University of Córdoba (Spain)

About: University of Córdoba (Spain) is a education organization based out in Cordova, Spain. It is known for research contribution in the topics: Population & Catalysis. The organization has 12006 authors who have published 22998 publications receiving 537842 citations. The organization is also known as: University of Córdoba (Spain) & Universidad de Córdoba.

Lipotoxicity and Diabetic Nephropathy: Novel Mechanistic Insights and Therapeutic Opportunities

Abstract: Lipotoxicity is characterized by the ectopic accumulation of lipids in organs different from adipose tissue. Lipotoxicity is mainly associated with dysfunctional signaling and insulin resistance response in non-adipose tissue such as myocardium, pancreas, skeletal muscle, liver, and kidney. Serum lipid abnormalities and renal ectopic lipid accumulation have been associated with the development of kidney diseases, in particular diabetic nephropathy. Chronic hyperinsulinemia, often seen in type 2 diabetes, plays a crucial role in blood and liver lipid metabolism abnormalities, thus resulting in increased non-esterified fatty acids (NEFA). Excessive lipid accumulation alters cellular homeostasis and activates lipogenic and glycogenic cell-signaling pathways. Recent evidences indicate that both quantity and quality of lipids are involved in renal damage associated to lipotoxicity by activating inflammation, oxidative stress, mitochondrial dysfunction, and cell-death. The pathological effects of lipotoxicity have been observed in renal cells, thus promoting podocyte injury, tubular damage, mesangial proliferation, endothelial activation, and formation of macrophage-derived foam cells. Therefore, this review examines the recent preclinical and clinical research about the potentially harmful effects of lipids in the kidney, metabolic markers associated with these mechanisms, major signaling pathways affected, the causes of excessive lipid accumulation, and the types of lipids involved, as well as offers a comprehensive update of therapeutic strategies targeting lipotoxicity.

Selecting patterns and features for between- and within- crop-row weed mapping using UAV-imagery

Abstract: The problem of remote weed mapping via machine learning is considered.Unmanned aerial vehicles are used to capture maize and sunflower field images.The proposed method considers pattern and feature selection techniques.The final model requires few user information to generalise to new areas.There are features of great influence for the classification of both crops. This paper approaches the problem of weed mapping for precision agriculture, using imagery provided by Unmanned Aerial Vehicles (UAVs) from sunflower and maize crops. Precision agriculture referred to weed control is mainly based on the design of early post-emergence site-specific control treatments according to weed coverage, where one of the most important challenges is the spectral similarity of crop and weed pixels in early growth stages. Our work tackles this problem in the context of object-based image analysis (OBIA) by means of supervised machine learning methods combined with pattern and feature selection techniques, devising a strategy for alleviating the user intervention in the system while not compromising the accuracy. This work firstly proposes a method for choosing a set of training patterns via clustering techniques so as to consider a representative set of the whole field data spectrum for the classification method. Furthermore, a feature selection method is used to obtain the best discriminating features from a set of several statistics and measures of different nature. Results from this research show that the proposed method for pattern selection is suitable and leads to the construction of robust sets of data. The exploitation of different statistical, spatial and texture metrics represents a new avenue with huge potential for between and within crop-row weed mapping via UAV-imagery and shows good synergy when complemented with OBIA. Finally, there are some measures (specially those linked to vegetation indexes) that are of great influence for weed mapping in both sunflower and maize crops.

Laboratory evaluation of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against puparia and adults of Ceratitis capitata (Diptera: Tephritidae).

Abstract: Laboratory experiments were done to measure the pathogenicity of 10 autochthonous isolates of Beauveria bassiana (Balsamo) Vuill. and of five Metarhizium anisopliae (Metsch.) Sorok. toward puparia and adults of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Although all isolates applied via inoculation of the fungal suspensions on the ventral surface of the abdomen were pathogenic to adults, with mortality rates ranging from 30 to 100% and average survival times (ASTs) from 6.5 to 8.6 d, when C. capitata puparia were immersed in the conidial suspensions, only B. bassiana Bb-1333 and EABb 01/103-Su and M. anisopliae EAMa 01/58-Su isolates caused >50% mortality of puparia. In a second series of bioassays conducted on five selected isolates, adults were sprayed with four 10-fold concentrations ranging from 1.0 x 10(5) to 1.0 x 10(8) colony-forming units (cfu)/ml. The median lethal concentrations (LC50) of the four most virulent isolates ranged from 4.9 x 10(5) to 2.0 x 10(6) cfu/ml with estimated time to kill 50% of the insects ranging from 4.6 to 5.3 d. The effect of a sublethal dose (ca. LD50) of either B. bassiana EABb 01/103-Su or M. anisopliae EAMa 01/58-Su isolate was studied by reciprocal crossing. Treatment with B. bassiana reduced fecundity and fertility at 6, 8, and 10 d after treatment, with fecundity and fertility reductions ranging from 20.0 to 71.2% and from 33.6 to 60.0%, respectively. These reductions occurred in pairing combinations of treated females with either treated or nontreated males. M. anisopliae was more effective in reducing fecundity and fertility at 6 d after treatment, with the reduction varying from 58.4 to 72.1% and from 28.6 to 45.9%, respectively. In addition, the first oviposition was significantly delayed for 1 d in females treated by either fungal species. The above-mentioned five selected isolates were assayed against C. capitata puparia treated as late third instars in sterilized soil at 25'C under three moisture conditions (-0.1, -0.01, and -0.0055 MPa). At -0.01 MPa, all isolates were low pathogenic to C. capitata puparia, whereas significant differences in the puparia mortality occurred between isolates at -0.1 and -0.0055 MPa. The highest pupal mortalities ranged from 52.5 to 70.0%, as a function of soil moisture and were caused by EAMa 01/58-Su and Bb-1333 isolates.

Magnesium inhibits Wnt/β-catenin activity and reverses the osteogenic transformation of vascular smooth muscle cells.

Abstract: Magnesium reduces vascular smooth muscle cell (VSMC) calcification in vitro but the mechanism has not been revealed so far. This work used only slightly increased magnesium levels and aimed at determining: a) whether inhibition of magnesium transport into the cell influences VSMC calcification, b) whether Wnt/β-catenin signaling, a key mediator of osteogenic differentiation, is modified by magnesium and c) whether magnesium can influence already established vascular calcification. Human VSMC incubated with high phosphate (3.3 mM) and moderately elevated magnesium (1.4 mM) significantly reduced VSMC calcification and expression of the osteogenic transcription factors Cbfa-1 and osterix, and up-regulated expression of the natural calcification inhibitors matrix Gla protein (MGP) and osteoprotegerin (OPG). The protective effects of magnesium on calcification and expression of osteogenic markers were no longer observed in VSMC cultured with an inhibitor of cellular magnesium transport (2-aminoethoxy-diphenylborate [2-APB]). High phosphate induced activation of Wnt/β-catenin pathway as demonstrated by the translocation of β-catenin into the nucleus, increased expression of the frizzled-3 gene, and downregulation of Dkk-1 gene, a specific antagonist of the Wnt/β-catenin signaling pathway. The addition of magnesium however inhibited phosphate-induced activation of Wnt/β-catenin signaling pathway. Furthermore, TRPM7 silencing using siRNA resulted in activation of Wnt/β-catenin signaling pathway. Additional experiments were performed to test the ability of magnesium to halt the progression of already established VSMC calcification in vitro. The delayed addition of magnesium decreased calcium content, down-regulated Cbfa-1 and osterix and up-regulated MGP and OPG, when compared with a control group. This effect was not observed when 2-APB was added. In conclusion, magnesium transport through the cell membrane is important to inhibit VSMC calcification in vitro. Inhibition of Wnt/β-catenin by magnesium is one potential intracellular mechanism by which this anti-calcifying effect is achieved.

Utilisation of unbound recycled aggregates from selected CDW in unpaved rural roads

Abstract: This article evaluates the behaviour and environmental impact of two recycled aggregates from selected construction and demolition waste (CDW) in field conditions. For this purpose, one experimental unpaved rural road with two sections was built in 2007. Sections were formed with an excellent subgrade (A-1-b) and two structural layers: the first section consisted of a base course and a surface built using a mixed recycled aggregate and a recycled concrete aggregate, respectively, and the second section consisted of crushed limestone aggregate as a reference. The materials were previously characterised in the laboratory. Control compaction ensured that the materials were correctly set in place, and the bearing capacity of each of the fill layers was measured. The structural performance of the pavement was determined using a Falling Weight Deflectometer, and changes over time in the International Roughness Index and the bearing capacity were studied. The results show that recycled aggregates from selected CDW can be used as an alternative to natural aggregates in unpaved rural road construction without risk of environmental impact. According to technical specifications, the soluble salt content can rise to 1.3% without reducing the quality of this type of road. This study is important for increasing recycling rates and creating a market for mixed recycled aggregates in Mediterranean countries such as Spain, which has one of the lowest recycling rates in Europe.