University of Cagliari

About: University of Cagliari is a education organization based out in Cagliari, Italy. It is known for research contribution in the topics: Population & Dopamine. The organization has 11029 authors who have published 29046 publications receiving 771023 citations. The organization is also known as: Università degli Studi di Cagliari & Universita degli Studi di Cagliari.

First observations of the rare decays B (+) -> K (+)pi (+)pi (-)mu(+)mu (-) and B (+)-> phi K (+)mu(+)mu (-)

Abstract: First observations of the rare decays B (+) -> K (+)pi (+) pi (-) mu (+) mu (-) and B (+)-> phi K+ mu(+)mu(-) are presented using data corresponding to an integrated luminosity of 3.0 fb(-1), collected by the LHCb experiment at centre-of-mass energies of 7 and 8 TeV. The branching fractions of the decays are B(B (+) -> K (+)pi (+) pi (-) mu (+) mu (-) ) = (4.36 (-0.27) (+0.29) (stat) +/- 0.21 (syst) +/- (norm)) x 10(-7), B(B (+)-> phi K+ mu(+)mu(-)) = (0.82 (+0.19)(-0.17) (stat) (+0.10)(-0.04) (syst) +/- 0.27 (norm)) x 10(-7) where the uncertainties are statistical, systematic, and due to the uncertainty on the branching fractions of the normalisation modes. A measurement of the differential branching fraction in bins of the invariant mass squared of the dimuon system is also presented for the decay B (+) -> K (+)pi (+) pi (-) mu (+) mu (-)

Febrile urinary tract infections in young children: recommendations for the diagnosis, treatment and follow‐up

Abstract: We report the recommendations for the diagnosis, treatment, imaging evaluation and use of antibiotic prophylaxis in children with the first febrile urinary tract infection, aged 2 months to 3 years. They were prepared by a working group of the Italian Society of Pediatric Nephrology after careful review of the available literature and a consensus decision, when clear evidence was not available. Conclusion: These recommendations are endorsed by the Italian Society of Pediatric Nephrology. They can also be a tool of comparison with other existing guidelines in issues in which much controversy still exists.

Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives.

Abstract: The Escherichia coli AcrAB-TolC efflux pump is the archetype of the resistance nodulation cell division (RND) exporters from Gram-negative bacteria. Overexpression of RND-type efflux pumps is a major factor in multidrug resistance (MDR), which makes these pumps important antibacterial drug discovery targets. We have recently developed novel pyranopyridine-based inhibitors of AcrB, which are orders of magnitude more powerful than the previously known inhibitors. However, further development of such inhibitors has been hindered by the lack of structural information for rational drug design. Although only the soluble, periplasmic part of AcrB binds and exports the ligands, the presence of the membrane-embedded domain in AcrB and its polyspecific binding behavior have made cocrystallization with drugs challenging. To overcome this obstacle, we have engineered and produced a soluble version of AcrB [AcrB periplasmic domain (AcrBper)], which is highly congruent in structure with the periplasmic part of the full-length protein, and is capable of binding substrates and potent inhibitors. Here, we describe the molecular basis for pyranopyridine-based inhibition of AcrB using a combination of cellular, X-ray crystallographic, and molecular dynamics (MD) simulations studies. The pyranopyridines bind within a phenylalanine-rich cage that branches from the deep binding pocket of AcrB, where they form extensive hydrophobic interactions. Moreover, the increasing potency of improved inhibitors correlates with the formation of a delicate protein- and water-mediated hydrogen bond network. These detailed insights provide a molecular platform for the development of novel combinational therapies using efflux pump inhibitors for combating multidrug resistant Gram-negative pathogens.

A Fast and Accurate PMU Algorithm for P+M Class Measurement of Synchrophasor and Frequency

Abstract: The IEEE Standard C37.118.1 defines two performance classes, P and M, for phasor measurement units (PMUs), respectively for protection and monitoring oriented applications. The goal of this paper is to define an algorithm that allows the requirements of both classes to be met simultaneously, thus avoiding an a priori selection of either the fast response time of class P or the accuracy of the class M. The designed PMU consists of two digital channels that process in parallel the acquired samples with different algorithms: the first one allows accurate measurements of steady state signals, while the second one is better suited to follow the fast signal changes. Then, a detector identifies the possible presence of dynamic situations and selects the most appropriate output for the actual operating condition. The validation of the solution, performed by means of simulations in all the static and dynamic conditions defined in the standard, confirms that the method is able to comply with all the limits indicated for both classes for synchrophasor and frequency measurement. As for the rate of change of frequency, again the compliance to P-class is fully verified, while for the M-class the only exception is represented by the tests with out-of-band disturbances.