Showing papers by "University of Colorado Denver published in 1972"
TL;DR: Dibutyryl cyclic AMP has been shown to restore controlled growth to transformed cells3, change the cell's surface architecture3, and induce axon formation with an accompanied increase in acetylcholinesterase activity in neuroblastoma cells growing in culture, suggesting that the cyclicAMP moiety may have some basic regulatory action on cell growth and cell specialization.
Abstract: CYCLIC 3′5′-adenosine monophosphate (cyclic AMP) regulates many physiological phenomena1,2. Cellular morphology changes when the dibutyryl derivative of cyclic AMP is added in vitro to the nutrient media of cultured mammalian cells3–6. Dibutyryl cyclic AMP has also been shown to restore controlled growth to transformed cells3, change the cell's surface architecture3,7 and induce axon formation8 with an accompanied increase in acetylcholinesterase activity9 in neuroblastoma cells growing in culture. These effects suggest that the cyclic AMP moiety may have some basic regulatory action on cell growth and cell specialization.
367 citations
252 citations
TL;DR: Growth stimulated by protease treatment of untransformed cells is correlated with a decrease in cell cAMP levels and can be prevented by dibutyryl cAMP and changed growth characteristics of transformed cells are discussed.
Abstract: Growth stimulated by protease treatment of untransformed cells is correlated with a decrease in cell cAMP levels and can be prevented by dibutyryl cAMP. The changed growth characteristics of transformed cells are discussed with regard to cAMP levels.
243 citations
TL;DR: It is proposed that a program of directed masturbation can be effective in treating primary orgasmic dysfunction and techniques for dealing with clients' negative emotions towards masturbation are discussed.
Abstract: This chapter reports on the use of masturbation in the treatment of women who have never experienced orgasm (primary orgasmic dysfunction). A nine-step program was developed by the authors that includes directed masturbation in conjunction with sensate pleasuring and other therapy procedures.
218 citations
TL;DR: It is postulate that increased tensile forces on the collagen bundles caused by muscle contraction tighten the braided collagen bundles thereby squeezing and distorting the axon terminals.
Abstract: The Golgi tendon organ (GTO) has a capsule composed of cells confluent with the perineural epithelial sheath surrounding the Ib afferent nerve. Fluids of the capsule lumen are isolated from extra-capsular fluids by the capsule wall; its tight-fitting collars form seals through which collagen bundles enter and leave the proximal and distal ends of the fusiform capsule. The capsule lumen between the sealed capsule openings, is divided into longitudinal compartments by delicate processes of septal cells. Collagen bundles spiral down through the longitudinal axis of the GTO. As they descend through the lumen, the bundles are distinctly separated from each other by fluid-filled spaces. These collagen bundles divide, twist, and regroup over short longtiudinal distances and unmyelinated axons interwine among them. Compartments which are densely filled with collagen are not innervated. Axon profiles appear in four forms: (I) myelinated branches of Ib axon, (II) unmyelinated axons surrounded by Schwann cell processes and basal lamina, (III) axons covered only by basal lamina, and (IV) axons with bare surfaces. We postulate that increased tensile forces on the collagen bundles caused by muscle contraction tighten the braided collagen bundles thereby squeezing and distorting the axon terminals. The proposed mechanical events are consistent with known discharge characteristics of Ib axons.
129 citations
TL;DR: Isoleucine deficiency, which blocks cells in the G1 period, led to a depressed level of cAMP, possibly due to the inhibited protein synthesis which accompanies isoleucune deficiency.
96 citations
TL;DR: Treatment with phenobarbital of rats which are genetically selected results in a greater than 10-fold increase in nicotinamide-adenine dinucleotide (NAD)-dependent aldehyde dehydrogenase present in the supernatant but not the mitochondrial fraction of liver.
95 citations
TL;DR: Although a maximal differentiation of cells was seen 24 h after papaverine treatment, a maximal inhibition of cell division was observed 2 days after treatment, which supports the hypothesis that the inhibition ofcell division may be secondary to the induction of differentiation.
94 citations
86 citations
TL;DR: Hydrocortisone treatment results in the appearance in bone marrow of additional PHA-responsive cells which are sensitive to anti-θ, and which are presumably derived from other peripheral lymphoid tissues.
75 citations
TL;DR: It was concluded that resistance of the membrane ATPase to DCCD was due to a defective carbodiimide-sensitizing factor, the membrane component that mediates D CCD inhibition.
TL;DR: The five essential steps involved in large-scale chromosome analysis, namely automatic scanning, manual pictorial editing, classical computer analysis of chromosomes, banding computerAnalysis of chromosomes and statistical analysis of chromosomal data are all discussed.
TL;DR: Mice pretreated with soluble NIP-MγG and challenged with NIP coupled to a heterologous carrier give a normal antibody response to the carrier but have barely detectable levels of antibody to NIP, which implies that thymus- derived cells as well as bone marrow-derived cells are involved in hapten-specific tolerance.
Abstract: A hapten, 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP) when coupled to isologous mouse gamma globulin (MγG) elicits a hapten-specific immune response in mice if administered in Freund's complete adjuvant. This response is measurable by the capacity of the sera to bind N125IP, by detection of NIP-specific plaque-forming cells (B cells), and by in vitro secondary type antigen-driven DNA synthesis (T cells and probably B cells). The in vitro response requires both the hapten and carrier since neither by itself is capable of stimulating the spleen cells. This same antigen gives rise to hapten-specific tolerance when given in the soluble form. Mice pretreated with soluble NIP-MγG and challenged with NIP coupled to a heterologous carrier give a normal antibody response to the carrier but have barely detectable levels of antibody to NIP. Spleen cells from mice made tolerant to NIP-MγG do not respond in vitro with increased DNA synthesis. This implies that thymus-derived cells as well as bone marrow-derived cells are involved in hapten-specific tolerance.
Journal Article•
TL;DR: It is concluded that the virus-induced CPE and stimulation of phosphatidylcholine synthesis are not consequences of the inhibition of cellular RNA or protein synthesis, and suggests that the CPE may be induced by a viral protein precursor.
Abstract: L cells were infected with Mengo virus in the presence of varying concentrations of protein synthesis inhibitors (azetidine-2-carboxylic acid, p-fluorophenylalanine, puromycin), and examined with respect to the effects of the inhibitors on several features of virus-induced cell injury. The virus-specific events in the cells could be dissociated into three groups, based on their sensitivity to the inhibitors: (i) viral ribonucleic acid (RNA) synthesis, bulk viral protein synthesis, and infectious particle production, all of which were prevented by low inhibitor concentrations; (ii) the cytopathic effect (CPE) and stimulation of phosphatidylcholine synthesis, which were sensitive to intermediate concentrations of the inhibitors; and (iii) the virus-induced inhibitions of host RNA and protein synthesis, which were resistart to the inhibitors of protein synthesis except at very high concentrations. It is concluded from this that the virus-induced CPE and stimulation of phosphatidylcholine synthesis are not consequences of the inhibition of cellular RNA or protein synthesis. Analysis of the virus-specific protein and RNA synthesized at several concentrations of azetidine and puromycin suggests that the CPE may be induced by a viral protein precursor. Virus-induced inhibition of host RNA and protein synthesis occurred at azetidine concentrations which blocked the synthesis of over 99.7% of the total viral RNA and over 99% of the viral double-stranded RNA (dsRNA). Calculations show that this would correspond to less than 150 dsRNA molecules per infected cell, resulting in a dsRNA-polysome ratio of less than 1:1,000; this indicates that host protein synthesis cannot be inhibited by an irreversible binding of dsRNA to polysomes.
TL;DR: If cholecystoduodenostomy is used in future cases, prompt re-exploration and conversion to choledochoenterostomy should be considered if the diagnosis of duct obstruction, cholangitis, and persistent bacteremia are made.
TL;DR: The capacity of heart, lung, brain, heart and testes tissues to reduce m -nitrobenzaldehyde was 2- to 10-fold greater than the capacity to reduce propionaldehyde with either NADH or NADPH, however, aldehyde reductase activity in liver tissue was greater with propional dehyde than with m-nitro Benzaldehyde as substrate.
TL;DR: It is suggested that in selecting patients for inclusion in a home dialysis training program, in many cases the program will have to make a decision about which is more valued; ‘quantity’ of life (sheer length of physical survival), or ‘quality of life’ (emotional adjustment while alive).
01 Jan 1972
TL;DR: This procedure of priming animals with portions of electroimmunodiffusion-produced arcs of antigen-antibody precipitate and later boosting them with crude antigen mixture thus provides a simple, effective means for preparing antisera with chosen restricted specificities without preliminary, frequently difficult conventional biochemical purifications of antigens.
Abstract: Miniaturized two-dimensional single electroimmunodiffusion (2-D EID) was used to produce directly and simply minute quantities of purified human serum antigens complexed with their precipitins. These complexes injected in incomplete Freund adjuvant readily primed rabbits for vigorous selective production of precipitins to the complexed antigens when the animals later were boosted with whole human serum. This procedure of priming animals with portions of electroimmunodiffusion-produced arcs of antigen-antibody precipitate and later boosting them with crude antigen mixture thus provides a simple, effective means for preparing antisera with chosen restricted specificities without preliminary, frequently difficult conventional biochemical purifications of antigens. The technique was unexpectedly sensitive, readily detecting native associations between certain serum antigens (e.g., between albumin and both β-lipoprotein and α2-macroglobulin). Thus rabbits primed with nearly 100,000-fold differences in quantiti...
TL;DR: The effect of protein denaturant urea upon the circular dichroism spectrum of lysozyme in aqueous solution has been determined in this article, showing that the negative plateau in the near-ultraviolet spectrum of native Lysozyme arises primarily from the intramolecular disulfide bonds of the protein.
TL;DR: It is suggested that in the failure of human liver allografts cell-mediated immunity and non-immunological factors may be more important than humoral antibody.
TL;DR: A pyramidal plan of therapy is set forth which may help in realistic planning of rheumatoid arthritis treatment, using steroids, orthopedic devices, gold salts, hospitalization for intensive care, and surgery.
Abstract: Two questions are often asked about treatment of rheumatoid arthritis: What do you use? When do you use specific measures? Because the problems differ so widely from case to case, optimal management is problem-oriented. A pyramidal plan of therapy is set forth which may help in realistic planning. At the base of the pyramid are heat, exercise, rest, salicylates and education. Depending on the severity of disease and the response to the basic program, one may or may not move up the pyramid, using steroids, orthopedic devices, gold salts, hospitalization for intensive care, and surgery.
TL;DR: Cow adrenal medulla chromaffin granules were obtained relatively free of mitochondrial, lysosomal or mircosomal contamination by differential and sucrose density gradient centrifugation and injection of the membrane preparation into rabbits resulted in the production of an antibody which was used for micro-immunodiffusion and for complement fixation.
TL;DR: The data support the conclusion that the native state of the flavin is a dinucleotide, similar to that isolated previously from succinate dehydrogenase of beef and pig heart mitochondria.
TL;DR: The metabolism in mice of 4-methylpyrazole (4-MP), a potent inhibitor of alcohol dehydrogenase, has been investigated using gas chromatography and gas Chromatography-mass spectrometry techniques and radioactive 4-MP was synthesized to aid in the isolation of the metabolites.
TL;DR: Investigating the cellular effect of cortisol on tuberculin reactions in guinea pigs revealed that cortisol treatment resulted in a greater in a reduction in macrophages than small lymphocytes, which shows that either the sensitized Iymphocyte is unable to produce and release MIF or the macrophage itself cannot respond to MIF when treated with cortisol.
TL;DR: The results indicate that these gestational hormones do not produce additive effects on glucosestimulated insulin secretion or on total islet insulin content.
Abstract: Insulin secretion after glucose stimulation and residual insulin content was studied in islets of Langerhans isolated from adult female rats which had been treated for 1–2 weeks with progesterone (P),estriol (E3), 17β-estradiol (E2), human chorionic somatomammotropin (HCS), E3 + P, E2 + P, HCS + P, or E3 + HCS + P. An increase in insulin secretion was observed after P treatment which was not further augmented by E3 + P and was reversed by E2 + P treatment. E2 treatment alone led to a smaller than normal insulin secretion response to glucose stimulation. Blunting of P-enhanced glucose-stimulated insulin secretion was also observed after E3 + HCS + P, but not after HCS + P. Residual islet insulin content increased after P and E3 + P, but not after E2 + P administration. These results indicate that these gestational hormones do not produce additive effects on glucosestimulated insulin secretion or on total islet insulin content. (Endocrinology 91: 977, 1972)