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Showing papers by "University of Córdoba (Spain) published in 1983"


Journal ArticleDOI
TL;DR: In this paper, data for gross chemical composition, energy, nitrogen distribution, major minerals, water activity, and pH in ten representative varieties of Spanish cheeses were reported for gross and gross chemical compositions.

38 citations


Journal ArticleDOI
TL;DR: Two MAO isoenzymes apparently exist in terrestrial tetrapods, but only one form (type A-like) can be detected in teleosts and in aquatic amphibia.
Abstract: 1. 1. Monoamine oxidase (MAO) activity is high in brain, where it regulates neurotransmitter levels, and in the ‘detoxifying’ organs. 2. 2. Two MAO isoenzymes (A and B) apparently exist in terrestrial tetrapods, but only one form (type A-like) can be detected in teleosts and in aquatic amphibia. 3. 3. MAO activity is regulated by both endogenous (hormones, substrates) and exogenous (daylength, temperature) factors.

34 citations


Journal ArticleDOI
TL;DR: Esterase lipase (C8), leucine arylamidase, acid phosphatase, and phosphoamidase were detected in all of the isolates while trypsin and α-galactosidases were not found in any of the isolate using this system.
Abstract: The enzymatic profiles of several yeastlike organisms were studied using 19 substrates included in the API ZYM system. The isolates evaluated were: 186 Candida albicans, 19 C. stellatoidea, 4 C. tropicalis, 2 C. parapsilosis, 2 C. pseudotropicalis, 1 C. guilliermondii, 3 C. krusei, 11 Torulopsis (Candida) glabrata, 1 Cryptococcus neoformans, 2 Saccharomyces carlsbergensis, 1 Rhodotorula rubra, and 1 R. mucilaginosa.

30 citations


Journal ArticleDOI
TL;DR: In vitro tests for pollen germination and pollen tube growth of olive cultivars were carried out with the addition of pistil extracts, finding that low pistil extract concentrations (.0.3–0.6 mg ml-1 f.w.) enha...
Abstract: In vitro tests for pollen germination and pollen tube growth of olive cultivars were carried out with the addition of pistil extracts. Low pistil extract concentrations (.0.3–0.6 mg ml-1 f.w.) enha...

30 citations


Journal ArticleDOI
01 Feb 1983-Talanta
TL;DR: A kinetic method is described for the determination of trace amounts of manganese(II), based on its catalytic effect on the oxidation of 2-hydroxybenzaldehyde thiosemicarbazone by hydrogen peroxide, with a precision of +/-1%.

29 citations


Journal ArticleDOI
TL;DR: In this paper, two expressions have been developed to determine the half-wave potentials of such waves assuming diffusion-controlled processes, both for reversible and irreversible transferences in a homogeneous phase.

25 citations


Journal ArticleDOI
TL;DR: 2,6-dichlorobenzonitrile inhibits cytokinesisin vivo in meristem cells of onion root tips being this inhibition non-permanent in nature, and a model for cytokinesis in which at least two cytophysiological pathways occur is proposed.
Abstract: 2,6-dichlorobenzonitrile inhibits cytokinesisin vivo in meristem cells of onion root tips being this inhibition non-permanent in nature. Results from dual inhibition treatments of dichlobenil and caffeine suggest that dichlobenil non-permanently inhibits a metabolic cytokinesis pathway which differs from that inhibited permanently by caffeine. We propose a model for cytokinesis in which at least two cytophysiological pathways occur.

25 citations


Journal ArticleDOI
TL;DR: Prototheca wickerhamii was isolated from the stool of a two month-old infant with diarrhea resistant to treatment, the first description from Spain of algae of the genus Prototheca isolated from human clinical specimens.
Abstract: Prototheca wickerhamii was isolated from the stool of a two month-old infant with diarrhea resistant to treatment This is the first description from Spain of algae of the genus Prototheca isolated from human clinical specimens The possible pathogenicity of the Prototheca in this case is discussed

20 citations


Journal ArticleDOI
TL;DR: A synthetic model is suggested that explains both the positioning and timing of cortical morphogenesis in the cell cycle and the attribution to the AZM of a repressive capacity on the stomatogenic area according to the system of gradients of morphogenetic activity proposed by Jerka-Dziadosz to explain location of primordia in urostylids.
Abstract: Morphogenetic events during division, physiological reorganization, and postraumatical regeneration, the last being induced both chemically and microsurgically, were studied by light microscopy on protargol-impregnated specimens of the hypotrichous ciliate, Laurentiella acuminata. Parakinetal stomatogenesis, from transverse cirrus-1 during division and reorganization, changes during regeneration to a parakinetal one which characterizes more primitive members of Hypotrichida in the S. O. Stichotrichina, but solely when the AZM is damaged. These morphogenetic events a) confirm the previous inclusion of L. acuminata among the Oxytrichidae on the basis of its morphological characters and indicate that it is a primitive species of this family related with the Stichotrichina through genera Pleurotricha and Paraurostyla; b) suggest a synthetic model that explains both the positioning and timing of cortical morphogenesis in the cell cycle. The key point of this model is the attribution to the AZM of a repressive capacity on the stomatogenic area, the last one being positioned according to the system of gradients of morphogenetic activity proposed by Jerka-Dziadosz to explain location of primordia in urostylids. This repression is manifested not as a gradient, as indicated by De Terra, but as a long-term repression limited to a certain distance. Simultaneous repression and stimulation occurring in a growing cortex with the AZM remaining constant in size could explain the critical ratio, buccal cortex/somatic cortex, at which stomatogenesis is triggered as indicated by De Terra.

19 citations



Journal ArticleDOI
TL;DR: In this paper, a comparative study of 2-benzoylpyridine hydrazone and azine has been carried out by using classic and differential pulse polarography techniques, and mechanisms for the reduction of the compounds are proposed.

Journal ArticleDOI
TL;DR: The apparent Michaelis constant of MAO activity in the different groups and tissues was generally similar, suggesting a similarity of the MAO molecule.

Journal ArticleDOI
TL;DR: In this article, a mechanism for the electroreduction process based on the data obtained by means of the differential pulse polarography technique is proposed, and resolution of these isomers has been obtained.

Journal ArticleDOI
TL;DR: In this article, the use of different equations proposed to study the kinetics of the glass-to-crystal transition from DTA curves has been extended to include the evaluation of the kinetic parameters of polymorphic transitionS.
Abstract: The use of the different equations proposed to study the kinetics of the glass-to-crystal transition from DTA curves has been extended to include the evaluation of the kinetic parameters of polymorphic transitionS. The transformation kinetics of CaCO3 (aragonite to calcite) and PbO (litharge to massicot) are the examples studied. The kinetic results of both transformations, obtained by the analysis of DTA curves, differ widely from those revealed by the analysis of the isothermal kinetic data. This discrepancy is probably due to erroneous simplifications used in deriving the equations applied in non-isothermal conditions.

Journal ArticleDOI
TL;DR: In this article, the authors used linear regression equations to predict water activity of San Simon cheese with an absolute error not greater than±.01 often found in measurements of water activity by different methods.

Journal ArticleDOI
TL;DR: In this paper, an electrode process of catalytic character obtained with the cobalt ion-2-benzoylpyridine oxime system was studied by differential pulse polarization.

Journal ArticleDOI
TL;DR: This paper is concerned with two fundamental aspects of this paper: the conceptual and technical differences among objectives, goals, and constraints are not clearly established, and the possible effects of setting pessimistic levels of aspiration in a goal-programming problem are not analyzed.
Abstract: In a recent Journal article Barnett, Blake, and McCarl (BBMcC) provide an interesting and welcome application of linear goal programming. Until recently agricultural economists have been slow in the use of this and similar techniques in farm planning. However, we are concerned with two fundamental aspects of this paper. First, the conceptual and technical differences among objectives, goals, and constraints are not clearly established. This creates difficulties of identifying, unambiguously, situations to which techniques of multiple-goal and multiple-objectives should be applied. Second, the possible effects of setting pessimistic levels of aspiration in a goal-programming problem, allowing goals to be satisfied readily, has not been analyzed.

Journal ArticleDOI
TL;DR: The in vitro activity of ribostamycin against algae of the genus Prototheca was evaluated and it is likely that this antibiotic could be effective in the treatment of protothecosis in man.
Abstract: The in vitro activity of ribostamycin against algae of the genus Prototheca was evaluated by minimum inhibitory concentration (MIC) studies on solid media. Concentrations of 4 mcg/ml were required to inhibit 100% of the P. zopfii strains; 16 mcg/ml inhibited 100% of the P. stagnora strains and 95% of the P. wickerhamii strains. These values are inferior to plasma concentrations obtained after injection of ribostamycin. It is likely that this antibiotic could be effective in the treatment of protothecosis in man.

Journal ArticleDOI
TL;DR: Four-week-old sunflower plants (Helianthus annuus L. cv. Halcon), grown in different nutrient solutions, were used to study the effects of gibberellic acid (GA3) on K+ (Rb+) uptake by roots or transport to the shoot, and it is suggested that these GA3 effects can be explained if it is assumed that GA3 acts on the transport of ions to the xylem vessels.
Abstract: Four-week-old sunflower plants (Helianthus annuus L. cv. Halcon), grown in different nutrient solutions, were used to study the effects of gibberellic acid (GA3) on K+ (Rb+) uptake by roots or transport to the shoot. Gibberellic acid application to the nutrient solution did not affect the exudation process of excised roots. When GA3 was sprayed on leaves 2 to 6 days before excising the roots, the rate of exudation and the K+ flux increased. When the exudation study was done keeping the roots in a nutrient solution in which Rb+ replaced K+, the GA3 effects were evident also on Rb+ uptake and transport. In intact plants, GA3 increased the Rb+ transported to the shoot but did not affect Rb+ accumulation in the root. It is suggested that these GA3 effects can be explained if it is assumed that GA3 acts on the transport of ions to the xylem vessels.


Journal ArticleDOI
TL;DR: The kinetic and enzymatic parameters are similar to those previously reported for the diaphorase activity of the whole complex, which indicates that the NAD(P)H-cytochrome c reductase of 104 mutant is a true subunit of the Chlamydomonas reinhardii NAD(H-nitrate reduct enzyme native enzyme.

Journal ArticleDOI
TL;DR: In this paper, the electrochemical reduction of monoethyl maleate (MEM) on a mercury electrode has been carried out and two waves were found by dc polarography and two peaks by dpp.

Journal ArticleDOI
TL;DR: It is proposed that nitrate reductase from mutant 305 has a protein deletion which affects the pyridine nucleotide binding region of the diaphorase protein but without any effect on the terminal nitrate reducectase activity.
Abstract: Nitrate reductase from mutant 305 of Chlamydomonas reinhardii has been purified about 90-fold and biochemically characterized. The enzyme can use reduced flavins and viologens as electron donors to reduce nitrate but, unlike the nitrate reductase complex from its parental wild strain, lacks NAD(P)H-nitrate reductase and NAD(P)H-cytochrome c reductase activities, does not bind to Blue-Agarose or Blue-Sepharose and exhibits a significantly lower molecular weight (177.000 vs. 241.000), whereas its kinetic characteristics and its sensitivity against several inhibitors and treatments are very similar to those of the terminal nitrate reductase activity of the wild strain complex. Spectral studies and antagonistic experiments with tungstate show the presence of cytochrome b557 and molybdenum. These facts lead us to propose that nitrate reductase from mutant 305 has a protein deletion which affects the pyridine nucleotide binding region of the diaphorase protein but without any effect on the terminal nitrate reductase activity.

Journal ArticleDOI
TL;DR: Pyridoxal salicyloylhydrazone reacts with titanium(IV) in acidic medium to produce an orange 1∶1 complex (λmax=390 nm, shoulder at 440-450 nm) The fundamental analytical properties of the complex in aqueous solution and the optimal conditions for a selective spectrophotometric determination of titanium are described as discussed by the authors.
Abstract: Pyridoxal salicyloylhydrazone reacts with titanium(IV) in acidic medium (pH=09–25) to produce an orange 1∶1 complex (λmax=390 nm, shoulder at 440–450 nm) The fundamental analytical properties of the complex in aqueous solution and the optimal conditions for a selective spectrophotometric determination of titanium are described Interferences for numerous foreign ions have been investigated The method has been successfully applied to the determination of titanium in various standard samples

Journal ArticleDOI
TL;DR: In this article, an AAc 2 pathway has been established for the esterification of benzoic acid with ethanol catalyzed by AlPO4 and related systems, where the rate-controlling step is the reaction of an adsorbed BAs with an ethanol molecule from the gas phase.
Abstract: The esterification of benzoic acid with ethanol catalyzed by AlPO4 and related systems has been studied. An AAc 2 pathway has been established in all cases. The rate-controlling step is the reaction of an adsorbed benzoic acid molecule with an ethanol molecule from the gas phase.

Journal ArticleDOI
TL;DR: In this article, zinc and cadmium are extracted from aqueous solution with 1,2-naphthoquinone thiosemicarbazone for simultaneous determination by atomic absorption spectrophotometry.
Abstract: Zinc and cadmium are extracted from aqueous solution with 1,2-naphthoquinone thiosemicarbazone for simultaneous determination by atomic-absorption spectrophotometry This compound reacts with zinc and cadmium in weakly acid medium to produce chelates which are extractable into methyl isobutyl ketone The atomicabsorption is measured at 2139 and 2288nm for zinc and cadmium, respectively The sensitivity is 03 ng per ml of original aqueous solution and several foreign ions are tolerated in 100-fold ratio to Zn or Cd

Book ChapterDOI
01 Jan 1983
TL;DR: There is genetic variability between species or genotypes in their adaptation to soil with high calcium or magnesium content, but little is known about the physiological basis of these adaptations.
Abstract: There is genetic variability between species or genotypes in their adaptation to soil with high calcium or magnesium content3,6 but little is known about the physiological basis of these adaptations. Clark1 found differential Ca and Mg efficiencies between corn inbreds, and Kawaski and Moritsugu5 have shown differences in the absorption of Ca by excised root of different corn and sorghum cultivars.

Journal ArticleDOI
TL;DR: By combining chromatographic procedures and preparative isoelectric focusing, three major fractions of NAD(P)H-cytochrome c reductase were isolated with pi at 7.1, 5.5 and 5.05, respectively, and the last one was identified as the diaphorase subunit of the NAD(H-nitrate reduCTase by its ability to reconstitute in vitro the nitrate reductases native complex.
Abstract: Chlamydomonas reinhardii 104 mutant strain cells contain many NAD(P)H-cytochrome c reductase activities unrelated to nitrate reduction in addition to an NAD(P)H-cytochrome c reductase related to NAD(P)H-nitrate reductase, which have molecular weights from 37000 to 124000. By combining chromatographic procedures and preparative isoelectric focusing, three major fractions of NAD(P)H-cytochrome c reductase were isolated with pi at 7.1, 5.5 and 5.05, respectively. Of these fractions, only the last one was identified as the diaphorase subunit of the NAD(P)H-nitrate reductase by its ability to reconstitute in vitro the nitrate reductase native complex upon complementation with active terminal nitrate reductase of 305 mutant of C. reinhardii.

Journal ArticleDOI
TL;DR: In this paper, the authors present a computer calculation method which permits the determination of the elevation angle with respect to the equator for each position adopted by a quasi-static collector, and also the calculation of the minimum collector area to be installed to achieve the maximum agreement between the curves of energy needed and collected with no seasonal accumulation.

DOI
31 Dec 1983
TL;DR: Se describe el macho de Messor hispanicus a partir de ejemplares localizados cerca oficiales de la capital cordobesa y realiza una comparacion con otros machos de especies of Messor presentes en the zona.
Abstract: Se describe el macho de Messor hispanicus a partir de ejemplares localizados cerca de la capital cordobesa. Se realiza ademas una comparacion con otros machos de especies de Messor presentes en la zona.