Institution
University of Adelaide
Education•Adelaide, South Australia, Australia•
About: University of Adelaide is a education organization based out in Adelaide, South Australia, Australia. It is known for research contribution in the topics: Population & Poison control. The organization has 27251 authors who have published 79167 publications receiving 2671128 citations. The organization is also known as: The University of Adelaide & Adelaide University.
Topics: Population, Poison control, Pregnancy, Health care, Mental health
Papers published on a yearly basis
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TL;DR: Time-lapse video cinematography is shown to be an excellent tool for studying fertilization and early embryo development, and it was found that good quality embryos arose from oocytes that had more uniform timing from injection to pronuclear abuttal and tended to have a longer cytoplasmic wave.
Abstract: In this study, we have used time-lapse video cinematography to study fertilization in 50 human oocytes that had undergone intracytoplasmic sperm injection (ICSI). Time-lapse recording commenced shortly after ICSI and proceeded for 17-20 h. Oocytes were cultured in an environmental chamber which was maintained under standard culture conditions. Overall, 38 oocytes (76%) were fertilized normally, and the fertilization rate and embryo quality were not significantly different from 487 sibling oocytes cultured in a conventional incubator. Normal fertilization followed a defined course of events, although the timing of these events varied markedly between oocytes. In 35 of the 38 fertilized oocytes (92%), there were circular waves of granulation within the ooplasm which had a periodicity of 20-53 min. The sperm head decondensed during this granulation phase. The second polar body was then extruded, and this was followed by the central formation of the male pronucleus. The female pronucleus formed in the cytoplasm adjacent to the second polar body at the same time as, or slightly after, the male pronucleus, and was subsequently drawn towards the male pronucleus until the two abutted. Both pronuclei then increased in size, the nucleoli moved around within the pronuclei and some nucleoli coalesced. During pronuclear growth, the organelles contracted from the cortex towards the centre of the oocyte, leaving a clear cortical zone. The oocyte decreased in diameter from 112 to 106 microm (P < 0.0001) during the course of the observation period. The female pronucleus was significantly smaller in diameter than the male pronucleus (24.1 and 22.4 microm respectively, P = 0.008) and contained fewer nucleoli (4.2 and 7.0 respectively, P < 0.0001). After time-lapse recording, oocytes were cultured for 48 h prior to embryo transfer or cryopreservation. Embryo quality was related to fertilization events and periodicity of the cytoplasmic wave, and it was found that good quality embryos arose from oocytes that had more uniform timing from injection to pronuclear abuttal and tended to have a longer cytoplasmic wave. In conclusion, we have shown that time-lapse video cinematography is an excellent tool for studying fertilization and early embryo development, and have demonstrated that human fertilization comprises numerous complex dynamic events.
398 citations
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TL;DR: This estimate of global losses due to N. caninum, with the identification of clear target markets (countries, as well as cattle industries), should provide an incentive to develop treatment options and/or vaccines.
398 citations
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TL;DR: Climate change is likely to, both by itself and in synergy with other stressors, impose change to southern Australian coastal species, including important habitat-forming algae and the associated ecological functioning of temperate coasts, which provides an attractive tool for building resilience in temperate systems.
397 citations
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TL;DR: Since oxygen delivered by a nasal cannula provides no additional symptomatic benefit for relief of refractory dyspnoea in patients with life-limiting illness compared with room air, less burdensome strategies should be considered after brief assessment of the effect of oxygen therapy on the individual patient.
397 citations
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TL;DR: Evidence is presented suggesting that the phenotype of such a deletion of Aspergillus nidulans is leaky lethality allowing limited germination of the spore but not colony formation, and thus either the gene product may have an activator activity as well as a repressor function or some residual repression function may be required for full viability.
Abstract: The complete nucleotide sequence derived from a genomic clone and two cDNA clones of the creA gene of Aspergillus nidulans is presented. The gene contains no introns. The derived polypeptide of 415 amino acids contains two zinc fingers of the C2H2 class, frequent S(T)PXX motifs, and an alanine-rich region indicative of a DNA-binding repressor protein. The amino acid sequence of the zinc finger region has 84% similarity to the zinc finger region of Mig1, a protein involved in carbon catabolite repression in yeast cells, and it is related both to the mammalian Egr1 and Egr2 proteins and to the Wilms' tumor protein. A deletion removing the creA gene was obtained, by using in vitro techniques, in both a heterokaryon and a diploid strain but was unobtainable in a pure haploid condition. Evidence is presented suggesting that the phenotype of such a deletion, when not complemented by another creA allele, is leaky lethality allowing limited germination of the spore but not colony formation. This phenotype is far more extreme than that of any of the in vivo-generated mutations, and thus either the gene product may have an activator activity as well as a repressor function or some residual repressor function may be required for full viability.
397 citations
Authors
Showing all 27579 results
Name | H-index | Papers | Citations |
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Martin White | 196 | 2038 | 232387 |
Nicholas G. Martin | 192 | 1770 | 161952 |
David W. Johnson | 160 | 2714 | 140778 |
Nicholas J. Talley | 158 | 1571 | 90197 |
Mark E. Cooper | 158 | 1463 | 124887 |
Xiang Zhang | 154 | 1733 | 117576 |
John E. Morley | 154 | 1377 | 97021 |
Howard I. Scher | 151 | 944 | 101737 |
Christopher M. Dobson | 150 | 1008 | 105475 |
A. Artamonov | 150 | 1858 | 119791 |
Timothy P. Hughes | 145 | 831 | 91357 |
Christopher Hill | 144 | 1562 | 128098 |
Shi-Zhang Qiao | 142 | 523 | 80888 |
Paul Jackson | 141 | 1372 | 93464 |
H. A. Neal | 141 | 1903 | 115480 |