 citations

Journal ArticleDOI
TL;DR: In this article, the effects of NaCl stress on the activity of antioxidant enzymes such as superoxide dismutase (SOD: EC 1.15.1), peroxidase (POD:EC 1.11.7), and glutathione reductase (GR: EC1.6.4) were investigated in two cotton cultivars, Guazuncho and Pora, grown in nutrient solution.

1,085 citations

Journal ArticleDOI
TL;DR: The main synthesis methods of ZnO nanoparticles, principal characteristics and mechanisms of antimicrobial action as well as the effect of their incorporation in polymeric matrices are discussed in this paper.
Abstract: Zinc oxide (ZnO) is an inorganic compound widely used in everyday applications. ZnO is currently listed as a generally recognized as safe (GRAS) material by the Food and Drug Administration and is used as food additive. The advent of nanotechnology has led the development of materials with new properties for use as antimicrobial agents. Thus, ZnO in nanoscale has shown antimicrobial properties and potential applications in food preservation. ZnO nanoparticles have been incorporated in polymeric matrices in order to provide antimicrobial activity to the packaging material and improve packaging properties. This review presents the main synthesis methods of ZnO nanoparticles, principal characteristics and mechanisms of antimicrobial action as well as the effect of their incorporation in polymeric matrices. Safety issues such as exposure routes and migration studies are also discussed.

977 citations


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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
202346
2022320
20212,074
20202,208
20191,941
20181,865