Showing papers by "University of Barcelona published in 1968"
TL;DR: It is suggested, that the activating effect of Mg2+ on the D to I transformation is due to an activation of an endogenous ATP-ase and adenylate kinase, and that diabetic leucocytes possess transferase-D phosphatase activity in contrast to normal cells.
Abstract: 1. In normal leucocytes, glycogen-transferase was only found in the glucose-6-phosphate dependent D-form. An increase in activity was observed within 30 min after glucose feeding, and is assumed to be mediated through the action of released insulin. A late secondary increase in activity occurred simultaneously with an increase in serum growth hormone. — 2. In most diabetic leucocytes, glycogen-transferase was found in both the I- (independent of glucose-6-phosphate) and D-form. The I+D activity was low in uncontrolled diabetes and increased in response to insulin treatment. The I-activity was low immediately upon isolation of the leucocytes, but increased during incubation of the enzyme, especially in the presence of Mg2+; indicating that diabetic leucocytes possess transferase-D phosphatase activity in contrast to normal cells. The D to I transformation of the enzyme was greatly increased by insulin treatment. — 3. K
m
for UDPG and K
a
for glucose-6-phosphate did not vary between normal and diabetic leucocytes. — 4. The D to I interconversion of the incubated enzyme from diabetic cells was found to depend on the concentration of ATP and ADP in the incubation medium. It is suggested, that the activating effect of Mg2+ on the D to I transformation is due to an activation of an endogenous ATP-ase and adenylate kinase.
17 citations
14 citations
TL;DR: The study presented here was undertaken in order to learn whether correlations exist between the type and place of such vascular obstructions on the one hand and the clinical and angiographic data on the other.
Abstract: * From the Neuroradiological Department, Neurosurgical Clinic (Dr. A. Ley), University of Barcelona School of Medicine, Barcelona, Spain. The variations in the degrees of gravity and the clinical course that are observed in patients with obstructive lesions of the carotid arteries or any of their intracranial branches are evidence for the wide range of efficiency of the mechanisms that compensate for such obstruction. The study presented here was undertaken in order to learn whether correlations exist between the type and place of such vascular obstructions on the one hand and the clinical and angiographic data on the other.
4 citations