Showing papers by "Université catholique de Louvain published in 1998"

The Book of Involutions

Abstract: This monograph yields a comprehensive exposition of the theory of central simple algebras with involution, in relation with linear algebraic groups It aims to provide the algebra-theoretic foundations for much of the recent work on linear algebraic groups over arbitrary fields Involutions are viewed as twisted forms of similarity classes of hermitian or bilinear forms, leading to new developments on the model of the algebraic theory of quadratic forms Besides classical groups, phenomena related to triality are also discussed, as well as groups of type F_4 or G_2 arising from exceptional Jordan or composition algebras Several results and notions appear here for the first time, notably the discriminant algebra of an algebra with unitary involution and the algebra-theoretic counterpart to linear groups of type D_4 For research mathematicians and graduate students working in central simple algebras, algebraic groups, nonabelian Galois cohomology or Jordan algebras

A gene encoding a liver-specific ABC transporter is mutated in progressive familial intrahepatic cholestasis

Abstract: The progressive familial intrahepatic cholestases (PFIC) are a group of inherited disorders with severe cholestatic liver disease from early infancy. A subgroup characterized by normal serum cholesterol and gamma-glutamyltranspeptidase (gammaGT) levels is genetically heterogeneous with loci on chromosomes 2q (PFIC2) and 18q. The phenotype of the PFIC2-linked group is consistent with defective bile acid transport at the hepatocyte canalicular membrane. The PFIC2 gene has now been identified by mutations in a positional candidate, BSEP, which encodes a liver-specific ATP-binding cassette (ABC) transporter, sister of p-glycoprotein (SPGP). The product of the orthologous rat gene has been shown to be an effective bile acid transporter in vitro. These data provide evidence that SPGP is the human bile salt export pump (BSEP).

The Virulence Plasmid of Yersinia, an Antihost Genome

Abstract: The 70-kb virulence plasmid enables Yersinia spp. (Yersinia pestis, Y. pseudotuberculosis, and Y. enterocolitica) to survive and multiply in the lymphoid tissues of their host. It encodes the Yop virulon, an integrated system allowing extracellular bacteria to disarm the cells involved in the immune response, to disrupt their communications, or even to induce their apoptosis by the injection of bacterial effector proteins. This system consists of the Yop proteins and their dedicated type III secretion apparatus, called Ysc. The Ysc apparatus is composed of some 25 proteins including a secretin. Most of the Yops fall into two groups. Some of them are the intracellular effectors (YopE, YopH, YpkA/YopO, YopP/YopJ, YopM, and YopT), while the others (YopB, YopD, and LcrV) form the translocation apparatus that is deployed at the bacterial surface to deliver the effectors into the eukaryotic cells, across their plasma membrane. Yop secretion is triggered by contact with eukaryotic cells and controlled by proteins of the virulon including YopN, TyeA, and LcrG, which are thought to form a plug complex closing the bacterial secretion channel. The proper operation of the system also requires small individual chaperones, called the Syc proteins, in the bacterial cytosol. Transcription of the genes is controlled both by temperature and by the activity of the secretion apparatus. The virulence plasmid of Y. enterocolitica and Y. pseudotuberculosis also encodes the adhesin YadA. The virulence plasmid contains some evolutionary remnants including, in Y. enterocolitica, an operon encoding resistance to arsenic compounds.

The Bifidogenic Nature of Chicory Inulin and Its Hydrolysis Products

Abstract: Research data on the bifidogenic effect of beta(2-1)fructans, which at present are commercialized in the U.S., Japan and Europe as food ingredients, are presented. These food ingredients originate from two different sources. Short-chain fructo-oligosaccharides are synthesized from sucrose and are composed of GFn [n beta(2-1) linked fructose moieties bound to a glucose molecule; 2 10 are fermented on average half as quickly as molecules with a DP < 10. All beta(2-1)fructans are bifidogenic and classified as biobiotics.

Managing conflicts in goal-driven requirements engineering

Abstract: A wide range of inconsistencies can arise during requirements engineering as goals and requirements are elicited from multiple stakeholders. Resolving such inconsistencies sooner or later in the process is a necessary condition for successful development of the software implementing those requirements. The paper first reviews the main types of inconsistency that can arise during requirements elaboration, defining them in an integrated framework and exploring their interrelationships. It then concentrates on the specific case of conflicting formulations of goals and requirements among different stakeholder viewpoints or within a single viewpoint. A frequent, weaker form of conflict called divergence is introduced and studied in depth. Formal techniques and heuristics are proposed for detecting conflicts and divergences from specifications of goals/requirements and of domain properties. Various techniques are then discussed for resolving conflicts and divergences systematically by the introduction of new goals or by transforming the specifications of goals/objects toward conflict-free versions. Numerous examples are given throughout the paper to illustrate the practical relevance of the concepts and techniques presented. The latter are discussed in the framework of the KAOS methodology for goal-driven requirements engineering.

Expression of full-length utrophin prevents muscular dystrophy in mdx mice

Abstract: Duchenne muscular dystrophy (DMD) is a lethal, progressive muscle wasting disease caused by a loss of sarcolemmal bound dystrophin, which results in the death of the muscle fiber leading to the gradual depletion of skeletal muscle. The molecular structure of dystrophin is very similar to that of the related protein utrophin. Utrophin is found in all tissues and is confined to the neuromuscular and myotendinous junctions in mature muscle. Sarcolemmal localization of a truncated utrophin transgene in the dystrophin-deficient mdx mouse significantly improves the dystrophic muscle phenotype. Therefore, up-regulation of utrophin by drug therapy is a plausible therapeutic approach in the treatment of DMD. Here we demonstrate that expression of full-length utrophin in mdx mice prevents the development of muscular dystrophy. We assessed muscle morphology, fiber regeneration and mechanical properties (force development and resistance to stretch) of mdx and transgenic mdx skeletal and diaphragm muscle. The utrophin levels required in muscle are significantly less than the normal endogenous utrophin levels seen in lung and kidney, and we provide evidence that the pathology depends on the amount of utrophin expression. These results also have important implications for DMD therapies in which utrophin replacement is achieved by delivery using exogenous vectors.

Magnitude and Time Course of Microvascular Obstruction and Tissue Injury After Acute Myocardial Infarction

Abstract: Background—Microvascular obstruction within an area of myocardial infarction indicates worse functional recovery and a higher risk of postinfarction complications. After prolonged coronary occlusio...

Dynamical atomic charges: The case of ABO(3) compounds

Abstract: Based on recent first-principles computations in perovskite compounds, especially BaTiO3, we examine the significance of the Born effective charge concept and contrast it with other atomic charge definitions, either static (Mulliken, Bader, etc.) or dynamical (Callen, Szigeti, etc.). It is shown that static and dynamical charges are not driven by the same underlying parameters. A unified treatment of dynamical charges in periodic solids and large clusters is proposed. The origin of the difference between static and dynamical charges is discussed in terms of local polarizability and delocalized transfers of charge: local models succeed in reproducing anomalous effective charges thanks to large atomic polarizabilities but, in ABO(3) compounds, ab initio calculations favor the physical picture based upon transfer of charges. Various results concerning barium and strontium titanates are presented. The origin of anomalous Born effective charges is discussed thanks to a band-by-band decomposition which allows us to identify the displacement of the Wannier center of separated bands induced by an atomic displacement. The sensitivity of the Born effective charges to microscopic and macroscopic strains is examined. Finally, we estimate the spontaneous polarization in the four phases of barium titanate.

Semidefinite relaxation and nonconvex quadratic optimization

Abstract: In this paper we study the quality of semidefinite relaxation for a global quadratic optimization problem with diagonal quadratic consraints. We prove that such relaxation approximates the exact solution of the problem with relative accuracy mu = (pi/2)-1. We consider some applications of this result.

Relationship between soil chemical factors and grassland diversity

Abstract: Many studies carried out during these last few years have focused on the factors influencing plant diversity in species-rich grasslands. This is due to the fact that these ecosystems, among the most diversified in temperate climates, are extremely threatened; in some areas, they have almost disappeared. The re-establishment of these habitats implies to know the living conditions of the associations to be recreated. Very often, the typical species of these communities have become so rarefied that the seed bank or the seed rain are not sufficient to recreate the plant community. Most of the time, to achieve the restoration of these communities, they have to be totally recreated by sowing. For the restoration or the maintenance of the community, the soil chemical characteristics have also to be appropriate or if not modified. This research tends to establish a relation between some soil chemical factors and the plant diversity of a great number of stations. This research has illuminated the relationship between soil extractable phosphorus and potassium and plant diversity. Over 5 mg of phosphorus per 100 g of dry soil (acetate + EDTA extraction), no station containing more than 20 species per 100 m(2) has been found. The highest number of species is found below the optimum content of the soil for plant nutrition (5-8 mg P/100 g). Concerning the potassium, the highest number of species is found at 20 mg/100, a value corresponcing to an optimum content of the soil for plant nutrition. High potassium contents, in opposition to phosphorus contents, are thus compatible with high values of diversity. Other factors (i.e. pH, organic matter, total nitrogen and calcium) do not show so clearly a relation with plant diversity. Excess of N-NO3 is known for its negative effect on the diversity of plant communities. In these environments, apart from the atmospheric deposits which can be important in some areas, N-NO3 is derived mainly from the symbiotic fixation of atmospheric nitrogen by legumes as well as from the mineralization of the organic matter of the soil. It is possible that, when in small quantities, the available soil phosphorus could be a limiting factor of the N-NO3 supply by these two sources. In this hypothesis, nitrogen would remain the main element limitating plant diversity but its availability would be controlled by phosphorus.

A note on the convergence of nonparametric DEA estimators for production efficiency scores

Abstract: Efficiency scores of production units are measured by their distance to an estimated production frontier. Nonparametric data envelopment analysis estimators are based on a finite sample of observed production units, and radial distances are considered.We investigate the consistency and the speed of convergence of these estimated efficiency scores ~or of the radial distances! in the very general setup of a multioutput and multi-input case. It is shown that the speed of convergence relies on the smoothness of the unknown frontier and on the number of inputs and outputs. Furthermore, one has to distinguish between the output- and the input-oriented cases.

Prebiotics and synbiotics: concepts and nutritional properties.

Abstract: The main role of diet is to provide enough nutrients to meet the requirements of a balanced diet, while giving the consumer a feeling of satisfaction and well-being. The most recent knowledge in bioscience supports the hypothesis that diet also controls and modulates various functions in the body, and, in doing so, contributes to the state of good health necessary to reduce the risk of some diseases. It is such an hypothesis which is at the origin both of the concept of 'functional food' and the development of a new scientific discipline of 'functional food science'. In the context of this paper the potential 'functional foods' to be discussed are the prebiotics and the synbiotics. The prebiotics developed so far are the non-digestible oligosaccharides and especially the non-digestible fructans among which chicory fructans play a major role. The chicory fructans are beta (2-1) fructo-oligosaccharides classified as natural food ingredients. They positively affect various physiological functions in such a way that they are already or may, in the future, be classified as functional food ingredients for which claims of functional effects or of disease risk reduction might become authorized. They are classified as prebiotic and have been shown to induce an increase in the number of bifidobacteria in human faecal flora. As part of a synbiotic-type product, they are already bifidogenic at a dose of 2.75 g/d and the effect lasts for at least 7 weeks. The other potential functional effects are on the bioavailability of minerals, but also, and more systemically, on the metabolism of lipids. Potential health benefits may concern reduction of the risk of intestinal infectious diseases, cardiovascular disease, non-insulin-dependent diabetes, obesity, osteoporosis and cancer. However, except for the prebiotic effect, and tentatively the improvement of calcium bioavailability, the evidence to support such effects is still missing in humans though hypotheses already exist to justify nutrition studies.

Surface Reconstructions and Dimensional Changes in Single-Walled Carbon Nanotubes

Abstract: Dimensional stability is crucial to possible applications of single-walled nanotubes, as their properties are linked to size and topology. We observe nanotubes responding to uniform atom loss, through surface reconstruction and drastic dimensional changes. Experiments using electron irradiation evidence nanotube diameters shrinking from similar to 1.4 to 0.4 nm. Molecular dynamics simulations show that surface reconstruction and size reduction occur through dangling bond saturation, forming nonhexagonal rings and 5-7 defects in the lattice. Nonuniform atom removal results in inhomogeneous tube deformations and local necking, and formation of linear atomic carbon chains in the nanotube body.

Mechanics and energetics of human locomotion on sand

Abstract: Moving about in nature often involves walking or running on a soft yielding substratum such as sand, which has a profound effect on the mechanics and energetics of locomotion. Force platform and cinematographic analyses were used to determine the mechanical work performed by human subjects during walking and running on sand and on a hard surface. Oxygen consumption was used to determine the energetic cost of walking and running under the same conditions. Walking on sand requires 1.6-2.5 times more mechanical work than does walking on a hard surface at the same speed. In contrast, running on sand requires only 1.15 times more mechanical work than does running on a hard surface at the same speed. Walking on sand requires 2.1-2.7 times more energy expenditure than does walking on a hard surface at the same speed; while running on sand requires 1.6 times more energy expenditure than does running on a hard surface. The increase in energy cost is due primarily to two effects: the mechanical work done on the sand, and a decrease in the efficiency of positive work done by the muscles and tendons.

Patent Breadth, Patent Life, and the Pace of Technological Progress

Abstract: In active investment climates where firms sequentially improve each other's products, a patent can terminate either because it expires or because a non-infringing innovation displaces its product in the market. We define the length of time until one of these happens as the effective patent life, and show how it depends on patent breadth. We distinguish lagging breadth, which protects against imitation, from leading breadth, which protects against new improved products. We compare two types of patent policy with leading breadth: (1) patents are finite but very broad, so that the effective life of a patent coincides with its statutory life, and (2) patents are long but narrow, so that the effective life of a patent ends when a better product replaces it. The former policy improves the diffusion of new products, but the latter has lower R&D costs.

A Practical Implementation of the Timing Attack

Abstract: When the running time of a cryptographic algorithm is non-constant, timing measurements can leak information about the secret key. This idea, first publicly introduced by Kocher, is developed here to attack an earlier version of the CASCADE smart card. We propose several improvements on Kocher’s ideas, leading to a practical implementation that is able to break a 512-bit key in few hours, provided we are able to collect 300000 timing measurements (128-bit keys can be recovered in few seconds using a personal computer and less than 10000 samples). We therefore show that the timing attack represents an important threat against cryptosystems, which must be very seriously taken into account.

YopT, a new Yersinia Yop effector protein, affects the cytoskeleton of host cells

Abstract: Extracellular Yersinia disarm the immune system of their host by injecting effector Yop proteins into the cytosol of target cells. Five effectors have been described: YopE, YopH, YpkA/YopO, YopP and YopM. Delivery of these effectors by Yersinia adhering at the cell surface requires other Yops (translocators) including YopB. Effector and translocator Yops are secreted by the type III Ysc secretion apparatus, and some Yops also need a specific cytosolic chaperone, called Syc. In this paper, we describe a new Yop, which we have called YopT (35.5kDa). Its secretion required an intact Ysc apparatus and SycT (15.0kDa, pl4.4), a new chaperone resembling SycE. Infection of macrophages with a Yersinia, producing a hybrid YopT-adenylate cyclase, led to the accumulation of intracellular cAMP, indicating that YopT is delivered into the cytosol of eukaryotic cells. Infection of HeLa cells with a mutant strain devoid of the five known Yop effectors (deltaHOPEM strain) but producing YopT resulted in the alteration of the cell cytoskeleton and the disruption of the actin filament structure. This cytotoxic effect was caused by YopT and dependent on YopB. YopT is thus a new effector Yop and a new bacterial toxin affecting the cytoskeleton of eukaryotic cells.

Hematopoietic Stem-Cell Transplantation in Globoid-Cell Leukodystrophy

Abstract: BACKGROUND: Globoid-cell leukodystrophy is caused by a deficiency of galactocerebrosidase, which results in progressive central nervous system deterioration. We investigated whether allogeneic hematopoietic stem-cell transplantation can provide a source of leukocyte galactocerebrosidase and thereby prevent the decline of central nervous system function in patients with the disease. METHODS: Five children with globoid-cell leukodystrophy (one with the infantile type and four with late-onset disease) were treated with allogeneic hematopoietic stem-cell transplantation. Measurement of leukocyte galactocerebrosidase levels, neurologic examinations, neuropsychological tests, magnetic resonance imaging of the central nervous system, cerebrospinal fluid protein assays, and neurophysiologic measurements were performed before and after transplantation, with follow-up ranging from one to nine years. RESULTS: Engraftment of donor-derived hematopoietic cells occurred in all patients and was followed by restoration of normal leukocyte galactocerebrosidase levels. In the four patients with late-onset disease, the central nervous system deterioration was reversed, and in the patient with the infantile form of the disease, signs and symptoms have not appeared. Magnetic resonance imaging showed a decrease in signal intensity in the three patients with late-onset disease who were assessed both before and after transplantation. Abnormalities in cerebrospinal fluid total protein levels were corrected in three patients with late-onset disease and substantially reduced in the patient with the infantile form. CONCLUSIONS:Central nervous system manifestations of globoid-cell leukodystrophy can be reversed by allogeneic hematopoietic stem-cell transplantation.

LAGE-1, a new gene with tumor specificity.

Abstract: Representational difference analysis was used to identify genes that are expressed in a human melanoma cell line and not in normal skin. A cDNA clone that appeared to be specific for tumors was obtained and the corresponding gene was sequenced. This new gene was named LAGE-I. Using a LAGE-I probe to screen a cDNA library from the same melanoma cell line, we identified a closely related gene, which proved to be identical to NY-ESO-I, a gene recently reported to code for an antigen recognized by autologous antibodies in an esophageal squamous cell carcinoma. Gene LAGE-I maps to Xq28. It comprises 3 exons. Alternative splicing produces 2 major transcripts encoding polypeptides of 210 and 180 residues, respectively. Expression of LAGE-I was observed in 25-50% of tumor samples of melanomas, non-small-cell lung carcinomas, bladder, prostate and head and neck cancers. The only normal tissue that expressed the gene was testis. As for MAGE-AI, expression of LAGE-I is induced by deoxy-azacytidine in lymphoblastoid cells, suggesting that tumoral expression is due to demethylation. The expression of LAGE-I is strongly correlated with that of NY-ESO-I. It is also clearly correlated with the expression of MAGE genes.

Sustainability Indicators at the Firm Level

Abstract: Summary After reviewing recent attempts to develop sustainability indicators, this article shows how the principles of productive efficiency can be used to elaborate such indicators at the firm level. The theory of productive efficiency is somewhat expanded to incorporate fundamental issues of sustainable development: environment, equity, and futurtty. Efficiency, in the expanded notion of productive efficiency, is viewed as a necessary condition for sustainability. Working with aggregate performance indicators, it is important not to lose track of the relevant basic information. Therefore, instead of elaborating one unique indicator; we propose to implement several kinds of indicators, each of which stresses one particular focus (e.g., environmental vs. social concems). The definition of sustainable development indicators is illustrated with reference to a small data set of U.S. fossil fuel-fired electric utilities. In a sustainabiltty perspective, two important aspects are stressed, namely, the use of nonmewable resources and the inclusion of employment as a variable to maximize rather than an input to minimize. The article ends with a discussion of the significance of, and limits to, the proposed indicators.