University of Costa Rica

About: University of Costa Rica is a education organization based out in San José, Costa Rica. It is known for research contribution in the topics: Population & Venom. The organization has 9817 authors who have published 16781 publications receiving 238208 citations. The organization is also known as: UCR & Universidad de Costa Rica.

Emergence of Clostridium difficile NAP1 in Latin America

Abstract: The NAP1 and NAP2 strains of Clostridium difficile have been linked to nosocomial outbreaks of antibiotic-associated diarrhea (AAD) and pseudomembranous colitis in North American and European countries (4, 5). We found these strains, together with seven additional pulsed-field gel electrophoresis (PFGE) patterns, among 37 isolates recently recovered from patients with AAD in a Costa Rican hospital. Herein we present the macrorestriction patterns of the isolates as well as data regarding their toxin genotypes and susceptibility to selected antibiotics. The isolates were recovered by inoculating a loopful of diarrheic stool samples onto cefoxitin-cycloserine fructose agar plates (CCFA; Oxoid). They were identified with the rapid ID32A system (bioMerieux) and a PCR targeting the triose phosphate isomerase gene (9). We typed the isolates by PFGE (1) and amplified fragments of the tcdA, tcdB, tcdC, and cdtB genes by PCR with oligonucleotides and conditions reported elsewhere (3, 9, 10). These genes code for toxin A, toxin B, the negative regulator of the pathogenicity locus, and the binding domain of the binary toxin, respectively. MICs of clindamycin, metronidazole, vancomycin, moxifloxacin, ciprofloxacin, and amoxicillin-clavulanate were determined using Etest strips (AB bioMerieux). C. difficile ATCC 700057 and Bacteroides fragilis ATCC 25285 were used as reference strains. The breakpoints recommended by the CLSI (2) and Pelaez et al. (6) were used for antimicrobial susceptibility interpretation. Nine different PFGE types were identified in the collection (Fig. ​(Fig.1).1). All isolates were positive for both tcdA and tcdB and susceptible to vancomycin and metronidazole, which are the first antibiotics to be prescribed for this type of infection. They were also susceptible to amoxicillin-clavulanic acid. FIG. 1. Pulsed-field gel electrophoresis. SmaI dendrogram of 37 isolates of Clostridium difficile recovered from patients with antibiotic-associated diarrhea in a Costa Rican hospital. More than half the isolates exhibited the macrorestriction pattern of the NAP1 strain (n = 20; 54%). All these bacteria had the gene for the binding domain of the binary toxin and a deletion in tcdC (Table ​(Table1).1). Only one of the NAP1 strains was susceptible to clindamycin. In fact, 10 (50%) were categorized as intermediate and another 9 (45%) as resistant to this antibiotic. One clindamycin-resistant NAP1 strain had a MIC of ≥256 μg ml−1. In agreement with recent data (7), all NAP1 isolates were highly resistant to the two fluoroquinolones tested (Table ​(Table11). TABLE 1. SmaI macrorestriction patterns, toxin genotype, and MICs of clindamycin, metronidazole, vancomycin, moxifloxacin, ciprofloxacin, and amoxicillin-clavulanic acid of 37 isolates of C. difficile recovered from patients with antibiotic-associated diarrhea ... The 13 isolates with the SmaI patterns 447, 448, 449, and 452 clustered together (Fig. ​(Fig.1).1). These 13 isolates lacked the binary toxin and had a deletion in the tcdC gene (Table ​(Table1).1). In addition, they were without exception categorized as highly resistant to clindamycin, moxifloxacin, and ciprofloxacin (Table ​(Table1).1). One strain with the PFGE pattern 447 and the strains with the PFGE patterns 449 and 452 exhibited the highest MICs to vancomycin overall. The isolates with the PFGE patterns 100, 450, and 451 (NAP2) and 247 (NAP9) were negative for the binary toxin and did not have deletions in the tcdC gene (Table ​(Table1).1). The strains with the PFGE patterns 100, 450, and 247 (NAP9) were resistant to clindamycin, moxifloxacin, and ciprofloxacin (Table ​(Table1).1). In contrast, the isolate with the PFGE pattern 451 (NAP2) was moderately resistant to clindamycin (MIC = 16 μg ml−1) and highly resistant to ciprofloxacin (MIC > 32 μg ml−1) but susceptible to moxifloxacin (MIC = 2 μg ml−1). NAP9 strains have been recently isolated from retail meat (8). The finding of the NAP1 strain in Latin American countries is novel and deserves attention from infectious disease specialists and epidemiologists to prevent its dissemination.

Farming of seaweeds

Abstract: Seaweed farming at sea is becoming an increasingly competitive biomass production candidate for food and related uses. With exponential growth over recent decades, farmed seaweed output reached 24 million tons by 2012. However, just eight Asian nations produced 99% of that while most of the world’s 150 countries and territories with coasts were yet to begin seaweed farming. Using current technology, extensively available sea areas may be cultivated to produce crops that require no freshwater or fertilizers, while providing a variety of valuable ecosystem services. Following a deductive or principle-based approach that establishes primary production from seaweed biosynthesis as a basis of food production, this chapter describes the fundamentals of seaweed farming, harvest and postharvest techniques, ecological and economic considerations, and a perspective on opportunities and challenges. The objective is to provide both an overall account of the state of the art on seaweed farming as well as a contribution to the industry’s sustainable development.

Total and Added Sugar Intake: Assessment in Eight Latin American Countries

Abstract: Non-communicable diseases are growing at an alarming rate in Latin America. We assessed total and added sugar intake in Argentina, Brazil, Chile, Colombia, Costa Rica, Ecuador, Peru, and Venezuela, to verify the adequacy of the World Health Organization’s recommendations, considering gender, socioeconomic level (SEL) and age. A total of 9218 non-institutionalized individuals living in urban areas (age range 15–65 years) were assessed in the Latin American Study of Nutrition and Health (ELANS), a multicenter household population-based cross-sectional survey. Socio-demographic data were collected. Total and added sugar intakes were measured using two non-consecutive 24-h dietary recalls. The prevalence of excessive sugar intake was estimated. A large proportion of individuals showed high consumption of total and added sugar intake, which reflected in the high prevalence of excessive sugar intake. With minimal differences across countries, in general, women, individuals with high SEL, and younger people had higher percentages of total energy intake from total and added sugar intake, and of contribution of carbohydrates from total and added sugars. Thus, there is high consumption of total and added sugar intake in the Latin American countries with some peculiarities considering socio-demographic variables, which should be considered in each country’s health intervention proposals.

Neutralización del efecto hemorrágico inducido por veneno de Bothrops asper (Serpentes: Viperidae) por extractos de plantas tropicales

Abstract: Organic extracts representing 48 species included in 30 families of Costa Rican tropicalp1ants were evaluated fortheir abiJity to neutralize hemorrhagic acfivity lnduc.ed by the venom of the snake Bothrops aspero Abioassay in mice was used, based ortintraderrnal injection of either venom orvenom-extract mixtiin:l.s followed py the rneasurement ofhemorrhagic areas: Total inhibition of henmrrhage wasobsetved with the ethanolic;ethyl and .aqueous extractsiofBur;w{i simaruba, Clusia torresii, C. pafmana, Croton·4ram, Persea qmericanq, Phoebebrenesu,Pimentadioica, . . Sapindussaponariai Smifax cuculmeco and Virola koschnyi; C:hemicalanalysis of these extracts identified catequines,flavones, anthocyanines