Showing papers by "University College Cork published in 2000"

Biochemical pathways for the production of flavour compounds in cheeses during ripening: A review

Abstract: The principal pathways for the formation of flavour compounds in cheese (glycolysis, lipolysis and proteolysis) are reviewed. Depending on variety, microflora and ripening conditions, lac- tate may be metabolized by a number of pathways to various compounds which contribute to cheese flavour or off-flavours. Citrate metabolism by citrate-positive lactococci or Leuconostoc spp. is important in certain varieties (e.g., Dutch cheeses). Lipolysis results directly in the formation of flavour compounds by liberating free fatty acids (FFA). FFA may also be metabolized to alkan-2-ones and fatty acid lactones. Proteolysis of the caseins to a range of small- and intermediate-sized peptides and free amino acids (FAA) probably only contributes to the background flavour of most cheese varieties, but FAA are important precursors for a range of poorly-understood catabolic reactions which produce volatile compounds essential for flavour.

Locally compact quantum groups

Abstract: These lecture notes are intended as an introduction to the theory of locally compact quantum groups that are studied in the framework of operator algebras, i.e. C*-algebras and von Neumann algebras. The presentation revolves around the definition of a locally compact quantum group as given in [KuV00a] and [KuV03].

Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L. salivarius strains during heat treatment and spray drying.

Abstract: Spray drying of skim milk was evaluated as a means of preserving Lactobacillus paracasei NFBC 338 and Lactobacillus salivarius UCC 118, which are human-derived strains with probiotic potential. Our initial experiments revealed that NFBC 338 is considerably more heat resistant in 20% (wt/vol) skim milk than UCC 118 is; the comparable decimal reduction times were 11.1 and 1.1 min, respectively, at 59 degrees C. An air outlet temperature of 80 to 85 degrees C was optimal for spray drying; these conditions resulted in powders with moisture contents of 4.1 to 4.2% and viable counts of 3.2 x 10(9) CFU/g for NFBC 338 and 5.2 x 10(7) CFU/g for UCC 118. Thus, L. paracasei NFBC 338 survived better than L. salivarius UCC 118 during spray drying; similar results were obtained when we used confocal scanning laser microscopy and LIVE/DEAD BacLight viability staining. In addition, confocal scanning laser microscopy revealed that the probiotic lactobacilli were located primarily in the powder particles. Although both spray-dried cultures appeared to be stressed, as shown by increased sensitivity to NaCl, bacteriocin production by UCC 118 was not affected by the process, nor was the activity of the bacteriocin peptide. The level of survival of NFBC 338 remained constant at approximately 1 x 10(9) CFU/g during 2 months of powder storage at 4 degrees C, while a decline in the level of survival of approximately 1 log (from 7.2 x 10(7) to 9.5 x 10(6) CFU/g) was observed for UCC 118 stored under the same conditions. However, survival of both Lactobacillus strains during powder storage was inversely related to the storage temperature. Our data demonstrate that spray drying may be a cost-effective way to produce large quantities of some probiotic cultures.

Ryanodine receptor mutations in malignant hyperthermia and central core disease.

Abstract: Malignant hyperthermia (MH) is a pharmacogenetic disorder of skeletal muscle that manifests in response to anesthetic triggering agents. Central core disease (CCD) is a myopathy closely associated with MH. Both MH and CCD are primarily disorders of calcium regulation in skeletal muscle. The ryanodine receptor (RYR1) gene encodes the key channel which mediates calcium release in skeletal muscle during excitation-contraction coupling, and mutations in this gene are considered to account for susceptibility to MH (MHS) in more than 50% of cases and in the majority of CCD cases. To date, 22 missense mutations in the 15,117 bp coding region of the RYR1 cDNA have been found to segregate with the MHS trait, while a much smaller number of these mutations is associated with CCD. The majority of RYR1 mutations appear to be clustered in the N-terminal amino acid residues 35-614 (MH/CCD region 1) and the centrally located residues 2163-2458 (MH/CCD region 2). The only mutation identified outside of these regions to date is a single mutation associated with a severe form of CCD in the highly conserved C-terminus of the gene. All of the RYR1 mutations result in amino acid substitutions in the myoplasmic portion of the protein, with the exception of the mutation in the C-terminus, which resides in the lumenal/transmembrane region. Functional analysis shows that MHS and CCD mutations produce RYR1 abnormalities that alter the channel kinetics for calcium inactivation and make the channel hyper- and hyposensitive to activating and inactivating ligands, respectively. The likely deciding factors in determining whether a particular RYR1 mutation results in MHS alone or MHS and CCD are: sensitivity of the RYR1 mutant proteins to agonists; the level of abnormal channel-gating caused by the mutation; the consequential decrease in the size of the releasable calcium store and increase in resting concentration of calcium; and the level of compensation achieved by the muscle with respect to maintaining calcium homeostasis. From a diagnostic point of view, the ultimate goal of development of a simple non-invasive test for routine diagnosis of MHS remains elusive. Attainment of this goal will require further detailed molecular genetic investigations aimed at solving heterogeneity and discordance issues in MHS; new initiatives aimed at identifying modulating factors that influence the penetrance of clinical MH in MHS individuals; and detailed studies aimed at describing the full epidemiological picture of in vitro responses of muscle to agents used in diagnosis of MH susceptibility.

A framework analysis of the open source software development paradigm

Abstract: Open Source Software (OSS) has become the subject of much commercial interest of late. Certainly, OSS seems to hold much promise in addressing the core issues of the software crisis, namely that of software taking too long to develop, exceeding its budget, and not working very well. Indeed, there have been several examples of significant OSS success stories—the Linux operating system, the Apache web server, the BIND domain name resolution utility, to name but a few. However, little by way of rigorous academic research on OSS has been conducted to date. In this study, a framework was derived from two previous frameworks which have been very influential in the IS field, namely that of Zachman’s IS architecture (ISA) and Checkland’s CATWOE framework from Soft Systems Methodology (SSM). The resulting framework is used to analyze the OSS approach in detail. The potential future of OSS research is also discussed.

Chaotic Electronics in Telecommunications

Abstract: Introduction CHAOS AT CODE LEVEL Introduction to DS-CDMA Chaos-Based Asynchronous DS-CDMA systems. Sequences of I.I.D Random Variables CHAOS AT SIGNAL LEVEL Introduction to Schemes for Communicating with Chaotic Signals Noise-Filtering in Chaotic Communications Chaotic Modulation Schemes Synthesis and Design CHAOS AT HARDWARE LEVEL Introduction Noise Generation in Silicon Robustness of Chaos in Analog Implementations.

Genetics and pathogenesis of malignant hyperthermia.

Abstract: Malignant hyperthermia (MH) is a potentially life-threatening event in response to anesthetic triggering agents, with symptoms of sustained uncontrolled skeletal muscle calcium homeostasis resulting in organ and systemic failure. Susceptibility to MH, an autosomal dominant trait, may be associated with congenital myopathies, but in the majority of the cases, no clinical signs of disease are visible outside of anesthesia. For diagnosis, a functional test on skeletal muscle biopsy, the in vitro contracture test (IVCT), is performed. Over 50% of the families show linkage of the IVCT phenotype to the gene encoding the skeletal muscle ryanodine receptor and over 20 mutations therein have been described. At least five other loci have been defined implicating greater genetic heterogeneity than previously assumed, but so far only one further gene encoding the main subunit of the voltage-gated dihydropyridine receptor has a confirmed role in MH. As a result of extensive research on the mechanisms of excitation-contraction coupling and recent functional characterization of several disease-causing mutations in heterologous expression systems, much is known today about the molecular etiology of MH.

New Evidence for Black Hole Event Horizons from Chandra

Abstract: Previously we claimed that Black Hole X-ray Novae (BHXN) in quiescence are much less luminous than equivalent Neutron Star X-ray Novae (NSXN). This claim was based on the quiescent detection of a single short period BHXN (A0620-00, P(orb)=7.8 hrs) and two longer period BHXN (GRO J1655-40, P(orb)=62.9 hrs; V404 Cyg, P(orb)=155.3 hrs), along with sensitive upper limits. We announce the detection of two more short period BHXN (GRO J0422+32, P(orb)=5.1 hrs; GS 2000+25, P(orb)=8.3 hrs), an upper limit for a third which is improved by two orders of magnitude (4U 1543-47, P(orb)=27.0 hrs) and a new, much lower quiescent measurement of GRO J1655-40. Taken together, these new Chandra measurements confirm that the quiescent X-ray luminosities of BHXN are significantly lower than those of NSXN. We argue that this provides strong evidence for the existence of event horizons in BHXN.

Heat shock proteins--modulators of apoptosis in tumour cells.

Abstract: Apoptosis is a genetically programmed, physiological method of cell destruction. A variety of genes are now recognised as positive or negative regulators of this process. Expression of inducible heat shock proteins (hsp) is known to correlate with increased resistance to apoptosis induced by a range of diverse cytotoxic agents and has been implicated in chemotherapeutic resistance of tumours and carcinogenesis. Intensive research on apoptosis over the past number of years has provided significant insights into the mechanisms and molecular events that occur during this process. The modulatory effects of hsps on apoptosis are well documented, however, the mechanisms of hsp-mediated protection against apoptosis remain to be fully defined, although several hypotheses have been proposed. Elucidation of these mechanisms should reveal novel targets for manipulating the sensitivity of leukaemic cells to therapy. This review aims to explain the currently understood process of apoptosis and the effects of hsps on this process. Several proposed mechanisms for hsp protection against apoptosis and the therapeutic implications of hsps in leukaemia are also discussed.

Lessons from Enterprise Resource Planning Implementations in Ireland – Towards Smaller and Shorter ERP Projects:

Abstract: The enterprise resource planning (ERP) software market has been growing at a very fast pace over the last few years and has been predicted to keep growing rapidly in the long term. This has led to an abundance of media reports on the subject of ERP and to managers wondering whether their companies should implement ERP systems. In order to separate the reality of the ERP phenomenon from the hype that surrounds it, we studied 14 ERP implementation projects in Irish organizations and focused on the key relationships between organizations which attempt to implement ERP systems and their implementing partners. We found that the ERP implementations that are going on in Ireland at the moment are different to the projects that have been reported elsewhere in two key respects. Firstly, the organizations interested in ERP software are, on average, far smaller than the case studies reported in the literature and the majority of the cases we reviewed were small and medium enterprises (SMEs). Secondly, the durations of implementation were far shorter than reported elsewhere. These results are not surprising if one considers the smaller average size of Irish organizations, but they indicate that the ERP movement is truly ready for an extension towards the SME market. They also indicate that the duration of the implementation of ERP software may be related to the size and complexity of the client organization and that SMEs can expect to have an easier time implementing ERPs than the current literature suggests. We also found that software implementers play a key role, not only in technical terms, but also in managerial and political terms, because they can help their clients in correcting their expectations and perceptions of ERP systems and ERP implementations.

Relational frame theory and Skinner’s Verbal Behavior : A possible synthesis

Abstract: The current article suggests a possible synthesis of Skinner's (1957) treatment of verbal behavior with the more recent behavioral interpretation of language known as relational frame theory. The rationale for attempting to combine these two approaches is first outlined. Subsequently, each of the verbal operants described by Skinner is examined and subjected to a relational frame analysis. In each case, two types of operants are identified; one based on direct contingencies of reinforcement and the other based on arbitrarily applicable relational responding. The latter operants are labeled verbal because they can be distinguished from other forms of social behavior, and they appear to possess the symbolic or referential qualities often ascribed to human language. By applying relational frame theory to Skinner's verbal operants, we aim to contribute towards the development of a modern behavior-analytic research agenda in human language and cognition.

A DNA diagnostic biosensor: development, characterisation and performance

Abstract: The aim of this work is to develop a sensor for specific DNA sequences, using non-complex synthetic single-stranded oligonucleotides as a model system. A capacitance-based sensor for the direct detection of DNA sequences is described. Hybridisation of analyte DNA with immobilised DNA on the silicon surface induces charge effects, altering the dielectric properties of the biolayer, and can be detected by the associated change in the measured capacitance. DNA has been immobilised on a silicon electrode either by passive adsorption or covalent coupling via 4-aminobutyldimethylmethoxysilane (4-ABDMMS). The work presented here introduces a colourimetric immunodetection technique for the evaluation of the immobilisation process and describes the electrical characterisation and performance of three silicon-based sequence-specific DNA sensors. These sensors consisted of a standard electrolyte-insulator-semiconductor (EIS) structure with covalently bound probe DNA, a mechanically degraded structure with passively adsorbed probe DNA and a mechanically degraded structure with covalently bound probe DNA. The last device had an improved signal to noise ratio and was, therefore, used to construct a standard curve, revealing a detection Limit of 100 pmol DNA. On addition of analyte DNA, then was a decrease in measured capacitance. This response was fast, specific and required no addition of mediators to enhance or amplify the signal. This device can be optimised for the detection of complex sequences.

Growth/Differentiation Factor-15/Macrophage Inhibitory Cytokine-1 Is a Novel Trophic Factor for Midbrain Dopaminergic Neurons In Vivo

Abstract: Transforming growth factor-betas (TGF-betas) constitute an expanding family of multifunctional cytokines with prominent roles in development, cell proliferation, differentiation, and repair. We have cloned, expressed, and raised antibodies against a distant member of the TGF-betas, growth/differentiation factor-15 (GDF-15). GDF-15 is identical to macrophage inhibitory cytokine-1 (MIC-1). GDF-15/MIC-1 mRNA and protein are widely distributed in the developing and adult CNS and peripheral nervous systems, including choroid plexus and CSF. GDF-15/MIC-1 is a potent survival promoting and protective factor for cultured and iron-intoxicated dopaminergic (DAergic) neurons cultured from the embryonic rat midbrain floor. The trophic effect of GDF-15/MIC-1 was not accompanied by an increase in cell proliferation and astroglial maturation, suggesting that GDF-15/MIC-1 probably acts directly on neurons. GDF-15/MIC-1 also protects 6-hydroxydopamine (6-OHDA)-lesioned nigrostriatal DAergic neurons in vivo. Unilateral injections of GDF-15/MIC-1 into the medial forebrain bundle just above the substantia nigra (SN) and into the left ventricle (20 microgram each) immediately before a 6-OHDA injection (8 microgram) prevented 6-OHDA-induced rotational behavior and significantly reduced losses of DAergic neurons in the SN. This protection was evident for at least 1 month. Administration of 5 microgram of GDF-15/MIC-1 in the same paradigm also provided significant neuroprotection. GDF-15/MIC-1 also promoted the serotonergic phenotype of cultured raphe neurons but did not support survival of rat motoneurons. Thus, GDF-15/MIC-1 is a novel neurotrophic factor with prominent effects on DAergic and serotonergic neurons. GDF-15/MIC-1 may therefore have a potential for the treatment of Parkinson's disease and disorders of the serotonergic system.

Reflexive Historical Sociology

Abstract: This paper attempts to reassess the standard sociological canon and sketch the outlines of a new approach by bringing together a series of thinkers whose works so far have remained disconnected. Introducing a distinction between classics and background figures who were crucial sources of inspiration, it shifts emphasis to the late, reflexive works of Durkheim and Weber. These are sources for two types of reflexive sociology: historical and anthropological. The main background figures of reflexive historical sociology are Marx, Kierkegaard, Nietzsche and Freud, while its protagonists include Foucault, Elias, Voegelin, Borkenau, Mumford, Aries and Koselleck. A short introduction will be given to the four main fields of interest within the approach: the reconstructive histories of subjectivity, of forms of thought, of forms of knowledge, and of closed space and regulated time.

Use of Hydrostatic Pressure for Inactivation of Microbial Contaminants in Cheese

Abstract: The objective of this study was to determine the effect of high pressure (HP) on the inactivation of microbial contaminants in Cheddar cheese (Escherichia coli K-12, Staphylococcus aureus ATCC 6538, and Penicillium roqueforti IMI 297987). Initially, cheese slurries inoculated with E. coli, S. aureus, and P. roqueforti were used as a convenient means to define the effects of a range of pressures and temperatures on the viability of these microorganisms. Cheese slurries were subjected to pressures of 50 to 800 MPa for 20 min at temperatures of 10, 20, and 30 degrees C. At 400 MPa, the viability of P. roqueforti in cheese slurry decreased by >2-log-unit cycles at 10 degrees C and by 6-log-unit cycles at temperatures of 20 and 30 degrees C. S. aureus and E. coli were not detected after HP treatments in cheese slurry of >600 MPa at 20 degrees C and >400 MPa at 30 degrees C, respectively. In addition to cell death, the presence of sublethally injured cells in HP-treated slurries was demonstrated by differential plating using nonselective agar incorporating salt or glucose. Kinetic experiments of HP inactivation demonstrated that increasing the pressure from 300 to 400 MPa resulted in a higher degree of inactivation than increasing the pressurization time from 0 to 60 min, indicating a greater antimicrobial impact of pressure. Selected conditions were subsequently tested on Cheddar cheese by adding the isolates to cheese milk and pressure treating the resultant cheeses at 100 to 500 MPa for 20 min at 20 degrees C. The relative sensitivities of the isolates to HP in Cheddar cheese were similar to those observed in the cheese slurry, i.e., P. roqueforti was more sensitive than E. coli, which was more sensitive than S. aureus. The organisms were more sensitive to pressure in cheese than slurry, especially with E. coli. On comparison of the sensitivities of the microorganisms in a pH 5.3 phosphate buffer, cheese slurry, and Cheddar cheese, greatest sensitivity to HP was shown in the pH 5.3 phosphate buffer by S. aureus and P. roqueforti while greatest sensitivity to HP by E. coli was exhibited in Cheddar cheese. Therefore, the medium in which the microorganisms are treated is an important determinant of the level of inactivation observed.

Oxidative stress induces caspase-independent retinal apoptosis in vitro.

Abstract: Apoptosis is the mode of cell death in retinitis pigmentosa (RP), a heterogeneous group of retinal degenerations. The activation of the caspase proteases forms a pivotal step in the initiation and execution phase of apoptosis in many cells. Inhibition of caspases has been reported to prevent apoptosis in many model systems. However, we demonstrate the absence of caspase activation during retinal cell apoptosis in vitro which involves phosphatidylserine (PS) externalisation, DNA nicking and cell shrinkage. In addition, zVAD-fmk, DEVD-CHO and BD-fmk, inhibitors of the caspases, were unable to alter the characteristics or kinetics of apoptosis, implying that retinal cell death in vitro follows a caspase-independent pathway. We have previously demonstrated the ability of reactive oxygen species (ROS) to act as mediators of retinal cell apoptosis in vitro as well as the ability of antioxidants to prevent retinal cell apoptosis. Here we demonstrate the oxidative inactivation of caspases in this model of retinal apoptosis and provide evidence for an oxidative stress driven cell death pathway that does not involve caspase activity and which retains key features of apoptotic cell death. Furthermore, our data indicates that apoptotic events such as PS exposure, DNA nicking and cell shrinkage may occur independently of caspase activity.

Systems development methodologies: the problem of tenses

Abstract: Presents two fundamental arguments. First, it is proposed that most of the currently available systems development methodologies are founded on concepts which emerged in the period from about 1967 to 1977. This argument is presented through the use of literature references. The second argument is that the profile of the development environment now faced in organizations is very different from that which prevailed in the period 1967 to 1977. This is illustrated through original empirical research which supports this argument, and by contrasting these findings with those of previous studies in the literature. It is therefore argued that there is a need to update “tenses” by deriving new methodological canons more appropriate to the needs of the current development environment. Some suggestions for new methodological canons appropriate to the current development environment are provided.