Showing papers by "University of Amsterdam published in 1992"

A Rank-Invariant Method of Linear and Polynomial Regression Analysis

Abstract: Regression analysis is usually carried out under the hypothesis that one of the variables is normally distributed with constant variance, its mean being a function of the other variables. This assumption is not always satisfied, and in most cases difficult to ascertain.

Incidence, Severity, Aetiology and Prevention of Sports Injuries A Review of Concepts

Abstract: Notwithstanding the healthy influence of sporting activities on risk factors, in particular those of cardiovascular disease, it is becoming increasingly apparent that sports can present a danger to health in the form of sports injuries. The extent of the sports injury problem calls for preventative action based on the results of epidemiological research. For the interpretation of these facts uniform definitions are needed and limitations of research designs should be known. Measures to prevent sports injuries form part of what is called the 'sequence of prevention'. Firstly the extent of the sports injury problem must be identified and described. Secondly the factors and mechanisms which play a part in the occurrence of sports injuries have to be identified. The third step is to introduce measures that are likely to reduce the future risk and/or severity of sports injuries. This measure should be based on the aetiological factors and the mechanism as identified in the second step. Finally the effect of the measures must be evaluated by repeating the first step. In this review some aspects of the first and second step of the sequence of prevention are discussed. The extent of the sports injury problem is often described by injury incidence and by indicators of the severity of sports injuries. Sports injury incidence should preferably be expressed as the number of sports injuries per exposure time (e.g. per 1000 hours of sports participation) in order to facilitate the comparability of research results. However, one should realise that the outcome of research applying this definition of sports injury incidence is highly dependent on the definitions of 'sports injury' and 'sports participation'. The outcome of such research also depends on the applied research design and research methodology. The incidence of sports injuries depends on: the method used to count injuries (e.g. prospective vs retrospective); the method used to establish the population at risk; and on the representativeness of the sample. Severity of sports injuries can be described on the basis of 6 criteria: the nature of the sports injury; the duration and nature of treatment; sporting time lost; working time lost; permanent damage; and cost. Here also uniform definitions are important and necessary in order to enhance the comparability of research data. In the second step of the 'sequence of prevention' the aetiological factors that play a role in the occurrence of a sports injury have to be identified by epidemiological studies. Epidemiological research on the aetiology of sports injuries requires a conceptual model.(ABSTRACT TRUNCATED AT 400 WORDS)

Modern Operating Systems

Abstract: For software development professionals and computer science students, Modern Operating Systems gives a solid conceptual overview of operating system design, including detailed case studies of Unix/Linux and Windows 2000. What makes an operating system modern? According to author Andrew Tanenbaum, it is the awareness of high-demand computer applications--primarily in the areas of multimedia, parallel and distributed computing, and security. The development of faster and more advanced hardware has driven progress in software, including enhancements to the operating system. It is one thing to run an old operating system on current hardware, and another to effectively leverage current hardware to best serve modern software applications. If you don't believe it, install Windows 3.0 on a modern PC and try surfing the Internet or burning a CD. Readers familiar with Tanenbaum's previous text, Operating Systems, know the author is a great proponent of simple design and hands-on experimentation. His earlier book came bundled with the source code for an operating system called Minux, a simple variant of Unix and the platform used by Linus Torvalds to develop Linux. Although this book does not come with any source code, he illustrates many of his points with code fragments (C, usually with Unix system calls). The first half of Modern Operating Systems focuses on traditional operating systems concepts: processes, deadlocks, memory management, I/O, and file systems. There is nothing groundbreaking in these early chapters, but all topics are well covered, each including sections on current research and a set of student problems. It is enlightening to read Tanenbaum's explanations of the design decisions made by past operating systems gurus, including his view that additional research on the problem of deadlocks is impractical except for "keeping otherwise unemployed graph theorists off the streets." It is the second half of the book that differentiates itself from older operating systems texts. Here, each chapter describes an element of what constitutes a modern operating system--awareness of multimedia applications, multiple processors, computer networks, and a high level of security. The chapter on multimedia functionality focuses on such features as handling massive files and providing video-on-demand. Included in the discussion on multiprocessor platforms are clustered computers and distributed computing. Finally, the importance of security is discussed--a lively enumeration of the scores of ways operating systems can be vulnerable to attack, from password security to computer viruses and Internet worms. Included at the end of the book are case studies of two popular operating systems: Unix/Linux and Windows 2000. There is a bias toward the Unix/Linux approach, not surprising given the author's experience and academic bent, but this bias does not detract from Tanenbaum's analysis. Both operating systems are dissected, describing how each implements processes, file systems, memory management, and other operating system fundamentals. Tanenbaum's mantra is simple, accessible operating system design. Given that modern operating systems have extensive features, he is forced to reconcile physical size with simplicity. Toward this end, he makes frequent references to the Frederick Brooks classic The Mythical Man-Month for wisdom on managing large, complex software development projects. He finds both Windows 2000 and Unix/Linux guilty of being too complicated--with a particular skewering of Windows 2000 and its "mammoth Win32 API." A primary culprit is the attempt to make operating systems more "user-friendly," which Tanenbaum views as an excuse for bloated code. The solution is to have smart people, the smallest possible team, and well-defined interactions between various operating systems components. Future operating system design will benefit if the advice in this book is taken to heart. --Pete Ostenson

Biological phenotype of human immunodeficiency virus type 1 clones at different stages of infection: progression of disease is associated with a shift from monocytotropic to T-cell-tropic virus population.

Abstract: The composition of human immunodeficiency virus type 1 (HIV-1) clonal populations at different stages of infection and in different compartments was analyzed. Biological HIV-1 clones were obtained by primary isolation from patient peripheral blood mononuclear cells under limiting dilution conditions, with either blood donor peripheral blood lymphocytes or monocyte-derived macrophages (MDM) as target cells, and the biological phenotype of the clones was analyzed. In asymptomatic individuals, low frequencies of HIV-1 clones were observed. These clones were non-syncytium inducing and preferentially monocytotropic. In individuals progressing to disease, a 100-fold increase in frequencies of productively HIV-1-infected cells was observed as a result of a selective expansion of nonmonocytotropic clones. In a person progressing to AIDS within 19 months after infection, only syncytium-inducing clones were detected, shifting from MDM-tropic to non-MDM-tropic over time. From his virus donor, a patient with wasting syndrome, only syncytium-inducing clones, mostly non-MDM-tropic, were recovered. Parallel clonal analysis of HIV-1 populations in cells present in bronchoalveolar lavage fluid and peripheral blood from an AIDS patient revealed a qualitatively and quantitatively more monocytotropic virus population in the lung compartment than in peripheral blood at the same time point. These findings indicate that monocytotropic HIV-1 clones, probably generated in the tissues, are responsible for the persistence of HIV-1 infection and that progression of HIV-1 infection is associated with a selective increase of T-cell-tropic, nonmonocytotropic HIV-1 variants in peripheral blood.

Discourse and the Denial of Racism

Abstract: Within the broader framework of a research programme on the reproduction of racism in discourse and communication, the present article examines the prominent role of the denial of racism, especiall...

Programmed death of T cells in HIV-1 infection

Abstract: In human immunodeficiency virus (HIV) infection, functional defects and deletion of antigen-reactive T cells are more frequent than can be explained by direct viral infection. On culturing, both CD4+ and CD8+ T cells from asymptomatic HIV-infected individuals died as a result of programmed cell death (apoptosis). Apoptosis was enhanced by activation with CD3 antibodies. Programmed cell death, associated with impaired T cell reactivity, may thus be responsible for the deletion of reactive T cells that contributes to HIV-induced immunodeficiency.

The complete DNA sequence of yeast chromosome III.

Abstract: The entire DNA sequence of chromosome III of the yeast Saccharomyces cerevisiae has been determined. This is the first complete sequence analysis of an entire chromosome from any organism. The 315-kilobase sequence reveals 182 open reading frames for proteins longer than 100 amino acids, of which 37 correspond to known genes and 29 more show some similarity to sequences in databases. Of 55 new open reading frames analysed by gene disruption, three are essential genes; of 42 non-essential genes that were tested, 14 show some discernible effect on phenotype and the remaining 28 have no overt function.

Cultural variations in emotions: A review.

Abstract: The psychological and anthropological literature on cultural variations in emotions is reviewed The literature has been interpreted within the framework of a cognitive-process model of emotions Both cross-cultural differences and similarities were identified in each phase of the emotion process; similarities in 1 phase do not necessarily imply similarities in other phases Whether cross-cultural differences or similarities are found depends to an important degree on the level of description of the emotional phenomena Cultural differences in emotions appear to be due to differences in event types or schemas, in culture-specific appraisal propensities, in behavior repertoires, or in regulation processes Differences in taxonomies of emotion words sometimes reflect true emotion differences like those just mentioned, but they may also just result from differences in which emotion-process phase serves as the basis for categorization

Phenotype-associated sequence variation in the third variable domain of the human immunodeficiency virus type 1 gp120 molecule.

Abstract: The third variable (V3) domain has been implicated in determining the human immunodeficiency virus (HIV) phenotype, including fusion capacity and monocytotropism. In a large set of primary HIV type 1 (HIV-1) isolates, V3 sequence analysis revealed that fast-replicating, syncytium-inducing isolates contained V3 sequences with a significantly higher positive charge than those of slow-replicating, non-syncytium-inducing monocytotropic isolates. It appeared that these differences in charge could be attributed to highly variable amino acid residues located on either side of the V3 loop, midway between the cysteine residues and the central GPG motif. In non-syncytium-inducing monocytotropic isolates, these residues were negatively charged or uncharged, whereas in syncytium-inducing nonmonocytotropic isolates, either one or both were positively charged. The substitutions at these positions result in changes in the predicted secondary structure of the V3 loop. Our data suggest that two amino acid residues in the highly variable V3 domain are responsible for phenotype differences and point to conformational differences in V3 loops from phenotypically distinct HIV-1 isolates.

Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy.

Abstract: The temporal and spatial progression of DNA replication in interphase nuclei of eukaryotic cells has been investigated. Application of a recently developed technique for the immunofluorescence double staining of cell nuclei labelled first with iododeoxyuridine (IdUrd) and subsequently with chlorodeoxyuridine (CldUrd) allows the visualization of two replication patterns in the same nucleus originating from two different periods of the S-phase. We have analysed changes in the three-dimensional replication patterns during the S-phase. To record dual colour three-dimensional images of doubly stained nuclei, a confocal microscope is used. This CSLM is equipped with a specific laser/filter combination to collect both fluorescence signals (FITC and Texas Red) in a single scan, thus precluding pixel shift between the images. A method for the quantitative evaluation of the degree of overlap between DNA regions replicated in two different periods of the S-phase is applied. The results confirm the generally accepted theory that DNA is replicated coordinately in a specific temporal order during the S-phase. The replication time of a DNA domain (i.e. the time between initiation and termination of DNA replication within a domain) at the very beginning of the S-phase was known to be one hour (Nakamura et al., 1986). Our observations show that in the rest of the S-phase, the replication time of a DNA region is also about one hour. We conclude that replicon clusters located in the same region are replicated in the same relatively short period of time. After this period there is no unreplicated DNA left in this region.

Optical properties of intralipid: A phantom medium for light propagation studies

Abstract: Intralipid is an intravenous nutrient consisting of an emulsion of phospholipid micelles and water. Because Intralipid is turbid and has no strong absorption bands in the visible region of the electromagnetic spectrum, and is readily available and relatively inexpensive, it is often used as a tissue simulating phantom medium in light dosimetry experiments. In order to assist investigators requiring a controllable medium that over a finite range of wavelengths is optically equivalent to tissue, we have compiled previously published values of the optical interaction coefficients of Intralipid, most of which were measured at a wavelength of 633 nm. We have extended the measurements of the absorption and reduced scattering coefficients from 460 to 690 nm and the total attenuation coefficient from 500 to 890 nm. These measurements show that, for stock 10% Intralipid, the absorption coefficient varies from 0.015 to 0.001 cm-1 between 460 and 690 nm, the reduced scattering coefficient varies from 92 to 50 cm-1 between 460 and 690 nm, the total attenuation coefficient varies from 575 to 150 cm-1 between 500 and 890 nm, and the average cosine of scatter varies from 0.87 to 0.82 between 460 and 690 nm. With these data, we discuss the design of an optically tissue-equivalent phantom consisting of Intralipid and black India ink.

Free(z)ing Degrees of Freedom in Skill Acquisition

Abstract: This study reports an empirical investigation into Bernstein's (1967) ideas that in the early stages of the acquisition of a movement skill the coordination problem is reduced by an initial freezing out of degrees of freedom, followed later in the learning process by the release of these degrees of freedom and their incorporation into a dynamic, controllable system. “Freezing” degrees of freedom was made operational both as a rigid fixation of individual degrees of freedom and as the formation of rigid couplings between multiple degrees of freedom. Five subjects practiced slalom-like ski movements on a ski apparatus for 7 consecutive days. Results showed that at the early phases of learning, the joint angles of the lower limbs and torso displayed little movement, as expressed by their standard deviations and ranges of angular motion, whereas joint couplings were high, as shown by the relatively high cross correlations between joint angles. Over practice, angular movement significantly increased i...

Minimal requirements for the human immunodeficiency virus type 1 V3 domain to support the syncytium-inducing phenotype: analysis by single amino acid substitution.

Abstract: The third variable domain (V3) of the human immunodeficiency virus type 1 external envelope contains determinants of cell tropism, cytopathicity, and infectivity and elicits antibodies able to block infectivity in vitro and in vivo. Our study encompassed point-mutational analysis of HXB-2 viruses containing patient-derived V3 regions and expressing a non-syncytium-inducing, low-replicating phenotype in T-cell line SupT1. The mutation within V3 of a serine at position 306 into an also naturally occurring arginine (S to R) required an additional, naturally occurring mutation at position 320 (aspartate to glutamine, D to Q) or 324 (aspartate to asparagine, D to N) for full expression of the syncytium-inducing, high-replicating (SI) phenotype. The naturally occurring mutation of an aspartate into an arginine at position 320 (D to R) was sufficient for production of the SI phenotype. This study proves that introduction of a positively charged amino acid at position 306 or 320, previously shown to be strongly associated with the SI phenotype in field isolates (R.A.M. Fouchier, M. Groenink, N.A. Kootstra, M. Tersmette, H.G. Huisman, F. Miedema, and H. Schuitemaker, J. Virol. 66:3183-3187, 1992), is minimally required for production of SI viruses. In addition, naturally occurring mutations at residue 324 also modulate the virus phenotype.

Running injuries. A review of the epidemiological literature.

Abstract: Running is one of the most popular leisure sports activities. Next to its beneficial health effects, negative side effects in terms of sports injuries should also be recognised. Given the limitations of the studies it appears that for the average recreational runner, who is steadily training and who participates in a long distance run every now and then, the overall yearly incidence rate for running injuries varies between 37 and 56%. Depending on the specificity of the group of runners concerned (competitive athletes; average recreational joggers; boys and girls) and on different circumstances these rates vary. If incidence is calculated according to exposure of running time the incidence reported in the literature varies from 2.5 to 12.1 injuries per 1000 hours of running. Most running injuries are lower extremity injuries, with a predominance for the knee. About 50 to 75% of all running injuries appear to be overuse injuries due to the constant repetition of the same movement. Recurrence of running injuries is reported in 20 to 70% of the cases. From the epidemiological studies it can be concluded that running injuries lead to a reduction of training or training cessation in about 30 to 90% of all injuries, about 20 to 70% of all injuries lead to medical consultation or medical treatment and 0 to 5% result in absence from work. Aetiological factors associated with running injuries include previous injury, lack of running experience, running to compete and excessive weekly running distance. The association between running injuries and factors such as warm-up and stretching exercises, body height, malalignment, muscular imbalance, restricted range of motion, running frequency, level of performance, stability of running pattern, shoes and inshoe orthoses and running on 1 side of the road remains unclear or is backed by contradicting or scarce research findings. Significantly not associated with running injuries seem age, gender, body mass index, running hills, running on hard surfaces, participation in other sports, time of the year and time of the day. The prevention of sports injuries should focus on changes of behaviour by health education. Health education on running injuries should primarily focus on the importance of complete rehabilitation and the early recognition of symptoms of overuse, and on the provision of training guidelines.

Acenocoumarol and Heparin Compared with Acenocoumarol Alone in the Initial Treatment of Proximal-Vein Thrombosis

Abstract: Background. In most countries, heparin is used in the initial treatment of patients with deep-vein thrombosis. Well-designed studies establishing the efficacy of heparin therapy are lacking, however. Treatment with acenocoumarol alone, according to the hypothesis that high dosages of oral anticoagulants obviate the need for heparin, is considered an effective alternative in some countries. Methods. In a randomized, double-blind study we compared the efficacy and safety of continuous intravenous heparin plus acenocoumarol with the efficacy and safety of acenocoumarol alone in the initial treatment of outpatients with proximal-vein thrombosis. The principal study end point was a confirmed symptomatic extension or recurrence of venous thromboembolism during six months of follow-up. In addition, we assessed asymptomatic extension or pulmonary embolism by repeating venography and lung scanning after the first week of treatment. The incidence of major bleeding was determined during three months of foll...

Paralanguage and social perception in computer‐mediated communication

Abstract: It is widely held that computer‐mediated communication (CMC) filters out many of the social and affective cues associated with human interaction with consequent effects on communication outcomes an...

Cells in the marginal zone of the spleen.

Abstract: Publisher Summary This chapter provides an overview of the various nonlymphoid and lymphoid cell types localized in the marginal zone and the way they are arranged in the basic structure of this compartment. It also discusses the role of the marginal zone, with emphasis to the intriguing ways these cell types interact with each other in the defense against invading pathogens. The spleen can be divided into a red pulp compartment, distinguishable by the abundance of erythrocytes, and a white pulp compartment, consisting of large nodules of organized lymphoid tissue. It is in the red pulp that blood filtration takes place, whereas the white pulp is involved in the specific immunological defense against bloodborne antigens. The marginal zone is described as a layer surrounding the periarteriolar lymphocyte sheath (PALS) and B cell follicles, predominantly composed of intermediate-sized lymphocytes. The basic structure of the marginal zone consists of a meshwork of reticular cells, which are concentrically layered around the PALS and the follicular areas, with a more dispersed three-dimensional appearance toward the red pulp. The opening of a part of the arterial blood supply into the wider spaces of the marginal sinus, right at the border of the marginal zone and the white pulp, results in a blood flow with a lower resistance.

HIV-1 biological phenotype in long-term infected individuals evaluated with an MT-2 cocultivation assay.

Abstract: ObjectiveWe have previously demonstrated that detection of syncytium-inducing (SI) HIV-1 in asymptomatic seropositive individuals is associated with rapid progression to AIDS. In the present study, we sought to develop and evaluate an HIV-1 phenotyping assay for the screening of large numbers of ind

Orca: a language for parallel programming of distributed systems

Abstract: A detailed description is given of the Orca language design and the design choices are discussed. Orca is intended for applications programmers rather than systems programmers. This is reflected in its design goals to provide a simple, easy-to-use language that is type-secure and provides clean semantics. Three example parallel applications in Orca, one of which is described in detail, are discussed. One of the existing implementations, which is based on reliable broadcasting, is described. Performance measurements of this system are given for three parallel applications. The measurements show that significant speedups can be obtained for all three applications. The authors compare Orca with several related languages and systems. >

Ordered appearance of zidovudine resistance mutations during treatment of 18 human immunodeficiency virus-positive subjects.

Abstract: The order of appearance in the reverse transcriptase gene of four mutations implicated in the development of resistance to zidovudine was investigated by selective polymerase chain reaction. Serial human immunodeficiency virus isolates were studied from 18 initially asymptomatic individuals who had been treated with zidovudine for 2 years. Most subjects had similar patterns. The first mutation occurred transiently at codon 70; its disappearance was paralleled by the appearance of a mutation at codon 215. Subsequently, in some individuals, the mutation at codon 70 reappeared. During the 2 years of treatment, no mutations developed at codon 219 and only one at codon 67, suggesting that most individuals developed only partly resistant virus. This was confirmed by plaque-reduction assay. Six subjects progressed to AIDS within the 2-year study period, confirming that the development of highly resistant isolates is not required for progression in treated individuals. No clear temporal relationship was found between the development of partial resistance and progression.

On the interaction of IgG subclasses with the low affinity Fc gamma RIIa (CD32) on human monocytes, neutrophils, and platelets. Analysis of a functional polymorphism to human IgG2.

Abstract: An allotypic form of the low affinity IgG Fc receptor Fc gamma RIIa (CD32), termed low responder (LR) because of its weak reactivity with mouse (m) IgG1, interacts efficiently with human (h) IgG2. Fc gamma RIIaLR is the first known human FcR that binds this IgG subclass. In this study, we analyzed the role of Fc gamma RIIa in binding of stable hIgG-subclass dimers, and in induction of T cell mitogenesis using chimeric anti-CD3 mAb. We demonstrate that the functional polymorphism to hIgG2 is expressed on the majority of Fc gamma R-bearing peripheral blood cells: monocytes, neutrophils, and platelets. We were able to assess Fc gamma RII-mediated IgG-binding without interference of other Fc gamma R-classes, by blockade of Fc gamma RI on monocytes, and by using neutrophils of an individual deficient for the Fc gamma RIIIB gene. This study indicates as subclass specificity: hIgG3 >hIgG1,hIgG2 >> hIgG4 for Fc gamma RIIaLR and hIgG3,hIgG1 >> hIgG2 > hIgG4 for Fc gamma RIIaHR. Comparing the serum hIgG levels of individuals homozygous for the two fc gamma RIIa allotypic forms, we observed significantly lower hIgG2 serum levels in individuals expressing the hIgG2-binding LR allotypic form. This observation may implicate that Fc gamma RIIa regulates hIgG subclass production or turnover in man.